Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,827,053	G→A	C221Y (TGT→TAT)	srlE →	sorbitol‑specific PTS enzyme IIBC1 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,827,053	0	G	A	100.0%	245.9 / NA	69	C221Y (TGT→TAT)	srlE	sorbitol‑specific PTS enzyme IIBC1 component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/29);  total (40/29)

ACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCC  >  NC_000913/2826917‑2827183                                                                                                                                         |                                                                                                                                   aCCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTAttc                                                                                                                                 >  1:918793/1‑140 (MQ=255) aCCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTAttc                                                                                                                                 >  2:311805/1‑140 (MQ=255) aCCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTAttc                                                                                                                                 >  2:834740/1‑140 (MQ=255)   cATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTTCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTAttcct                                                                                                                               >  1:399698/1‑140 (MQ=255)   cATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTAttcct                                                                                                                               >  1:596950/1‑140 (MQ=255)   cATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTAttcct                                                                                                                               >  1:519935/1‑140 (MQ=255)   cATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTAttcct                                                                                                                               >  2:348604/1‑140 (MQ=255)   cATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTAttcct                                                                                                                               >  1:63426/1‑140 (MQ=255)   cATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTAttcct                                                                                                                               >  1:1026729/1‑140 (MQ=255)   cATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTAttcct                                                                                                                               <  2:355374/140‑1 (MQ=255)   cATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGCCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTAttcct                                                                                                                               >  1:839712/1‑140 (MQ=255)     ttCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTAttccttc                                                                                                                             >  2:951041/1‑140 (MQ=255)        tGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCa                                                                                                                          <  1:951041/140‑1 (MQ=255)                    cATTCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCt                                                                                                              <  1:318944/140‑1 (MQ=255)                    cATTCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCt                                                                                                              <  1:598110/140‑1 (MQ=255)                       tCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTc                                                                                                           >  1:13058/1‑140 (MQ=255)                       tCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTc                                                                                                           >  2:454787/1‑140 (MQ=255)                         gTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCt                                                                                                         <  2:447079/140‑1 (MQ=255)                              ggCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGcc                                                                                                    >  2:438276/1‑140 (MQ=255)                              ggCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGcc                                                                                                    <  2:629025/140‑1 (MQ=255)                              ggCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGcc                                                                                                    <  2:519935/140‑1 (MQ=255)                              ggCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGcc                                                                                                    <  1:915732/140‑1 (MQ=255)                              ggCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGcc                                                                                                    >  1:39140/1‑140 (MQ=255)                              ggCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTCCCTCGGcc                                                                                                    <  1:390229/140‑1 (MQ=255)                                  cTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAgg                                                                                                <  1:438276/140‑1 (MQ=255)                                          catcatTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTa                                                                                        >  1:904404/1‑140 (MQ=255)                                             catTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCg                                                                                     >  1:526754/1‑140 (MQ=255)                                             catTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCg                                                                                     <  1:454787/140‑1 (MQ=255)                                              atTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGc                                                                                    >  2:519013/1‑140 (MQ=255)                                               ttATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGcc                                                                                                    >  1:891778/1‑123 (MQ=255)                                               ttATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGcc                                                                                                    <  2:891778/123‑1 (MQ=255)                                               ttATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGca                                                                                   <  2:63426/140‑1 (MQ=255)                                               ttATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGca                                                                                   <  2:399698/140‑1 (MQ=255)                                               ttATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGca                                                                                   >  2:1039818/1‑140 (MQ=255)                                               ttATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGca                                                                                   <  2:39140/140‑1 (MQ=255)                                               ttATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGca                                                                                   >  1:478884/1‑140 (MQ=255)                                                 aTGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcaca                                                                                 >  2:149737/1‑140 (MQ=255)                                                      ttCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCTCCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTa                                                                            <  2:791529/140‑1 (MQ=255)                                                       tCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTAt                                                                           >  1:765769/1‑140 (MQ=255)                                                       tCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTAt                                                                           <  1:1039818/140‑1 (MQ=255)                                                        cTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATc                                                                          >  1:614586/1‑140 (MQ=255)                                                         tGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCg                                                                         <  2:614586/140‑1 (MQ=255)                                                         tGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCg                                                                         >  2:128949/1‑140 (MQ=255)                                                         tGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCg                                                                         <  1:149737/140‑1 (MQ=255)                                                           gCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGc                                                                       <  2:1026729/140‑1 (MQ=255)                                                                     cTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgattg                                                             <  1:519013/140‑1 (MQ=255)                                                                     cTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgattg                                                             <  1:348604/140‑1 (MQ=255)                                                                         aTTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGt                                                         <  1:834740/140‑1 (MQ=255)                                                                              ccACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGa                                                    <  2:979072/140‑1 (MQ=255)                                                                                       tGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCg                                           >  1:439150/1‑140 (MQ=255)                                                                                              cTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAatat                                    >  2:386458/1‑140 (MQ=255)                                                                                              cTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAatat                                    >  2:884466/1‑140 (MQ=255)                                                                                              cTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAatat                                    >  1:741899/1‑140 (MQ=255)                                                                                                  cGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccg                                >  2:165885/1‑140 (MQ=255)                                                                                                   gAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccgc                               <  2:439150/140‑1 (MQ=255)                                                                                                   gAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccgc                               <  2:13058/140‑1 (MQ=255)                                                                                                                ggTCTGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGA‑TGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTa                 >  1:301206/1‑140 (MQ=255)                                                                                                                ggTCTGGTCATGCCGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCttt                  >  1:430807/1‑140 (MQ=255)                                                                                                                    tGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCg              >  2:891802/1‑140 (MQ=255)                                                                                                                    tGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCg              >  1:734826/1‑140 (MQ=255)                                                                                                                    tGGTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGA‑TGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCgg             <  2:301206/140‑1 (MQ=255)                                                                                                                     ggTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCgg             <  2:749310/140‑1 (MQ=255)                                                                                                                     ggTCATGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCgg             <  1:165885/140‑1 (MQ=255)                                                                                                                          tGCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTg        >  1:897601/1‑140 (MQ=255)                                                                                                                           gCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCAc          <  2:300151/137‑1 (MQ=255)                                                                                                                           gCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCAc          >  1:300151/1‑137 (MQ=255)                                                                                                                           gCTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGt       >  2:429954/1‑140 (MQ=255)                                                                                                                            cTGGCGCTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGtt      >  2:657428/1‑140 (MQ=255)                                                                                                                                cgcTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGcc  >  1:67977/1‑140 (MQ=255)                                                                                                                                cgcTCATCTATTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGcc  >  1:919329/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                   ACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGCATCATTATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGGCACTGTTTGCC  >  NC_000913/2826917‑2827183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑