| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,293,106 | (G)7→8 | coding (1595/1659 nt) | nrfE → | putative cytochrome c‑type biogenesis protein NrfE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,293,099 | 1 | . | G | 94.2% | 179.0 / 3.1 | 52 | coding (1588/1659 nt) | nrfE | putative cytochrome c‑type biogenesis protein NrfE |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (23/26); total (24/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
AAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAG > NC_000913/4292962‑4293219 | aaGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑gg > 2:229845/1‑140 (MQ=255) aGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑ggg > 2:42671/1‑140 (MQ=255) gCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑gggg > 1:410834/1‑140 (MQ=255) cACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑ggggg > 2:370383/1‑140 (MQ=255) cACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑ggggg > 2:462815/1‑140 (MQ=255) cGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑ggggggg > 2:234922/1‑140 (MQ=255) cGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑ggggggggag < 2:799286/140‑4 (MQ=255) gTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑ggggggggagg < 1:531055/140‑5 (MQ=255) tCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑ggggggggagga < 2:370404/140‑6 (MQ=255) tgatgGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑ggggggggagga < 2:419769/132‑6 (MQ=255) tgatgGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑ggggggggagga > 1:419769/1‑127 (MQ=255) gatgGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTtgatg < 1:234922/140‑1 (MQ=255) aCCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCg > 2:353893/1‑140 (MQ=255) ccGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCgg < 1:462815/140‑1 (MQ=255) ccGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCgg < 1:429209/140‑1 (MQ=255) tCAATTCGCTGGAACGGCATCCATGACTGGTACGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGgcg < 2:952456/140‑1 (MQ=255) cAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGGAGGATTGTTGATGATTGCGGGCGCa < 1:42671/140‑1 (MQ=255) aaTTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCa < 1:353893/140‑1 (MQ=255) ggCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGAt < 2:410834/140‑1 (MQ=255) gCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATg < 1:763570/140‑1 (MQ=255) tCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCg > 2:267523/1‑140 (MQ=255) tCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCg > 1:295644/1‑140 (MQ=255) aCTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGgaa > 2:203130/1‑140 (MQ=255) cTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGgaag < 2:956818/140‑1 (MQ=255) gTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGaagaag > 1:1005077/1‑140 (MQ=255) tATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAgc > 2:952018/1‑140 (MQ=255) tATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAgc > 1:552287/1‑140 (MQ=255) tGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAgcgc > 1:380294/1‑140 (MQ=255) gCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGcgcg < 1:952018/140‑1 (MQ=255) gCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGGGGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGa > 2:919814/1‑140 (MQ=255) cGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGa < 2:295644/140‑1 (MQ=255) ggTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGAt < 1:203130/140‑1 (MQ=255) ggTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGAt < 2:552287/140‑1 (MQ=255) aTGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAAt > 1:725968/1‑140 (MQ=255) aTGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAAt > 1:618461/1‑140 (MQ=255) aTGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAAt > 2:445737/1‑140 (MQ=255) aTGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAAt > 1:435390/1‑140 (MQ=255) ggAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAgg < 2:913412/140‑1 (MQ=255) gagaAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAggg < 2:426050/140‑1 (MQ=255) gagaAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAggg < 1:644464/140‑1 (MQ=255) gaAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCt < 1:666633/140‑1 (MQ=255) gaAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCt > 2:68740/1‑140 (MQ=255) gaAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCt > 2:666633/1‑140 (MQ=255) gaAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCt > 1:30967/1‑140 (MQ=255) cTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCa < 2:618461/140‑1 (MQ=255) gCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCt < 2:30967/140‑1 (MQ=255) gCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTa < 2:552799/140‑1 (MQ=255) ccGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTa < 1:87892/140‑1 (MQ=255) ccGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTa < 1:445737/140‑1 (MQ=255) gATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTActg > 1:507106/1‑140 (MQ=255) tatgtaCAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTgcg < 2:380294/140‑1 (MQ=255) tatgtaCAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTgcg < 2:725968/140‑1 (MQ=255) tgtaCAAAGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCa > 2:690960/1‑140 (MQ=255) aaaGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTc < 1:70823/140‑1 (MQ=255) aGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGttt < 1:257757/127‑1 (MQ=255) aGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGttt > 2:257757/1‑127 (MQ=255) aGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAg > 2:671348/1‑140 (MQ=255) aGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAg > 1:564062/1‑140 (MQ=255) aGCGGTGTGCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAAAAGCGCGATGAATAAAGGGCTTCTCACGTTACTCCGGTTATTTACCTGTTTTGCGCACGCTCAg > 2:371701/1‑140 (MQ=255) tgtgCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTgcg < 2:160082/126‑1 (MQ=255) tgtgCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTgcg > 1:160082/1‑126 (MQ=255) tgCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTgcg > 1:371515/1‑124 (MQ=255) tgCGCTGGATCTGGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTgcg < 2:371515/124‑1 (MQ=255) | AAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAG > NC_000913/4292962‑4293219 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |