Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,828,111	T→C	K185K (AAA→AAG)	gltS ←	glutamate:sodium symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,828,111	0	T	C	100.0%	205.5 / NA	57	K185K (AAA→AAG)	gltS	glutamate:sodium symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/24);  total (33/24)

CGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGC  >  NC_000913/3827979‑3828244                                                                                                                                     |                                                                                                                                      cGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCa                                                                                                                                >  2:97464/1‑140 (MQ=255)     aGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATa                                                                                                                            >  2:377877/1‑140 (MQ=255)     aGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATa                                                                                                                            >  2:218578/1‑140 (MQ=255)     aGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATa                                                                                                                            >  2:113316/1‑140 (MQ=255)        cAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAgcg                                                                                                                         <  2:710490/140‑1 (MQ=255)             aGCAATCAGCGCGATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCa                                                                                                                    >  2:404440/1‑140 (MQ=255)             aGCAATCAGCGCGATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCa                                                                                                                    >  2:659455/1‑140 (MQ=255)             aGCAATCAGCGCGATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCa                                                                                                                    >  1:383033/1‑140 (MQ=255)                     gcgcGATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCg                                                                                                            >  1:423732/1‑140 (MQ=255)                      cgcgATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGc                                                                                                           >  2:651766/1‑140 (MQ=255)                       gcgATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGcc                                                                                                          <  2:949669/140‑1 (MQ=255)                       gcgATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGcc                                                                                                          >  2:772796/1‑140 (MQ=255)                                   aTCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAgccgcc                                                                                              <  2:383033/140‑1 (MQ=255)                                   aTCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAgccgcc                                                                                              >  2:542921/1‑140 (MQ=255)                                   aTCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAgccgcc                                                                                              <  1:635971/140‑1 (MQ=255)                                        cACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCa                                                                                         >  1:900442/1‑140 (MQ=255)                                        cACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCa                                                                                         >  2:740892/1‑140 (MQ=255)                                        cACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCa                                                                                         >  2:242138/1‑140 (MQ=255)                                                 ggTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGa                                                                                <  1:242138/140‑1 (MQ=255)                                                  gTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGaa                                                                               <  1:123627/140‑1 (MQ=255)                                                    gATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACg                                                                             <  1:404440/140‑1 (MQ=255)                                                         tGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCTAGCACCAGACCGAACGTTGca                                                                        <  1:218270/140‑1 (MQ=255)                                                            gTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAg                                                                     >  1:403881/1‑140 (MQ=255)                                                             tCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAgg                                                                    >  2:892765/1‑140 (MQ=255)                                                               ccACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGAcc                                                                                  >  2:243269/1‑124 (MQ=255)                                                               ccACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGAcc                                                                                  <  1:243269/124‑1 (MQ=255)                                                               ccACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGcc                                                                  <  1:97464/140‑1 (MQ=255)                                                               ccACATCCGGCTCTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGcc                                                                  >  2:704647/1‑140 (MQ=255)                                                                   aTCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCg                                                              >  1:929990/1‑140 (MQ=255)                                                                      cGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCa                                                           <  2:900442/140‑1 (MQ=255)                                                                        gCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACt                                                         >  1:17529/1‑140 (MQ=255)                                                                               aaaCGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCg                                                  >  2:109154/1‑140 (MQ=255)                                                                                 cccccGTCGGGACTTCCCGGTCCTCCGGAATACCCTTCGGGGTGGTGGAGTGCTCCACTAGAAACCGCGCAACCGGACCGCCAATCAAGCCGCTCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCa                                                <  2:943466/137‑1 (MQ=255)                                                                                 aCGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCa                                                <  1:740892/140‑1 (MQ=255)                                                                                 aCGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCa                                                >  1:130243/1‑140 (MQ=255)                                                                                      gTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGt                                           <  2:130243/140‑1 (MQ=255)                                                                                       tCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTg                                          <  1:833579/140‑1 (MQ=255)                                                                                       tCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTg                                          <  1:377877/140‑1 (MQ=255)                                                                                       tCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTg                                          <  1:218578/140‑1 (MQ=255)                                                                                                       aTCCGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCaa                          >  1:796248/1‑140 (MQ=255)                                                                                                         ccGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATg                        <  2:17529/140‑1 (MQ=255)                                                                                                          cGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGa                       <  2:450791/140‑1 (MQ=255)                                                                                                          cGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGa                       <  1:113316/140‑1 (MQ=255)                                                                                                          cGGAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGa                       >  1:824600/1‑140 (MQ=255)                                                                                                           ggAATACCGTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGaa                      <  1:892765/140‑1 (MQ=255)                                                                                                                   gTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTAc              >  1:684002/1‑140 (MQ=255)                                                                                                                   gTTCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTAc              >  1:436148/1‑140 (MQ=255)                                                                                                                    ttCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGa                       <  1:912736/130‑1 (MQ=255)                                                                                                                    ttCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGa                       >  2:912736/1‑130 (MQ=255)                                                                                                                    ttCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACt             >  2:283295/1‑140 (MQ=255)                                                                                                                    ttCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACt             >  2:198299/1‑140 (MQ=255)                                                                                                                    ttCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACt             >  1:763927/1‑140 (MQ=255)                                                                                                                    ttCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACt             >  1:617067/1‑140 (MQ=255)                                                                                                                     tCGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTc            <  1:109154/140‑1 (MQ=255)                                                                                                                      cGGCGTGGTGGAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTcc           >  2:1054858/1‑140 (MQ=255)                                                                                                                            ggtggAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAATGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAg     <  2:689344/140‑1 (MQ=255)                                                                                                                             gtggAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAgc    >  2:164674/1‑140 (MQ=255)                                                                                                                               ggAGTGCTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAgcgc  <  1:164674/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      CGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGC  >  NC_000913/3827979‑3828244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑