Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	868,422	(C)8→9	coding (903/1872 nt)	gsiA →	glutathione ABC transporter ATP binding subunit GsiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	868,414	1	.	C	100.0%	143.3 / NA	41	A299P (GCC→CCC)	gsiA	glutathione ABC transporter ATP binding subunit GsiA
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (16/25);  total (16/25)

CGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCGTTGAGAAAGT  >  NC_000913/868275‑868547                                                                                                                                             |                                                                                                                                      cGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAgg                                                                                                                                        <  1:531625/140‑1 (MQ=255)   tCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑cc                                                                                                                                     <  2:411251/140‑1 (MQ=255)           aTTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑ccccccccca                                                                                                                             >  2:375508/1‑138 (MQ=255)                       ccGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGaaaa                                                                                                                  <  1:645857/140‑1 (MQ=255)                               ggcTTACTCCCGTGCGCTGTTAGCTGCTGGTCCGCAACTTTGAGCGATGAAAGGGTTAGATTAACCCCAACGTTTCCCGGTGATATCGGTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGGAGAAAACGgtggtg                                                                                                          <  2:165805/138‑1 (MQ=255)                                     cacCCCTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGc                                                                                                    >  1:347492/1‑140 (MQ=255)                                      acCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCg                                                                                                   <  2:279565/140‑1 (MQ=255)                                      acCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCg                                                                                                   <  2:574313/140‑1 (MQ=255)                                        ccGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGaa                                                                                                 <  2:897838/140‑1 (MQ=255)                                        ccGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGaa                                                                                                 <  1:18471/140‑1 (MQ=255)                                          gTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAcc                                                                                               >  1:247773/1‑140 (MQ=255)                                          gTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAcc                                                                                               <  1:170456/140‑1 (MQ=255)                                          gTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAcc                                                                                               <  1:590050/140‑1 (MQ=255)                                          gTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAcc                                                                                               >  2:162458/1‑140 (MQ=255)                                          gTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAcc                                                                                               >  2:1026200/1‑140 (MQ=255)                                           tGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGCTGGCGAAccc                                                                                              >  1:165805/1‑139 (MQ=255)                                           tGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCt                                                                                              >  1:579826/1‑140 (MQ=255)                                                  ttAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTAc                                                                                       >  2:736016/1‑140 (MQ=255)                                                  ttAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTAc                                                                                       <  1:1026200/140‑1 (MQ=255)                                                      ctgctgTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGt                                                                                   <  1:375508/140‑1 (MQ=255)                                                           gTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTa                                                                              <  1:102037/140‑1 (MQ=255)                                                           gTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTa                                                                              <  1:625256/140‑1 (MQ=255)                                                            ttCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAcc                                                                                               <  2:600598/122‑1 (MQ=255)                                                            ttCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAAcc                                                                                               >  1:600598/1‑122 (MQ=255)                                                            ttCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTaa                                                                             >  2:267783/1‑140 (MQ=255)                                                                      tGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCAcc                                                                   >  2:671185/1‑140 (MQ=255)                                                                      tGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCAcc                                                                   <  1:860676/140‑1 (MQ=255)                                                                        gTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCg                                                                 >  2:207346/1‑140 (MQ=255)                                                                         tGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGGACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGt                                                                >  2:811836/1‑140 (MQ=255)                                                                                         gATTCTCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCCCCCGTTTCACTTTGCGCAGCg                                                <  2:247773/140‑1 (MQ=255)                                                                                         gATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCg                                                <  1:267783/140‑1 (MQ=255)                                                                                         gATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCg                                                <  2:747962/140‑1 (MQ=255)                                                                                         gATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCg                                                <  2:739109/140‑1 (MQ=255)                                                                                               ccccGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTttgt                                          >  1:884753/1‑140 (MQ=255)                                                                                                   gACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCg                                                <  1:597108/130‑1 (MQ=255)                                                                                                   gACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCg                                                >  2:597108/1‑130 (MQ=255)                                                                                                   gACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGaa                                      <  1:207346/140‑1 (MQ=255)                                                                                                         tCCCGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGt                                <  1:221694/140‑1 (MQ=255)                                                                                                           ccGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTaa                              <  1:736016/140‑1 (MQ=255)                                                                                                            cGTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAAc                             <  1:162458/140‑1 (MQ=255)                                                                                                             gTTGATATCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAAcg                            <  2:347492/140‑1 (MQ=255)                                                                                                                 atatCGCTTGAACATCCAGCGAACCAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCggg                        <  1:215706/140‑1 (MQ=255)                                                                                                                 atatCCCTTGAACATCCAACGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCggg                        <  1:795560/140‑1 (MQ=255)                                                                                                                   atCGCTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGaa                      >  1:811626/1‑140 (MQ=255)                                                                                                                       cTTGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGc                  >  2:289002/1‑140 (MQ=255)                                                                                                                        ttGAACATCCAGCGAAACAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCa                 <  1:671185/140‑1 (MQ=255)                                                                                                                                       aaCAGGCCCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCGTTGAGAAAGt  <  1:811836/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                      CGTCGAACAGATTTTTCATGCACCGCAACATCCTTACACCCGTGCGCTGTTAGCTGCTGTTCCGCAACTTGGTGCGATGAAAGGGTTAGATTATCCCCGACGTTTCCCGTTGATATCGCTTGAACATCCAGCGAAACAGG‑CCCCCCCCATCGAGCAGAAAACGGTGGTGGATGGCGAACCTGTTTTACGAGTGCGTAATCTTGTCACCCGTTTCCCTTTGCGCAGCGGTTTGTTGAATCGCGTAACGCGGGAAGTGCATGCCGTTGAGAAAGT  >  NC_000913/868275‑868547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑