Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,216,837	G→A	C20Y (TGC→TAC)	nfeR →	DNA‑binding transcriptional repressor NfeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,216,837	0	G	A	100.0%	177.7 / NA	50	C20Y (TGC→TAC)	nfeR	DNA‑binding transcriptional repressor NfeR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/23);  total (27/23)

AAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAACGTTTCTTTGGTCACGGTGAATTACGT  >  NC_000913/3216701‑3216970                                                                                                                                         |                                                                                                                                      aaaaTCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAGTTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCCCGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGctactg                                                                                                                                    <  2:675346/140‑1 (MQ=255)     tCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCaaa                                                                                                                                <  1:987024/140‑1 (MQ=255)                 ttGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCa                                                                                                                    <  1:447799/140‑1 (MQ=255)                  tGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAg                                                                                                                   <  2:379795/140‑1 (MQ=255)                  tGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAg                                                                                                                   <  2:786956/140‑1 (MQ=255)                         aCGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGa                                                                                                            >  2:657126/1‑140 (MQ=255)                             tCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAg                                                                                                                   <  2:293462/129‑1 (MQ=255)                             tCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAg                                                                                                                   >  1:293462/1‑129 (MQ=255)                                  tCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCgg                                                                                                   <  1:657126/140‑1 (MQ=255)                                    aGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGac                                                                                                 <  2:547362/140‑1 (MQ=255)                                     gATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAAGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGaca                                                                                                >  2:1025834/1‑140 (MQ=255)                                      atatAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAAGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGacac                                                                                               <  1:1025834/140‑1 (MQ=255)                                       tataAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACg                                                                                              <  1:638160/140‑1 (MQ=255)                                         taAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGt                                                                                            >  1:421852/1‑140 (MQ=255)                                            aTTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCAc                                                                                         <  1:390585/140‑1 (MQ=255)                                                gATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGc                                                                                     >  1:939588/1‑140 (MQ=255)                                                gATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGc                                                                                     >  1:444978/1‑140 (MQ=255)                                                     atCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGaa                                                                                >  1:611601/1‑140 (MQ=255)                                                       cTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAaca                                                                              >  1:996713/1‑140 (MQ=255)                                                          aTTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACgg                                                                           >  2:49093/1‑140 (MQ=255)                                                          aTTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACgg                                                                           >  1:506806/1‑140 (MQ=255)                                                          aTTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACgg                                                                           >  2:831742/1‑140 (MQ=255)                                                           ttAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGt                                                                          <  1:521967/140‑1 (MQ=255)                                                           ttAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGt                                                                          <  2:634419/140‑1 (MQ=255)                                                           ttAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGt                                                                          <  1:831742/140‑1 (MQ=255)                                                           ttAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGt                                                                          <  1:49093/140‑1 (MQ=255)                                                           ttAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGt                                                                          <  1:154586/140‑1 (MQ=255)                                                             aaGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGt                                                                          <  2:902048/138‑1 (MQ=255)                                                             aaGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGt                                                                          >  1:968237/1‑138 (MQ=255)                                                             aaGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGt                                                                          >  1:902048/1‑138 (MQ=255)                                                             aaGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGt                                                                          <  2:968237/138‑1 (MQ=255)                                                                    aggaggCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAAtgctgc                                                                 >  1:134726/1‑140 (MQ=255)                                                                         gCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCggtgg                                                            >  1:299860/1‑140 (MQ=255)                                                                         gCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCggtgg                                                            >  2:321302/1‑140 (MQ=255)                                                                              tatGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGcc                                                       >  1:5767/1‑140 (MQ=255)                                                                                tGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCAc                                                     <  2:996713/140‑1 (MQ=255)                                                                                       tcatcaCGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTcgcg                                              >  1:170768/1‑140 (MQ=255)                                                                                       tcatcaCGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTcgcg                                              >  1:166119/1‑140 (MQ=255)                                                                                                 gggTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGgtcg                                    >  1:891767/1‑140 (MQ=255)                                                                                                  ggTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGgtcgt                                   <  2:506806/140‑1 (MQ=255)                                                                                                   gtgtTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGgtcgtc                                  >  2:108472/1‑140 (MQ=255)                                                                                                   gtgtTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGgtcgtc                                  >  1:640367/1‑140 (MQ=255)                                                                                                               tGAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAACGTTTCt                      <  2:134726/140‑1 (MQ=255)                                                                                                                gAAGGCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGGCGTCGGCAACGTTTCtt                     >  1:577482/1‑140 (MQ=255)                                                                                                                   ggCCAGCCACGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAACGTTTCTTTgg                  >  1:775042/1‑140 (MQ=255)                                                                                                                    gccagccaCGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAACGTTTCTTTGGt                 <  2:577482/140‑1 (MQ=255)                                                                                                                     ccagccaCGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAACGTTTCTTTGGTc                <  2:421852/140‑1 (MQ=255)                                                                                                                     ccagccaCGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAACGTTTCTTTGGTc                <  2:939588/140‑1 (MQ=255)                                                                                                                        gccaCGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAACGTTTCTTTGGTCACg             >  1:316400/1‑140 (MQ=255)                                                                                                                         ccaCGCCATGAGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAACGTTTCTTTGGTCACgg            >  1:495951/1‑140 (MQ=255)                                                                                                                                   aGGGCTACTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAACGTTTCTTTGGTCACGGTGAATTACGt  >  1:433008/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      AAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAACATGAAGGCCAGCCACGCCATGAGGGCTGCTGCAAAGGTGAGAAGTCAGAACACGAGCACTGCGGACACGGTCACCAGCATGAACACGGTCAATGCTGCGGTGGTCGCCACGGTCGCGGCGGCGGTCGTCGGCAACGTTTCTTTGGTCACGGTGAATTACGT  >  NC_000913/3216701‑3216970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑