Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,296,870	T→C	G154G (GGT→GGC)	yraP →	divisome‑associated lipoprotein YraP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,296,870	0	T	C	100.0%	281.7 / NA	76	G154G (GGT→GGC)	yraP	divisome‑associated lipoprotein YraP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/40);  total (36/40)

ACGAGATTCGTCAGGGCCAGCCGATTGGTCTGGGCGAAGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCT  >  NC_000913/3296734‑3297002                                                                                                                                         |                                                                                                                                     aCGAGATTCGTCAGGGCCAGCCGATTGGTCTGGGCGAAGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGaa                                                                                                                                   <  1:346618/140‑1 (MQ=255)   gagaTTCGTCAGGGCCAGCCGATTGGTCTGGGCGAAGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAgt                                                                                                                                 >  1:415500/1‑140 (MQ=255)    agaTTCGTCAGGGCCAGCCGATTGGTCTGGGCGAAGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAgtg                                                                                                                                <  1:743399/140‑1 (MQ=255)     gaTTCGTCAGGGCCAGCCGATTGGTCTGGGCGAAGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGaa                                                                                                                                   <  2:314416/136‑1 (MQ=255)     gaTTCGTCAGGGCCAGCCGATTGGTCTGGGCGAAGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGaa                                                                                                                                   >  1:314416/1‑136 (MQ=255)     gaTTCGTCAGGGCCAGCCGATTGGTCTGGGCGAAGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAgtgt                                                                                                                               >  2:661133/1‑140 (MQ=255)       ttCGTCAGGGCCAGCCGATTGGTCTGGGCGAAGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTc                                                                                                                             >  1:304114/1‑140 (MQ=255)             aGGGCCAGCCGATTGGTCTGGGCGAAGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATg                                                                                                                       <  1:697720/140‑1 (MQ=255)             aGGGCCAGCCGATTGGTCTGGGCGAAGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATg                                                                                                                       <  1:949883/140‑1 (MQ=255)             aGGGCCAGCCGATTGGTCTGGGCGAAGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATg                                                                                                                       <  1:404496/140‑1 (MQ=255)                    gCCGATTGGTCTGGGCGAAGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTgg                                                                                                                >  1:65930/1‑140 (MQ=255)                        aTTGGTCTGGGCGAAGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGAc                                                                                                            <  2:889708/140‑1 (MQ=255)                               tGGGCGAAGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGt                                                                                                     <  1:126746/140‑1 (MQ=255)                                 ggCGAAGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTg                                                                                                                 >  1:374142/1‑126 (MQ=255)                                 ggCGAAGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTg                                                                                                                 <  2:374142/126‑1 (MQ=255)                                      aGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGa                                                                                              >  1:52224/1‑140 (MQ=255)                                         aTCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAg                                                                                           <  2:304114/140‑1 (MQ=255)                                               cGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGcggcgg                                                                                     <  2:128799/140‑1 (MQ=255)                                                  tACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAg                                                                                  >  1:757812/1‑140 (MQ=255)                                                  tACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAg                                                                                  >  2:954583/1‑140 (MQ=255)                                                    cGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGat                                                                                >  2:192304/1‑140 (MQ=255)                                                    cGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGat                                                                                >  2:796739/1‑140 (MQ=255)                                                     gTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAgcggc                                                                                       >  1:518688/1‑132 (MQ=255)                                                     gTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAgcggc                                                                                       <  2:518688/132‑1 (MQ=255)                                                        gATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAg                                                                                  <  1:1030038/134‑1 (MQ=255)                                                        gATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAg                                                                                  >  2:1030038/1‑134 (MQ=255)                                                             ccaccaAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGc                                                                       <  1:718662/140‑1 (MQ=255)                                                               accaAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCg                                                                     >  2:371813/1‑140 (MQ=255)                                                                ccaAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCgg                                                                    <  2:1033933/140‑1 (MQ=255)                                                                 caAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGCTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCggg                                                                   <  1:661133/140‑1 (MQ=255)                                                                   aaGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGACATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTg                                                                 >  2:177980/1‑140 (MQ=255)                                                                   aaGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTg                                                                 >  1:409273/1‑140 (MQ=255)                                                                    aGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGa                                                                >  1:602404/1‑140 (MQ=255)                                                                       gCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTg                                                                            <  2:293117/125‑1 (MQ=255)                                                                       gCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTg                                                                            >  1:293117/1‑125 (MQ=255)                                                                       gCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAgcg                                                             >  2:659348/1‑140 (MQ=255)                                                                        cGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAgcgg                                                            >  1:577605/1‑140 (MQ=255)                                                                        cGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAgcgg                                                            >  1:342214/1‑140 (MQ=255)                                                                        cGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAgcgg                                                            >  1:1025187/1‑140 (MQ=255)                                                                          ttCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAgcggcg                                                          <  2:415500/140‑1 (MQ=255)                                                                            cGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTg                                                        >  2:590067/1‑140 (MQ=255)                                                                             gCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGa                                                       <  1:875959/140‑1 (MQ=255)                                                                              cAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGaa                                                      <  2:342214/140‑1 (MQ=255)                                                                              cAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGaa                                                      <  1:796739/140‑1 (MQ=255)                                                                                gCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGc                                                    >  2:128153/1‑140 (MQ=255)                                                                                         cAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTa                                           <  1:192304/140‑1 (MQ=255)                                                                                           gCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACg                                         >  1:293397/1‑140 (MQ=255)                                                                                           gCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACg                                         >  2:924727/1‑140 (MQ=255)                                                                                            cGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAg                                                     >  2:835183/1‑127 (MQ=255)                                                                                            cGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAg                                                     <  1:835183/127‑1 (MQ=255)                                                                                                 tGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGcgttt                                   >  2:753898/1‑140 (MQ=255)                                                                                                  ggTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTa                                           <  1:431466/131‑1 (MQ=255)                                                                                                  ggTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTa                                           >  2:431466/1‑131 (MQ=255)                                                                                                  ggTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGcgttta                                  >  2:262418/1‑140 (MQ=255)                                                                                                   gTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGcgtttac                                 <  2:163059/140‑1 (MQ=255)                                                                                                   gTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGcgtttac                                 <  1:590067/140‑1 (MQ=255)                                                                                                      aaaTCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGcgtttacgtt                              <  2:978719/140‑1 (MQ=255)                                                                                                        aTCGTCCAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGcgtttacgttta                            >  2:596292/1‑140 (MQ=255)                                                                                                             ccAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGcgtttac                                 >  2:966669/1‑130 (MQ=255)                                                                                                             ccAACGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGcgtttac                                 <  1:966669/130‑1 (MQ=255)                                                                                                                aCGTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATag                    <  2:1025187/140‑1 (MQ=255)                                                                                                                  gTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATagca                  <  2:52224/140‑1 (MQ=255)                                                                                                                  gTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATagca                  <  1:130863/140‑1 (MQ=255)                                                                                                                  gTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATagca                  <  1:924727/140‑1 (MQ=255)                                                                                                                  gTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATagca                  <  1:262418/140‑1 (MQ=255)                                                                                                                  gTGAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATagca                  <  1:387578/140‑1 (MQ=255)                                                                                                                    gAAAGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATagcagc                <  2:757812/140‑1 (MQ=255)                                                                                                                     aaaGTGACCACCGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGcc               <  1:111627/140‑1 (MQ=255)                                                                                                                           accaccGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATag                    <  1:821240/129‑1 (MQ=255)                                                                                                                           accaccGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATag                    >  2:821240/1‑129 (MQ=255)                                                                                                                           accaccGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGt         >  2:536225/1‑140 (MQ=255)                                                                                                                            ccaccGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTa        <  2:409273/140‑1 (MQ=255)                                                                                                                                cGAAAACGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGc    <  1:596292/140‑1 (MQ=255)                                                                                                                                  aaaaCGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATagcagc                >  1:513785/1‑126 (MQ=255)                                                                                                                                  aaaaCGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATagcagc                <  2:513785/126‑1 (MQ=255)                                                                                                                                  aaaaCGGCGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCt  >  2:637949/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     ACGAGATTCGTCAGGGCCAGCCGATTGGTCTGGGCGAAGCATCTAACGATACGTGGATCACCACCAAAGTGCGTTCGCAGCTCTTAACCAGCGACCTGGTGAAATCGTCCAACGTGAAAGTGACCACCGAAAACGGTGAAGTGTTCCTGATGGGGCTGGTGACTGAACGTGAAGCGAAAGCGGCGGCAGATATTGCCAGCCGGGTGAGCGGCGTGAAGCGGGTAACTACGGCGTTTACGTTTATTAAATAGCAGCCCCTTGTAATGCCT  >  NC_000913/3296734‑3297002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑