Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	385,891	C→T	V220V (GTC→GTT)	tauA →	taurine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	385,891	0	C	T	100.0%	96.4 / NA	28	V220V (GTC→GTT)	tauA	taurine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/11);  total (17/11)

TGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGTGTGGCTGAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAAGGTGACGTTCCGGGG  >  NC_000913/385757‑386026                                                                                                                                       |                                                                                                                                        tGGGCACCGGCGGTTTACCCCCCCGAAAAAAACGGCAAGGGGTTGACCGATTCTTAACAGGTCGGGCAGGGGGGCGCGCCAACGCCGGGCGTCTGGGGGGGGCGCAAAGAATTTGCCGAGAAACATCCTTAGGGTGTGaa                                                                                                                                    >  2:998611/1‑140 (MQ=255)   ggCACCGGCGGTTAACGCCCTGGGAAAAAACGGCAAGGGGTTGGCCGATTCTGAAAAGGGCGGGGGGTGGGGGGCGCCCACGCTGGACGGCTGGGTGGTGCGCAAAGATTTTTCCGGGAAAAATCCTGAGGGTGTGAAAg                                                                                                                                  >  2:819877/1‑140 (MQ=255)                aaCGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAg                                                                                                                     <  1:228592/140‑1 (MQ=255)                        ggAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAgcgc                                                                                                                  >  2:728657/1‑135 (MQ=255)                        ggAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAgcgc                                                                                                                  <  1:728657/135‑1 (MQ=255)                               gACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCa                                                                                                      >  2:437824/1‑140 (MQ=255)                                              aCCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGc                                                                                       <  1:120464/140‑1 (MQ=255)                                                  aTTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTa                                                                                             >  2:737116/1‑130 (MQ=255)                                                  aTTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTa                                                                                             <  1:737116/130‑1 (MQ=255)                                                        aaCAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGAc                                                                             >  2:413086/1‑140 (MQ=255)                                                             gTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGtgtg                                                                        >  2:83320/1‑140 (MQ=255)                                                             gTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGtgtg                                                                        >  2:501446/1‑140 (MQ=255)                                                             gTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGtgtg                                                                        >  2:143563/1‑140 (MQ=255)                                                             gTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGtgtg                                                                        >  2:880438/1‑140 (MQ=255)                                                             gTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGtgtg                                                                        >  1:749455/1‑140 (MQ=255)                                                                       ggggCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGTGTGGCTGAAACAg                                                              >  2:1006935/1‑140 (MQ=255)                                                                            gcgcCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGTGTGGCTGAAACAGCCGGa                                                         >  1:168516/1‑140 (MQ=255)                                                                             cgcCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGTGTGGCTGAAACAGCCGGaa                                                        <  1:143563/140‑1 (MQ=255)                                                                                cAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGTGTGGCTGAAACAGCCGGAAAAc                                                     >  1:439398/1‑140 (MQ=255)                                                                                             cTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGTGTGGCTGAAACAGCCGGAAAACATCAGCAAACTgg                                        <  1:793822/140‑1 (MQ=255)                                                                                              tGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGTGTGGCTGAAACAGCCGGAAAACATCAGCAAACTGgc                                       >  1:764891/1‑140 (MQ=255)                                                                                                       cgcAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGTGTGGCTGAAACAGCCGGAAAACATCAGCAAACTGgcg                                      <  2:636790/132‑1 (MQ=255)                                                                                                       cgcAAAGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGTGTGGCTGAAACAGCCGGAAAACATCAGCAAACTGgcg                                      >  1:636790/1‑132 (MQ=255)                                                                                                          aaaGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGTGTGGCTGAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAgcgg                           >  2:331768/1‑140 (MQ=255)                                                                                                           aaGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGTGTGGCTGAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAgcggc                          <  1:1006935/140‑1 (MQ=255)                                                                                                            aGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGTGTGGCTGAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAgcggc                          >  2:851651/1‑139 (MQ=255)                                                                                                            aGATTTTGCCGAGAAACATCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGTGTGGCTGAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAgcggc                          <  1:851651/139‑1 (MQ=255)                                                                                                                              tCCTGAGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGTGTGGCTGAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAAGGTGACGTTc       <  2:168516/140‑1 (MQ=255)                                                                                                                                   aGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGTGTGGCTGAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAAGGTGACGTTCCgggg  >  2:445209/1‑140 (MQ=255)                                                                                                                                   aGGTTGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGTGTGGCTGAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAAGGTGACGTTCCgggg  <  1:501446/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTCGTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGACGTGTGGCTGAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAAGGTGACGTTCCGGGG  >  NC_000913/385757‑386026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑