Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,097,580	(G)7→8	noncoding (73/88 nt)	serX ←	tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,573	1	.	G	93.3%	44.4 / ‑2.7	15	noncoding (80/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (0/14);  total (0/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

CTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAA‑CCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAA  >  NC_000913/1097451‑1097697                                                                                                                            |                                                                                                                              cTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAA‑cc                                                                                                              <  1:1056081/140‑1 (MQ=18)             cTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCCGATACGtt                                                                                                   <  1:1060134/140‑1 (MQ=255)             cTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCCGATACGtt                                                                                                   <  1:836118/140‑1 (MQ=255)              tGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCCGATACGTTg                                                                                                  <  1:557579/140‑1 (MQ=255)               gTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCCGATACGTTGc                                                                                                 <  1:253505/140‑1 (MQ=255)                       ttGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCCGATACGTTGCCGTATaca                                                                                         <  1:971665/140‑1 (MQ=255)                         gTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCCGATACGTTGCCGTATacaca                                                                                       <  1:653531/140‑1 (MQ=255)                                              ctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCa                                                                  <  1:1049931/140‑1 (MQ=255)                                                                       aCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTg                                         <  1:559675/140‑1 (MQ=255)                                                                        cGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGt                                        <  1:637122/140‑1 (MQ=255)                                                                               gagacaggTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTg                                <  1:1035447/133‑1 (MQ=255)                                                                                         cTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTgg                       <  1:564386/140‑1 (MQ=255)                                                                                             cGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACg                   <  1:440985/140‑1 (MQ=255)                                                                                                 cGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGAAA‑CCCCCCCCACGTTTCCCTATACACACCTTTCCGGCGGGCTCCTTCCGCCACTCCGGCACCCCCCCCAAATTTCGTTCCTGTCTTTCTGGGActgtctc              >  2:1035447/1‑134 (MQ=9)                                                                                                      tcttctTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAAt          <  1:806906/140‑1 (MQ=255)                                                                                                              aaaTATGGCGGTGAGGGGGGGGATTCGAACCCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGaa  <  1:743353/140‑1 (MQ=255)                                                                                                                            |                                                                                                                              CTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAA‑CCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAA  >  NC_000913/1097451‑1097697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑