Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,847,243	T→C	intergenic (‑76/+62)	uhpT ← / ← uhpC	hexose‑6‑phosphate:phosphate antiporter/inner membrane protein sensing glucose‑6‑phosphate

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,847,243	0	T	C	100.0%	300.7 / NA	81	intergenic (‑76/+62)	uhpT/uhpC	hexose‑6‑phosphate:phosphate antiporter/inner membrane protein sensing glucose‑6‑phosphate
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/40);  total (41/40)

AGCGGAAGGTCCAGGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGAT  >  NC_000913/3847118‑3847379                                                                                                                              |                                                                                                                                         aGCGGAAGGTCCAGGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTg                                                                                                                            <  1:334244/140‑1 (MQ=255)     gAAGGTCCAGGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGaaa                                                                                                                        >  1:30655/1‑140 (MQ=255)               ggTCGGCTTGCGAACTTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGt                                                                                                              <  2:437546/140‑1 (MQ=255)                    gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGc                                                                                                         >  1:1017345/1‑140 (MQ=255)                    gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGc                                                                                                         >  1:1033247/1‑140 (MQ=255)                    gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGc                                                                                                         >  2:590267/1‑140 (MQ=255)                    gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGc                                                                                                         >  2:926536/1‑140 (MQ=255)                    gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGc                                                                                                         >  1:13890/1‑140 (MQ=255)                    gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGc                                                                                                         >  2:764321/1‑140 (MQ=255)                    gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGc                                                                                                         >  2:810442/1‑140 (MQ=255)                    gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGc                                                                                                         >  1:926848/1‑140 (MQ=255)                    gCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGc                                                                                                         >  2:210601/1‑140 (MQ=255)                       tGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCgg                                                                                                      <  2:13890/140‑1 (MQ=255)                                 gTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTg                                                                                            <  1:1014830/140‑1 (MQ=255)                                  tttAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATg                                                                                                 <  2:157444/134‑1 (MQ=255)                                  tttAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATg                                                                                                 >  1:157444/1‑134 (MQ=255)                                   ttAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGaa                                                                                          <  1:290025/140‑1 (MQ=255)                                   ttAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGaa                                                                                          <  1:204051/140‑1 (MQ=255)                                    tAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGaaa                                                                                         >  1:486542/1‑140 (MQ=255)                                          aGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGaa                                                                                               >  1:365600/1‑128 (MQ=255)                                          aGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGaa                                                                                               <  2:365600/128‑1 (MQ=255)                                           gCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTgag                                                                                  >  1:460215/1‑140 (MQ=255)                                           gCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATACGAAGTGAAAAGGTgag                                                                                  >  1:1070649/1‑140 (MQ=255)                                            ccAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTgaga                                                                                 >  1:827348/1‑140 (MQ=255)                                            ccAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTgaga                                                                                 >  1:476430/1‑140 (MQ=255)                                             cAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGAt                                                                                <  2:1041636/140‑1 (MQ=255)                                                 aTGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCAt                                                                            <  1:926536/140‑1 (MQ=255)                                                 aTGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCAt                                                                            <  1:764321/140‑1 (MQ=255)                                                    ggTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCAc                                                                         <  1:12992/140‑1 (MQ=255)                                                     gTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACg                                                                        >  1:223945/1‑140 (MQ=255)                                                     gTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACg                                                                        >  1:761559/1‑140 (MQ=255)                                                         cTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGAAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCTGATATGAAGTGAAAAGGTGAGATGCATCACGCTTc                                                                    >  2:743316/1‑140 (MQ=255)                                                          tCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcg                                                                   >  2:1070329/1‑140 (MQ=255)                                                            cTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                 <  2:486542/140‑1 (MQ=255)                                                            cTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                 <  2:367081/140‑1 (MQ=255)                                                            cTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                 <  1:1070329/140‑1 (MQ=255)                                                            cTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                 <  2:926848/140‑1 (MQ=255)                                                            cTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                 <  2:827348/140‑1 (MQ=255)                                                            cTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATACGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                 <  2:1070649/140‑1 (MQ=255)                                                              gAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcgcg                                                               <  1:284420/140‑1 (MQ=255)                                                              gAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGACAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcgcg                                                               <  1:583914/140‑1 (MQ=255)                                                               aaaTGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                 <  1:250114/137‑1 (MQ=255)                                                               aaaTGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                 >  2:250114/1‑137 (MQ=255)                                                                   gAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTc                                                          >  2:576859/1‑140 (MQ=255)                                                                    aaTACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCt                                                         >  2:887675/1‑140 (MQ=255)                                                                    aaTACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCt                                                         >  1:976038/1‑140 (MQ=255)                                                                      tACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTgg                                                       >  1:668237/1‑140 (MQ=255)                                                                         cTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcgcg                                                               <  2:836545/129‑1 (MQ=255)                                                                         cTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcgcg                                                               >  1:836545/1‑129 (MQ=255)                                                                           gCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                 <  1:228259/125‑1 (MQ=255)                                                                           gCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                 >  2:228259/1‑125 (MQ=255)                                                                            cGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                 <  1:649117/124‑1 (MQ=255)                                                                            cGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                 >  2:649117/1‑124 (MQ=255)                                                                            cGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                 <  1:843153/124‑1 (MQ=255)                                                                            cGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTcgcg                                                                 >  2:843153/1‑124 (MQ=255)                                                                              tGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCaa                                               >  2:558543/1‑140 (MQ=255)                                                                               gAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCaaa                                              <  2:30655/140‑1 (MQ=255)                                                                                aGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCaaaa                                             >  2:563541/1‑140 (MQ=255)                                                                                  gcatgcatTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAg                                           <  1:563541/140‑1 (MQ=255)                                                                                  gcatgcatTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAg                                           <  2:761559/140‑1 (MQ=255)                                                                                   catgcatTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAgg                                          >  1:414064/1‑140 (MQ=255)                                                                                     tgcatTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGc                                        <  1:810442/140‑1 (MQ=255)                                                                                     tgcatTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGc                                        <  2:476430/140‑1 (MQ=255)                                                                                     tgcatTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGc                                        <  1:590267/140‑1 (MQ=255)                                                                                     tgcatTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGc                                        <  2:1017345/140‑1 (MQ=255)                                                                                      gcatTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCa                                       >  1:902537/1‑140 (MQ=255)                                                                                            tAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAgg                                          >  2:301773/1‑131 (MQ=255)                                                                                            tAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAgg                                          <  1:301773/131‑1 (MQ=255)                                                                                             aaaaaTCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAAcag                                <  1:576859/140‑1 (MQ=255)                                                                                               aaaTCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGGAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGACGTCTGGGCGTTCAAAAAGGGCAGTAAcagca                              >  2:952802/1‑140 (MQ=255)                                                                                                 aTCAGCAGCAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGt                            >  1:609884/1‑140 (MQ=255)                                                                                                    agcagcAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCg                         >  1:708051/1‑140 (MQ=255)                                                                                                    agcagcAAATCAGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCg                         >  1:380911/1‑140 (MQ=255)                                                                                                               aGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATTCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCcggc              <  1:952802/140‑1 (MQ=255)                                                                                                               aGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCcggc              <  2:708051/140‑1 (MQ=255)                                                                                                               aGGCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCcggc              <  2:668237/140‑1 (MQ=255)                                                                                                                 gCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCcggcgg            <  2:380911/140‑1 (MQ=255)                                                                                                                 gCCTGAGATAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCcggcgg            <  2:1033247/140‑1 (MQ=255)                                                                                                                      agaTAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGa                       <  2:1004898/124‑1 (MQ=255)                                                                                                                      agaTAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGa                       >  1:1004898/1‑124 (MQ=255)                                                                                                                      agaTAGCGCCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATa       >  2:867875/1‑140 (MQ=255)                                                                                                                           gcgcCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGAt  <  2:414064/140‑1 (MQ=255)                                                                                                                           gcgcCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGCGAGAAGGTGGGTTGCATCACGCTTCGCGCGGTTACTGGGCGTTCAAAAAAGTCAGTAGCAGCAATGCGGAAATCCCGGCGGCGATAGAAAt  >  2:386871/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                         AGCGGAAGGTCCAGGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGAT  >  NC_000913/3847118‑3847379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑