Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,836,487	T→C	E313E (GAA→GAG)	hypF ←	carbamoyltransferase HypF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,836,487	0	T	C	100.0%	172.1 / NA	48	E313E (GAA→GAG)	hypF	carbamoyltransferase HypF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/27);  total (21/27)

CCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTATTTTTTATCCACCAGCACAATCGGCGCGGCGGGCG  >  NC_000913/2836352‑2836617                                                                                                                                        |                                                                                                                                   ccGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCt                                                                                                                                >  1:627996/1‑140 (MQ=255)       caggATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCACTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGATTACTGCTCAGGTTGCCTGAGGGCGTCACCAGCGGGCATTGCAGCTCCTGTAACa                                                                                                                          <  2:950557/137‑1 (MQ=255)       cACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACa                                                                                                                          <  1:661562/140‑1 (MQ=255)       cACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACa                                                                                                                          >  2:291151/1‑140 (MQ=255)         cGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGc                                                                                                                        <  2:954670/140‑1 (MQ=255)               cgcgGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCCGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGc                                                                                                                  >  1:688348/1‑140 (MQ=255)                 cgGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCTCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTg                                                                                                                >  2:907143/1‑140 (MQ=255)                      aTGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCg                                                                                                           >  1:348979/1‑140 (MQ=255)                      aTGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCg                                                                                                           >  1:290052/1‑140 (MQ=255)                       tGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCgg                                                                                                          >  2:30628/1‑140 (MQ=255)                           tCAAGAAACCGTCGGCAAAGCCCCGCCAATCCGCCAGCGCCTGTTCGTTGCTGATAGCCGGGGGTTTTCCCCGCCAGTTGCCGGAGGGCATCACCAACGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGGGCGGGGTc                                                                                                      >  2:1015235/1‑140 (MQ=255)                              aGAATCCGTCGGCAATGCCCTGCAAATCCTCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCa                                                                                                   <  1:907143/140‑1 (MQ=255)                              aGAATCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCa                                                                                                   <  2:434036/140‑1 (MQ=255)                                aaTCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAgg                                                                                                 <  1:30628/140‑1 (MQ=255)                                aaTCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAgg                                                                                                 <  1:816836/140‑1 (MQ=255)                                  tCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCCGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCTGGCa                                                                                               >  1:956454/1‑140 (MQ=255)                                   ccGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCaa                                                                                              >  1:221957/1‑140 (MQ=255)                                      tCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACAt                                                                                           >  2:325661/1‑140 (MQ=255)                                               cccTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGAc                                                                                  <  1:103614/140‑1 (MQ=255)                                                ccTGCAAATCCGCCAGCGCCTGTTCGTTGCCGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACt                                                                                 <  2:688348/140‑1 (MQ=255)                                                    cAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGa                                                                                   >  1:900199/1‑134 (MQ=255)                                                    cAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGa                                                                                   <  2:900199/134‑1 (MQ=255)                                                    cAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGt                                                                             <  2:290052/140‑1 (MQ=255)                                                     aaaTCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGtt                                                                            <  2:221957/140‑1 (MQ=255)                                                     aaaTCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGtt                                                                            <  2:348979/140‑1 (MQ=255)                                                      aaTCCGCCAGAGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCAGGAGGTCATCCCCAGCGGGCATTGCAGCTCCTGTCACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTa                                                                           <  2:693867/140‑1 (MQ=255)                                                          cGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGt                                                                       >  1:1017056/1‑140 (MQ=255)                                                          cGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGt                                                                       <  2:627996/140‑1 (MQ=255)                                                            ccAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTTCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGtt                                                                            >  1:344078/1‑133 (MQ=255)                                                            ccAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTTCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGtt                                                                            <  2:344078/133‑1 (MQ=255)                                                            ccAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCa                                                                     <  2:949186/140‑1 (MQ=255)                                                                cgcCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAgggg                                                                 >  2:674845/1‑140 (MQ=255)                                                                 gcCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGc                                                                <  1:318808/140‑1 (MQ=255)                                                                         gTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGTGatgatat                                                        <  1:358010/140‑6 (MQ=255)                                                                             ccgATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATcaca                                                    <  2:956454/138‑1 (MQ=255)                                                                                  aGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTTCCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCt                                               <  2:420487/140‑1 (MQ=255)                                                                                  aGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCt                                               >  1:334418/1‑140 (MQ=255)                                                                                  aGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCt                                               >  1:309397/1‑140 (MQ=255)                                                                                       gtggtTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGa                                          >  2:504509/1‑140 (MQ=255)                                                                                              aCCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACTAAGCTCAGGAACGTAtt                                   <  2:431426/140‑1 (MQ=255)                                                                                                  cTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTAtttttt                               >  2:834015/1‑140 (MQ=255)                                                                                                   tCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTATTTTTTa                              <  2:334418/140‑1 (MQ=255)                                                                                                    cAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTATTTTTTAt                             >  1:567812/1‑140 (MQ=255)                                                                                                                       aCCAGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTATTTTTTATCCACCAGCACAATcggcgc          <  1:242135/140‑1 (MQ=255)                                                                                                                          aGCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTATTTTTTATCCACCAGCACAATcggcgcggc       <  1:325661/140‑1 (MQ=255)                                                                                                                           gCGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTATTTTTTATCCACCAGCACAATcggcgcggcg      >  1:341486/1‑140 (MQ=255)                                                                                                                            cGGGCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTATTTTTTATCCACCAGCACAATCGGCGcggcgg     >  2:576455/1‑140 (MQ=255)                                                                                                                               gCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCTGGAACGTATTTTTTATCCACCAGCACAATCGGCGCggcgggcg  <  2:1011730/140‑1 (MQ=255)                                                                                                                               gCATTGCAGCTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTATTTTTTATCCACCAGCACAATCGGCGCggcgggcg  <  1:674845/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   CCGCTGCACGATGTCGCGGTTATGTATCAAGAATCCGTCGGCAATGCCCTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTATTTTTTATCCACCAGCACAATCGGCGCGGCGGGCG  >  NC_000913/2836352‑2836617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑