Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,966,740	A→G	E108E (GAA→GAG)	rho →	transcription termination factor Rho

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,966,740	0	A	G	100.0%	205.6 / NA	57	E108E (GAA→GAG)	rho	transcription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/26);  total (31/26)

CCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGG  >  NC_000913/3966608‑3966871                                                                                                                                     |                                                                                                                                    cctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTAtt                                                                                                                              >  2:435627/1‑140 (MQ=255)  ctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTAttt                                                                                                                             >  1:336146/1‑140 (MQ=255)    ccGTCCCGCAGACACCCCCTCCCCCGCGGGTCCTGATGACTTCTACTTTCCCCCTACCAAAACCCGCCGTTTCACCCCCCGCACGGGTGATCCCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTg                                                                                                                           <  2:582236/140‑1 (MQ=255)      ttcccGCAAACACCCCCCCCCCCGCCGTCCCTGATGACTTCTCCGTTCCCCCTACCAAAACCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTgcg                                                                                                                         <  2:3145/137‑1 (MQ=255)       ttCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTgcgc                                                                                                                        >  2:391346/1‑140 (MQ=255)                   cTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTa                                                                                                            >  1:419564/1‑140 (MQ=255)                     ccTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAAc                                                                                                          >  1:804401/1‑140 (MQ=255)                                    cTGATACCATCTATTTTCCCCCCACCAAAACCCCCGGTTTCACCCCCCGCACTGGTGATCCCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGAc                                                                                           <  2:804401/140‑1 (MQ=255)                                           cATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTg                                                                                    >  2:420840/1‑140 (MQ=255)                                              cTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaaa                                                                                 >  1:994204/1‑140 (MQ=255)                                                  gTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGc                                                                             >  2:329880/1‑140 (MQ=255)                                                      ccccTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGc                                                                         >  1:421434/1‑140 (MQ=255)                                                      ccccTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTCTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGc                                                                         >  1:894929/1‑140 (MQ=255)                                                                  cccgccgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCctc                                                             <  1:329880/139‑1 (MQ=255)                                                                     cccgTTTCAACCCCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCttt                                                          <  1:302242/139‑1 (MQ=255)                                                                       ggTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTga                                                        <  1:829229/139‑1 (MQ=255)                                                                       cgTTTCACCCCCCGCCCTGGTGATCCCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTga                                                        <  2:421434/140‑1 (MQ=255)                                                                       cgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTga                                                        >  2:145435/1‑140 (MQ=255)                                                                               ccTCCGCCCTGGTAATACCTTCTGGGTAAAGATGCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTaa                                                <  2:419564/140‑1 (MQ=255)                                                                                   cGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgag                                                       >  1:175830/1‑129 (MQ=255)                                                                                   cGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgag                                                       <  2:175830/129‑1 (MQ=255)                                                                                          gTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCAc                                     >  1:435030/1‑140 (MQ=255)                                                                                          gTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCAc                                     >  2:398685/1‑140 (MQ=255)                                                                                             aTACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCGCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa                                  >  2:742593/1‑140 (MQ=255)                                                                                             aTACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa                                  >  1:565679/1‑140 (MQ=255)                                                                                                   tctcTGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAActct                            <  1:145435/140‑1 (MQ=255)                                                                                                    ctctGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTg                                        >  1:445825/1‑127 (MQ=255)                                                                                                    ctctGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTg                                        <  2:445825/127‑1 (MQ=255)                                                                                                    ctctGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACtctc                           >  2:499375/1‑140 (MQ=255)                                                                                                    ctctGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACtctc                           >  2:329314/1‑140 (MQ=255)                                                                                                    ctctGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACtctc                           >  2:1057754/1‑140 (MQ=255)                                                                                                    ctctGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACtctc                           >  1:273361/1‑140 (MQ=255)                                                                                                    ctctGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACtctc                           >  1:996798/1‑140 (MQ=255)                                                                                                    ctctGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACtctc                           >  1:89480/1‑140 (MQ=255)                                                                                                    ctctGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACtctc                           >  2:985289/1‑140 (MQ=255)                                                                                                    ctctGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACtctc                           >  2:492787/1‑140 (MQ=255)                                                                                                     tctGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg                          <  2:273361/140‑1 (MQ=255)                                                                                                      ctGGTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGt                         <  1:742593/140‑1 (MQ=255)                                                                                                        ggaaaGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt                       <  1:535112/137‑1 (MQ=255)                                                                                                        ggTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt                       <  2:994204/140‑1 (MQ=255)                                                                                                        ggTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt                       <  1:435627/140‑1 (MQ=255)                                                                                                         gTAAGATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACGCCGCTGCACGCAAACTCTCGTCTg                      >  2:777545/1‑140 (MQ=255)                                                                                                             gcttCGTCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTa                  <  1:398685/138‑1 (MQ=255)                                                                                                             gATTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTa                  <  1:492787/140‑1 (MQ=255)                                                                                                              aTTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg                          >  1:587986/1‑131 (MQ=255)                                                                                                              aTTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg                          <  2:587986/131‑1 (MQ=255)                                                                                                              aTTCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTAt                 >  2:1060574/1‑140 (MQ=255)                                                                                                               ttCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGt                         <  2:367299/131‑1 (MQ=255)                                                                                                               ttCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGt                         >  1:367299/1‑131 (MQ=255)                                                                                                               ttCGCCCGCCGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATg                >  1:558577/1‑140 (MQ=255)                                                                                                                   cccgccGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt                       >  2:321448/1‑129 (MQ=255)                                                                                                                   cccgccGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt                       <  1:321448/129‑1 (MQ=255)                                                                                                                   cccgccGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAAc            <  2:1001180/140‑1 (MQ=255)                                                                                                                   cccgccGAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAAc            >  1:1001180/1‑140 (MQ=255)                                                                                                                    ccgccgAAAGAAGGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACg           <  2:558577/140‑1 (MQ=255)                                                                                                                             gaagGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACgg  <  2:133677/140‑1 (MQ=255)                                                                                                                             gaagGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACgg  <  1:329314/140‑1 (MQ=255)                                                                                                                             gaagGTGAGCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACgg  <  1:1057754/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    CCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGG  >  NC_000913/3966608‑3966871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑