Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	141,576	Δ1 bp	coding (146/537 nt)	hpt →	hypoxanthine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	141,576	0	T	.	100.0%	309.4 / NA	71	coding (146/537 nt)	hpt	hypoxanthine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (40/31);  total (40/31)

GTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTATGGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGATGAAGATATCCGTGGCAAGG  >  NC_000913/141443‑141710                                                                                                                                      |                                                                                                                                       gTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGAc                                                                                                                                 >  1:529488/1‑140 (MQ=255)      aGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCtgtg                                                                                                                            <  1:724464/140‑1 (MQ=255)       gTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGc                                                                                                                           >  1:426401/1‑140 (MQ=255)          aTGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGt                                                                                                                        >  2:381940/1‑140 (MQ=255)          aTGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGt                                                                                                                        >  2:270046/1‑140 (MQ=255)          aTGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGt                                                                                                                        >  1:807823/1‑140 (MQ=255)           tGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTg                                                                                                                       <  1:777690/140‑1 (MQ=255)            gttCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGa                                                                                                                      >  2:398623/3‑140 (MQ=255)            gATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGa                                                                                                                      >  1:636666/1‑140 (MQ=255)              tCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAg                                                                                                                    <  2:426401/140‑1 (MQ=255)              tCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAg                                                                                                                    <  2:939774/140‑1 (MQ=255)               ccccGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGGCGTCAGATTACTGAGCGTTACAAAGACAGCTGCAGCGATATGGAGCTGGTGGGTGTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGGAgc                                                                                                                   >  2:182294/1‑139 (MQ=255)                cccGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGtt                                                                                                                  >  1:857657/1‑140 (MQ=255)                  cGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGTGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCa                                                                                                                >  1:907667/1‑140 (MQ=255)                  cGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCa                                                                                                                >  1:549071/1‑140 (MQ=255)                    aaGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAgg                                                                                                              >  2:292451/1‑140 (MQ=255)                     aGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGt                                                                                                             <  1:227147/140‑1 (MQ=255)                         gagaTTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTc                                                                                                                 <  2:122849/132‑1 (MQ=255)                         gagaTTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTc                                                                                                                 >  1:122849/1‑132 (MQ=255)                                 aGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGt                                                                                                 >  1:535553/1‑140 (MQ=255)                                  gcgcGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTc                                                                                                <  1:381940/140‑1 (MQ=255)                                      gTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACt                                                                                            >  2:705670/1‑140 (MQ=255)                                      gTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACt                                                                                            >  1:960638/1‑140 (MQ=255)                                      gTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACt                                                                                            >  1:816700/1‑140 (MQ=255)                                                    ggTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTcc                                                                              >  1:371935/1‑140 (MQ=255)                                                         tcAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTa                                                                         <  2:857657/140‑1 (MQ=255)                                                              ttACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTa                                                                    <  2:906802/140‑1 (MQ=255)                                                              ttACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTa                                                                    <  2:529488/140‑1 (MQ=255)                                                              ttACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTa                                                                    <  2:929487/140‑1 (MQ=255)                                                                 cTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCg                                                                 <  1:705670/140‑1 (MQ=255)                                                                   gAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGc                                                               <  1:292451/140‑1 (MQ=255)                                                                    ggCGTTACAAAAACAGCGGCAGCGATAGGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACTTGTGCCGTGAAGTTCAGGTATCCCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCa                                                              <  2:535553/139‑1 (MQ=255)                                                                    aGCGTTACAAAGACAGCGGCAGCGATATGGTGTTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCa                                                              <  1:270046/140‑1 (MQ=255)                                                                      cGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATg                                                            >  2:116739/1‑140 (MQ=255)                                                                       gTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGt                                                           >  2:542951/1‑140 (MQ=255)                                                                          aCAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTcca                                                        <  1:780256/140‑1 (MQ=255)                                                                                aCAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTc                                                          >  1:714497/1‑132 (MQ=255)                                                                                aCAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTc                                                          <  2:714497/132‑1 (MQ=255)                                                                                aCAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCAccc                                                  >  2:984519/1‑140 (MQ=255)                                                                                  aGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGt                                                >  1:1028602/1‑140 (MQ=255)                                                                                  aGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGt                                                >  2:490279/1‑140 (MQ=255)                                                                                   gCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTg                                               >  1:867173/1‑140 (MQ=255)                                                                                   gCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTg                                               >  2:882539/1‑140 (MQ=255)                                                                                   gCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTg                                               >  2:311846/1‑140 (MQ=255)                                                                                   gCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTg                                               >  2:41546/1‑140 (MQ=255)                                                                                   gCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTg                                               >  1:155810/1‑140 (MQ=255)                                                                                   gCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTg                                               >  2:413766/1‑140 (MQ=255)                                                                                     ggCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGAt                                             >  2:422493/1‑140 (MQ=255)                                                                                           gATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGaaa                                       <  2:119529/140‑1 (MQ=255)                                                                                           gATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGaaa                                       <  2:1028602/140‑1 (MQ=255)                                                                                                  tGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCa                                <  1:984519/140‑1 (MQ=255)                                                                                                   gCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCaa                               <  1:490279/140‑1 (MQ=255)                                                                                                    cTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCaaa                              >  2:469890/1‑140 (MQ=255)                                                                                                      ggtggGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGa                            <  2:549071/140‑1 (MQ=255)                                                                                                       gtggGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGAtgtg                                          >  1:53790/1‑125 (MQ=255)                                                                                                       gtggGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGAtgtg                                          <  2:53790/125‑1 (MQ=255)                                                                                                       gtggGTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGAt                           >  2:867121/1‑140 (MQ=255)                                                                                                           gTCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTgg                       >  1:167720/1‑140 (MQ=255)                                                                                                            tCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGa                      >  1:832521/1‑140 (MQ=255)                                                                                                            tCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGa                      <  2:155810/140‑1 (MQ=255)                                                                                                            tCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGa                      >  2:9693/1‑140 (MQ=255)                                                                                                            tCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGa                      <  1:469890/140‑1 (MQ=255)                                                                                                            tCTGCTGCGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGGGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGa                      <  1:155911/140‑1 (MQ=255)                                                                                                             ctgctgCGTGGCTCATTTATGTTTA‑GGCGGACTTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGAt                     <  2:867173/140‑1 (MQ=255)                                                                                                              tgctgcGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGATg                    >  2:626667/1‑140 (MQ=255)                                                                                                                  gcGTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGATGAAGa                <  2:807823/140‑1 (MQ=255)                                                                                                                    gTGGCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATTCTCAAAAATCTCGATGAAGata              >  2:155911/1‑140 (MQ=255)                                                                                                                       gCTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGATGAAGATATcc           <  2:371935/140‑1 (MQ=255)                                                                                                                        cTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGATGAAGATATCCg          <  2:816700/140‑1 (MQ=255)                                                                                                                        cTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGATGAAGATATCCg          <  2:960638/140‑1 (MQ=255)                                                                                                                        cTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGATGAAGATATCCg          <  1:116739/140‑1 (MQ=255)                                                                                                                        cTCATTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCGCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGATGAAGATATCCg          >  1:672311/1‑140 (MQ=255)                                                                                                                           aTTTATGTTTA‑GGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGATGAAGATATCCGTgg       >  2:601599/1‑140 (MQ=255)                                                                                                                               atgttta‑ggCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGATGAAGATATCCGTGGCAAg   <  1:413766/140‑1 (MQ=255)                                                                                                                                tgttta‑ggCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGATGAAGATATCCGTGGCAAgg  <  1:208008/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       GTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTATGGCGGACCTGTGCCGTGAAGTTCAGGTATCTCATGAAGTCGACTTTATGACCGCCTCCAGCTACGGTAGCGGCATGTCCACCACCCGTGATGTGAAAATCCTCAAAGATCTGGATGAAGATATCCGTGGCAAGG  >  NC_000913/141443‑141710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑