Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,409,250	(T)8→7	intergenic (+3/‑58)	fnrS → / → ynaL	small regulatory RNA FnrS/protein YnaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,409,243	0	T	.	100.0%	207.0 / NA	48	noncoding (115/119 nt)	fnrS	small regulatory RNA FnrS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (21/27);  total (21/27)

ATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCTGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGC  >  NC_000913/1409108‑1409374                                                                                                                                        |                                                                                                                                    aTTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCttttt                                                                                                                                 >  2:919136/1‑140 (MQ=255)  ttCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCtttttt                                                                                                                                >  1:690684/1‑140 (MQ=255)      gggCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCtttttttgcc                                                                                                                            <  2:693385/140‑4 (MQ=255)      gggCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCtttttttgcc                                                                                                                            <  1:550970/140‑4 (MQ=255)       ggCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCtttttttgcct                                                                                                                           >  2:240894/1‑136 (MQ=255)            tatCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGttat                                                                                                                     >  1:287083/1‑140 (MQ=255)            tatCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGttat                                                                                                                     >  1:4788/1‑140 (MQ=255)                 ctctTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATc                                                                                                                <  2:249340/140‑1 (MQ=255)                 ctctTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATc                                                                                                                >  1:561054/1‑140 (MQ=255)                       cAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTGCTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTc                                                                                                          <  2:1052074/140‑1 (MQ=255)                       cAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTc                                                                                                          <  2:563413/140‑1 (MQ=255)                       cAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTc                                                                                                          <  2:561054/140‑1 (MQ=255)                         ggTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTa                                                                                                        <  1:816809/140‑1 (MQ=255)                         ggTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTa                                                                                                        <  1:532517/140‑1 (MQ=255)                              aTGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGTGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCtt                                                                                                   >  2:953717/1‑140 (MQ=255)                               tGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCttt                                                                                                  >  2:512554/1‑140 (MQ=255)                               tGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCttt                                                                                                  >  1:322075/1‑140 (MQ=255)                                gCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTa                                                                                                 <  1:919136/140‑1 (MQ=255)                                  aaCGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAag                                                                                               <  1:495352/140‑1 (MQ=255)                                       cAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCATCTACCCTTTAAGAGTcc                                                                                          <  2:591949/140‑1 (MQ=255)                                         aGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTc                                                                                                          <  2:809973/122‑1 (MQ=255)                                         aGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTc                                                                                                          >  1:809973/1‑122 (MQ=255)                                           cGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCAccc                                                                                      >  1:246312/1‑140 (MQ=255)                                                ggCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGa                                                                                 >  2:950673/1‑140 (MQ=255)                                                 gCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGAc                                                                                <  1:512554/140‑1 (MQ=255)                                                  cGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGAcc                                                                               <  2:448962/140‑1 (MQ=255)                                                   gTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCa                                                                              <  1:950673/140‑1 (MQ=255)                                                   gTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCa                                                                              <  2:4788/140‑1 (MQ=255)                                                     tGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCaga                                                                            <  2:690684/140‑1 (MQ=255)                                                       cgcTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCGCCCAATGACCAGAgg                                                                          <  2:859302/140‑1 (MQ=255)                                                       cgcTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAgg                                                                          >  2:965667/1‑140 (MQ=255)                                                           ccATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGaaa                                                                      <  2:246312/140‑1 (MQ=255)                                                                 tGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATgac                                                                <  1:953717/140‑1 (MQ=255)                                                                      tACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAatat                                                                   >  1:459133/1‑132 (MQ=255)                                                                      tACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAatat                                                                   <  2:459133/132‑1 (MQ=255)                                                                        cTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACtt                                                         >  1:1010821/1‑140 (MQ=255)                                                                           ccTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACttatt                                                      >  1:135977/1‑140 (MQ=255)                                                                                tttGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTatttattt                                                 >  2:916800/1‑140 (MQ=255)                                                                                         cTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTcc                                        <  2:1070032/140‑1 (MQ=255)                                                                                          tGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCt                                       <  2:277462/140‑1 (MQ=255)                                                                                                 cacaATTGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCTGTCCCTg                                >  2:188349/1‑140 (MQ=255)                                                                                                      ttGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTcc                                        <  2:235797/127‑1 (MQ=255)                                                                                                      ttGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTcc                                        >  1:235797/1‑127 (MQ=255)                                                                                                      ttGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCTGTCCCTGAACCg                           >  2:370538/1‑140 (MQ=255)                                                                                                      ttGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCTGTCCCTGAACCg                           <  1:490300/140‑1 (MQ=255)                                                                                                      ttGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCTGTCCCTGAACCg                           >  1:422281/1‑140 (MQ=255)                                                                                                       tGATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCTGTCCCTGAACCGa                          <  2:135977/140‑1 (MQ=255)                                                                                                        gATTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCTGTCCCTGAACCGAt                         >  2:619554/1‑140 (MQ=255)                                                                                                         aTTCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCTGTCCCTGAACCGAtt                        >  1:439201/1‑140 (MQ=255)                                                                                                           tCGTACGACGCCGACTTTGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCTGTCCCTGAACCGATTcc                      >  1:191634/1‑140 (MQ=255)                                                                                                                          tttGATGAGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCTGTCCCTGAACCGATTCCTGGCGATCCTGTTcc       <  1:619554/140‑1 (MQ=255)                                                                                                                               tgaGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCTGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGc  <  2:287083/140‑1 (MQ=255)                                                                                                                               tgaGTCGGC‑TTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCTGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGc  <  1:604643/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    ATTCAGGGCAATATCTCTCTTGCAGGTGAATGCAACGTCAAGCGATGGGCGTTGCGCTCCATATTGTCTTACTTCCTTTTTTGAATTACTGCATAGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCTGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGC  >  NC_000913/1409108‑1409374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑