Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	317,091	A→G	pseudogene (46/687 nt)	ykgA ←	putative DNA‑binding transcriptional regulator YkgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	317,091	0	A	G	100.0%	264.5 / NA	72	pseudogene (46/687 nt)	ykgA	putative DNA‑binding transcriptional regulator YkgA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/42);  total (30/42)

AAAGCCTTCGTTTGCGAATGTATTCTCCCAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCACTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTGGGCCCTAAGTGTATCAAAAATAGCAAGGGCGAAGGCAAAAG  >  NC_000913/316959‑317227                                                                                                                                     |                                                                                                                                         aaaGCCTTCGTTTGCGAATGTATTCTCCCAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATc                                                                                                                                   <  2:230353/140‑1 (MQ=255) aaaGCCTTCGTTTGCGAATGTATTCTCCCAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATc                                                                                                                                   <  2:877231/140‑1 (MQ=255) aaaGCCTTCGTTTGCGAATGTATTCTCCCAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATc                                                                                                                                   <  1:320337/140‑1 (MQ=255) aaaGCCTTCGTTTGCGAATGTATTCTCCCAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATc                                                                                                                                   <  1:439781/140‑1 (MQ=255) aaaGCCTTCGTTTGCGAATGTATTCTCCCAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATc                                                                                                                                   <  2:658775/140‑1 (MQ=255) aaaGCCTTCGTTTGCGAATGTATTCTCCCAAAGGCAAATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATc                                                                                                                                   <  2:900566/140‑1 (MQ=255)     ccTTCGTTTGCGAATGTATTCTCCCAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACt                                                                                                                               <  1:692041/140‑1 (MQ=255)        tCGTTTGCGAATGTATTCTCCCAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCa                                                                                                                            >  2:748094/1‑140 (MQ=255)          gTTTGCGAATGTATTCTCCCAAAGGCACATGCATGAAATGTCGGAACAGAAGCTGGATGTTGCGAGTGCTGTAGCCAGATTTCTGTGCGATAGCTTCGATTGAAATAGGGTGCACGAGATTGCGGTCAATCCACTCCAgg                                                                                                                          <  2:1049115/140‑1 (MQ=255)            ttGCGAATGTATTCTCCCAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAg                                                                                                                        <  2:151676/140‑1 (MQ=255)            ttGCGAATGTATTCTCCCAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAg                                                                                                                        <  1:766665/140‑1 (MQ=255)               cGAATGTATTCTCCCAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAgctg                                                                                                                     >  2:275780/1‑140 (MQ=255)                   tGTATTCTCCCAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGt                                                                                                                 >  2:50402/1‑140 (MQ=255)                     tATTCTCCCAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAg                                                                                                               <  1:913958/140‑1 (MQ=255)                     tATTCTCCCAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAg                                                                                                               <  1:374349/140‑1 (MQ=255)                        tctcCCAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAAt                                                                                                            >  2:785423/1‑140 (MQ=255)                            ccAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAAtcttc                                                                                                        >  2:595174/1‑140 (MQ=255)                             cAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAAtcttct                                                                                                       <  1:275780/140‑1 (MQ=255)                               aaGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGc                                                                                                     >  1:769751/1‑140 (MQ=255)                               aaGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGc                                                                                                     >  2:472498/1‑140 (MQ=255)                                   cacaTGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGa                                                                                                 <  1:748094/140‑1 (MQ=255)                                    acaTGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGat                                                                                                >  1:1008941/1‑140 (MQ=255)                                    acaTGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGat                                                                                                >  2:736234/1‑140 (MQ=255)                                      atgcatgAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGatca                                                                                              >  2:150633/1‑140 (MQ=255)                                      atgcatgAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGatca                                                                                              >  2:295261/1‑140 (MQ=255)                                       tgcatgAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGatcat                                                                                             <  2:262411/140‑1 (MQ=255)                                          atgAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCAtctc                                                                                          >  2:317868/1‑140 (MQ=255)                                           tgAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCa                                                                                         >  1:293809/1‑140 (MQ=255)                                            gAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCAt                                                                                        <  2:293809/140‑1 (MQ=255)                                             aaaTTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCAtt                                                                                       <  1:50402/140‑1 (MQ=255)                                             aaaTTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCAtt                                                                                       >  2:876917/1‑140 (MQ=255)                                                   cGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGa                                                                                                 >  2:481974/1‑124 (MQ=255)                                                   cGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGa                                                                                                 <  1:481974/124‑1 (MQ=255)                                                    ggAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATtc                                                                                      >  2:204630/1‑134 (MQ=255)                                                    ggAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATtc                                                                                      <  1:204630/134‑1 (MQ=255)                                                             gCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTg                                                                       <  1:472498/140‑1 (MQ=255)                                                             gCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTg                                                                       <  1:150633/140‑1 (MQ=255)                                                                     ttGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCg                                                               <  2:1008941/140‑1 (MQ=255)                                                                        cGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGca                                                             <  2:666880/139‑1 (MQ=255)                                                                        cGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGca                                                             >  1:666880/1‑139 (MQ=255)                                                                          tCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACAt                                                          <  2:941117/140‑1 (MQ=255)                                                                          tCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACAt                                                          <  1:295261/140‑1 (MQ=255)                                                                           ctgctgTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACAtt                                                         >  1:599434/1‑140 (MQ=255)                                                                           ctgctgTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACAtt                                                         >  1:784801/1‑140 (MQ=255)                                                                            tgctgTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACAttt                                                        <  2:769751/140‑1 (MQ=255)                                                                                 tAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGtt                                                   <  1:876917/140‑1 (MQ=255)                                                                                       gATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATAccc                                             <  1:645998/140‑1 (MQ=255)                                                                                       gATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATAccc                                             <  1:736234/140‑1 (MQ=255)                                                                                       gATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATAccc                                             <  1:705267/140‑1 (MQ=255)                                                                                       gATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATAccc                                             <  1:595174/140‑1 (MQ=255)                                                                                        aTTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCt                                            >  1:949524/1‑140 (MQ=255)                                                                                         tttCTGTGTGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTg                                           <  1:294551/140‑1 (MQ=255)                                                                                         tttCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTg                                           <  2:784801/140‑1 (MQ=255)                                                                                         tttCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTg                                           <  2:599434/140‑1 (MQ=255)                                                                                         tttCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTg                                           <  2:866853/140‑1 (MQ=255)                                                                                               tgCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTTCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTGGGCCCt                                     <  2:949524/140‑1 (MQ=255)                                                                                               tgCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTta                                                  >  1:987526/1‑127 (MQ=255)                                                                                               tgCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTta                                                  <  2:987526/127‑1 (MQ=255)                                                                                               tgCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTGGGCCCt                                     <  1:672328/140‑1 (MQ=255)                                                                                                  gATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATAccc                                             <  1:207262/129‑1 (MQ=255)                                                                                                  gATATCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATAccc                                             >  2:207262/1‑129 (MQ=255)                                                                                                    tatCTTCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTGGGCCCTAAgtg                                >  1:990215/1‑140 (MQ=255)                                                                                                         tCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTGGGCCCTAAGTGTAt                             <  2:876672/138‑1 (MQ=255)                                                                                                         tCGATTGAAATAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTGGGCCCTAAGTGTAt                             >  1:876672/1‑138 (MQ=255)                                                                                                                aaaTAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTGGGCCCTAAGTGTATCAAAAATAg                    >  2:769337/1‑140 (MQ=255)                                                                                                                aaaTAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTGGGCCCTAAGTGTATCAAAAATAg                    >  2:258812/1‑140 (MQ=255)                                                                                                                  aTAGGGTGCTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTGGGCCCTAAGTGTATCAAAAATAGCa                  <  2:990215/140‑1 (MQ=255)                                                                                                                          cTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTGGGCCCTAAGTGTATCAAAAATAGCAAGGGCGaa          >  1:695656/1‑140 (MQ=255)                                                                                                                          cTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTGGGCCCTAAGTGTATCAAAAATAGCAAGGGCGaa          >  1:474556/1‑140 (MQ=255)                                                                                                                          cTCAAGATTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTGGGCCCTAAGTGTATCAAAAATAGCAAGGGCGaa          >  2:927562/1‑140 (MQ=255)                                                                                                                                aTTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTGGGCCCTAAGTGTATCAAAAATAGCAAGGGCGAAGGCaaa    <  1:769337/140‑1 (MQ=255)                                                                                                                                aTTGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTGGGCCCTAAGTGTATCAAAAATAGCAAGGGCGAAGGCaaa    <  2:239915/140‑1 (MQ=255)                                                                                                                                  tGCGCTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTGGGCCCTAAGTGTATCAAAAATAGCAAGGGCGAAGGCAAAAg  >  1:756539/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                         AAAGCCTTCGTTTGCGAATGTATTCTCCCAAAGGCACATGCATGAAATTTCGGAACAGAAGCTGGATGTTGCGTCTGCTGTAGCCAGATTTCTGTGCGATATCTTCGATTGAAATAGGGTGCTCAAGATTGCACTCAATCCACTCCAGGAGCTGCTGTAGAATCTTCTGCCTGATCATCTCATTCTCCGTTAATTCTTTGTCATATCGCACATTTTCGTTATACCCTGGGCCCTAAGTGTATCAAAAATAGCAAGGGCGAAGGCAAAAG  >  NC_000913/316959‑317227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑