Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,971,378	C→T	T36T (ACC→ACT)	ygdR →	DUF903 domain‑containing lipoprotein YgdR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,971,378	0	C	T	100.0%	333.1 / NA	92	T36T (ACC→ACT)	ygdR	DUF903 domain‑containing lipoprotein YgdR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/49);  total (43/49)

TCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACCGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAATTATTGAACGTTAACAAATAAGGTCAGCATCCGGC  >  NC_000913/2971242‑2971510                                                                                                                                         |                                                                                                                                     cctatgataggCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGAt                                                                                                                                   >  1:873981/8‑140 (MQ=255)    taggtaggCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGTAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGa                                                                                                                                >  2:913603/1‑140 (MQ=255)    taggtaggCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGTAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGa                                                                                                                                <  1:913603/140‑1 (MQ=255)    taggtaggCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGa                                                                                                                                <  1:887562/140‑1 (MQ=255)    taggtaggCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGa                                                                                                                                >  1:366159/1‑140 (MQ=255)      ggtaggCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGaaa                                                                                                                              >  2:1002342/1‑140 (MQ=255)        taggCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAAc                                                                                                                            >  1:404413/1‑140 (MQ=255)        taggCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAAc                                                                                                                            >  2:683055/1‑140 (MQ=255)        taggCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAAc                                                                                                                            >  2:619262/1‑140 (MQ=255)          ggCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCt                                                                                                                          >  1:326723/1‑140 (MQ=255)           gCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCt                                                                                                                          <  1:273488/139‑1 (MQ=255)           gCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCt                                                                                                                          >  2:273488/1‑139 (MQ=255)           gCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTg                                                                                                                         >  1:476278/1‑140 (MQ=255)           gCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTg                                                                                                                         >  2:473858/1‑140 (MQ=255)           gCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTg                                                                                                                         >  2:299011/1‑140 (MQ=255)           gCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTg                                                                                                                         >  2:241016/1‑140 (MQ=255)           gCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTg                                                                                                                         >  2:15761/1‑140 (MQ=255)           gCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTg                                                                                                                         >  1:919008/1‑140 (MQ=255)           gCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTg                                                                                                                         >  1:766508/1‑140 (MQ=255)           gCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTg                                                                                                                         >  2:914169/1‑140 (MQ=255)           gCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTg                                                                                                                         >  2:817615/1‑140 (MQ=255)           gCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTg                                                                                                                         >  2:658073/1‑140 (MQ=255)           gCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTg                                                                                                                         >  2:671313/1‑140 (MQ=255)           gCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTg                                                                                                                         >  2:368153/1‑140 (MQ=255)           gCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTg                                                                                                                         <  1:184756/140‑1 (MQ=255)              aaaTGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGaaa                                                                                                                      <  1:619262/140‑1 (MQ=255)                 tGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATtg                                                                                                                   <  1:368153/140‑1 (MQ=255)                  gAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATtga                                                                                                                  <  2:366159/140‑1 (MQ=255)                  gAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATtga                                                                                                                  <  1:208718/140‑1 (MQ=255)                     gATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATtgatga                                                                                                               >  1:54942/1‑140 (MQ=255)                     gATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATtgatga                                                                                                               <  1:817615/140‑1 (MQ=255)                     gATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATtgatga                                                                                                               >  2:545176/1‑140 (MQ=255)                          ccGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATa                                                                                                          <  1:932712/140‑1 (MQ=255)                          ccGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATa                                                                                                          <  2:766508/140‑1 (MQ=255)                          ccGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATa                                                                                                          >  2:630782/1‑140 (MQ=255)                            ggATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATAcc                                                                                                        <  2:983673/140‑1 (MQ=255)                            ggATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATAcc                                                                                                        <  1:15761/140‑1 (MQ=255)                            ggATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATAcc                                                                                                        <  1:519940/140‑1 (MQ=255)                             gATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATtgatga                                                                                                               <  2:209644/132‑1 (MQ=255)                             gATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATtgatga                                                                                                               >  1:209644/1‑132 (MQ=255)                              aTGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCgg                                                                                                      <  1:482210/140‑1 (MQ=255)                               tGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCggg                                                                                                     >  2:278922/1‑140 (MQ=255)                                  aaaaaTGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTg                                                                                                  <  2:873981/140‑1 (MQ=255)                                   aaaaTGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTgg                                                                                                 <  1:724223/140‑1 (MQ=255)                                    aaaTGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCACTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGt                                                                                                <  2:919008/140‑1 (MQ=255)                                    aaaTGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTT‑CGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTg                                                                                               <  2:100442/140‑1 (MQ=255)                                       tGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAg                                                                                             <  2:332373/140‑1 (MQ=255)                                          gCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTa                                                                                          <  1:671313/140‑1 (MQ=255)                                             gTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCa                                                                                       >  1:55041/1‑140 (MQ=255)                                              taataaTTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCAc                                                                                      >  2:693729/1‑140 (MQ=255)                                               aataatTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACg                                                                                     <  1:658073/140‑1 (MQ=255)                                               aataatTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACg                                                                                     <  1:392087/140‑1 (MQ=255)                                                 taatTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGAt                                                                                   <  1:683055/140‑1 (MQ=255)                                                    tttCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAg                                                                                <  1:278922/140‑1 (MQ=255)                                                    tttCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAg                                                                                <  1:693729/140‑1 (MQ=255)                                                        tGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCaa                                                                                >  1:280027/1‑135 (MQ=255)                                                        tGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCaa                                                                                <  2:280027/136‑2 (MQ=255)                                                        tGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAg                                                                            >  2:484004/1‑140 (MQ=255)                                                        tGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAg                                                                            >  1:656987/1‑140 (MQ=255)                                                         gCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAgg                                                                           <  2:404413/140‑1 (MQ=255)                                                             tCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCa                                                                                 >  2:910005/1‑130 (MQ=255)                                                             tCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCa                                                                                 <  1:910005/130‑1 (MQ=255)                                                             tCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAAc                                                                       >  2:941758/1‑140 (MQ=255)                                                               ggACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAAcgc                                                                     <  1:545176/140‑1 (MQ=255)                                                               ggACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAAcgc                                                                     <  2:476278/140‑1 (MQ=255)                                                                         ttGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAAtt                                                           <  1:941758/140‑1 (MQ=255)                                                                            cTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAAc                                                        <  2:54942/140‑1 (MQ=255)                                                                                  ccGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGtgat                                                  >  2:658879/1‑140 (MQ=255)                                                                                              gTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCaa                                      <  1:658879/140‑1 (MQ=255)                                                                                                 aTTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAatt                                   <  2:249493/140‑1 (MQ=255)                                                                                                 aTTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAatt                                   >  1:131157/1‑140 (MQ=255)                                                                                                   tACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAattat                                 <  1:1033396/140‑1 (MQ=255)                                                                                                     cGTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAATTATTg                               >  1:628942/1‑140 (MQ=255)                                                                                                      gTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCa                                       <  2:841958/131‑1 (MQ=255)                                                                                                      gTCATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCa                                       >  1:841958/1‑131 (MQ=255)                                                                                                        cATGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAATTATTGAAc                            <  1:473858/140‑1 (MQ=255)                                                                                                         aTGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAattatt                                <  2:330069/135‑1 (MQ=255)                                                                                                         aTGGCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAattatt                                >  1:330069/1‑135 (MQ=255)                                                                                                         aTGACGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAATTATTGAACg                           <  2:771753/140‑1 (MQ=255)                                                                                                           ggCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAattatt                                <  1:442770/133‑1 (MQ=255)                                                                                                           ggCGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAattatt                                >  2:442770/1‑133 (MQ=255)                                                                                                             cGACCAAAGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAATTATTGAACGTTaa                       <  2:878020/140‑1 (MQ=255)                                                                                                                  aaaGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAATTATTGAACGTTAACAAAt                  <  1:514470/140‑1 (MQ=255)                                                                                                                   aaGATGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAATTATTGAACGTTAACAAATa                 <  1:484004/140‑1 (MQ=255)                                                                                                                       tGGCCGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAATTATTGAACGTTAACAAATAAGGt             <  2:628942/140‑1 (MQ=255)                                                                                                                          ccGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAATTATTGAACGTTAACAAATAAGGTCAg          >  1:1017871/1‑140 (MQ=255)                                                                                                                          ccGTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAATTATTGAACGTTAACAAATAAGGGCAg          >  1:1004181/1‑140 (MQ=255)                                                                                                                            gTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAATTATTGAACGTTAACAAATAAGGTCAGCa        <  1:299011/140‑1 (MQ=255)                                                                                                                            gTATGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAATTATTGAACGTTAACAAATAAGGTCAGCa        <  2:335413/140‑1 (MQ=255)                                                                                                                              aTGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAATTATTGAACGTTAACAAATAAGGTCAGCATc      >  1:506436/1‑140 (MQ=255)                                                                                                                               tGATTTTGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAATTATTGAACGTTAACAAATAAGGTCAGCATcc     >  2:60840/1‑140 (MQ=255)                                                                                                                               tGATTTTGACTGATGGAAAACCCGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAATTATTGAACGTTAACAAATAAGGTCAGCATcc     >  2:893517/1‑140 (MQ=255)                                                                                                                                  ttttGACTGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAATTATTGAACGTTAACAAATAAGGTCAGCATCCGGc  <  1:60840/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCTGCAGTCGGACTGGCGTTTGCTGTTTCCGGGTGTTCCAGTGATTACGTCATGGCGACCAAAGATGGCCGTATGATTTTGACCGATGGAAAACCTGAAATTGATGATGATACCGGGCTGGTGAGTTATCACGATCAGCAAGGTAACGCGATGCAAATTAACCGTGATGATGTTTCGCAAATTATTGAACGTTAACAAATAAGGTCAGCATCCGGC  >  NC_000913/2971242‑2971510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑