Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,313,658	C→T	N103N (AAC→AAT)	rpiB →	allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,313,658	0	C	T	100.0%	242.9 / NA	68	N103N (AAC→AAT)	rpiB	allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/34);  total (34/34)

AGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGA  >  NC_000913/4313522‑4313793                                                                                                                                         |                                                                                                                                        agGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAAtgtg                                                                                                                                      <  2:481648/140‑1 (MQ=255)    ttGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTg                                                                                                                                   <  1:842833/140‑1 (MQ=255)     tGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTgg                                                                                                                                  >  1:323096/1‑140 (MQ=255)         ggCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCttt                                                                                                                              <  2:323096/140‑1 (MQ=255)           cGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCttt                                                                                                                              >  1:261001/1‑138 (MQ=255)           cGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCttt                                                                                                                              <  2:261001/138‑1 (MQ=255)             ggATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGt                                                                                                                          >  1:342296/1‑140 (MQ=255)             ggATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGt                                                                                                                          >  1:981193/1‑140 (MQ=255)               aTTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTc                                                                                                                        <  1:216824/140‑1 (MQ=255)                   tGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGa                                                                                                                    <  1:20116/140‑1 (MQ=255)                       ttGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTgg                                                                                                                <  2:554072/140‑1 (MQ=255)                        tgtgGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGt                                                                                                               >  2:454666/1‑140 (MQ=255)                         gtgGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCttt                                                                                                                              >  2:679745/1‑124 (MQ=255)                         gtgGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCttt                                                                                                                              <  1:679745/124‑1 (MQ=255)                                    gTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACt                                                                                                   >  2:232118/1‑140 (MQ=255)                                       ggTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGc                                                                                                >  2:371287/1‑140 (MQ=255)                                       ggTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGc                                                                                                >  1:629072/1‑140 (MQ=255)                                       ggTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGc                                                                                                >  1:591469/1‑140 (MQ=255)                                                aTAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGAt                                                                                       >  2:996634/1‑140 (MQ=255)                                                 tAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGAtt                                                                                      >  1:660280/1‑140 (MQ=255)                                                   gcggcgAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATtgt                                                                                    >  1:61132/1‑140 (MQ=255)                                                      gcgAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGa                                                                                        <  1:1071733/133‑1 (MQ=255)                                                      gcgAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGCTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGa                                                                                        >  2:1071733/1‑133 (MQ=255)                                                       cgAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGAt                                                                                >  1:459771/1‑140 (MQ=255)                                                       cgAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGAt                                                                                >  2:1028845/1‑140 (MQ=255)                                                       cgAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGAt                                                                                >  1:710986/1‑140 (MQ=255)                                                         aaCAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTgg                                                                                  >  1:491763/1‑136 (MQ=255)                                                         aaCAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTgg                                                                                  <  2:491763/136‑1 (MQ=255)                                                              gTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGc                                                                         <  1:371287/140‑1 (MQ=255)                                                                 tGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTgg                                                                      >  2:1070490/1‑140 (MQ=255)                                                                    cGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGgcg                                                                   <  2:342296/140‑1 (MQ=255)                                                                    cGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGgcg                                                                   <  1:969805/140‑1 (MQ=255)                                                                    cGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGgcg                                                                   <  1:938111/140‑1 (MQ=255)                                                                    cGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGgcg                                                                   <  1:678037/140‑1 (MQ=255)                                                                    cGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGgcg                                                                   <  2:913000/140‑1 (MQ=255)                                                                      gAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGca                                                                 <  1:795718/140‑1 (MQ=255)                                                                      gAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGca                                                                 <  1:996634/140‑1 (MQ=255)                                                                                    gtcTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCgg                                                   <  2:710986/140‑1 (MQ=255)                                                                                          aGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGtca                                             <  1:456517/140‑1 (MQ=255)                                                                                            cGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGtca                                             >  2:65248/1‑138 (MQ=255)                                                                                            cGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGtca                                             <  1:65248/138‑1 (MQ=255)                                                                                             gAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTTGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGtcatca                                          <  2:981193/140‑1 (MQ=255)                                                                                                ccTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATcaaca                                       >  2:538059/1‑140 (MQ=255)                                                                                                 cTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCgg                                                   >  2:530026/1‑127 (MQ=255)                                                                                                 cTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCgg                                                   <  1:530026/127‑1 (MQ=255)                                                                                                 cTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATcaacaa                                      >  1:12872/1‑140 (MQ=255)                                                                                                  ttATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCaacaac                                     <  1:538059/140‑1 (MQ=255)                                                                                                   tATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAAcg                                    >  1:168461/1‑140 (MQ=255)                                                                                                     ttCCGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAAcgcg                                  >  1:373594/1‑140 (MQ=255)                                                                                                       ccGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTg                                <  2:83481/140‑1 (MQ=255)                                                                                                       ccGCGCAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTg                                <  2:263234/140‑1 (MQ=255)                                                                                                         gcgcAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGa                              <  1:215421/140‑1 (MQ=255)                                                                                                          cgcAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAg                             <  1:1028845/140‑1 (MQ=255)                                                                                                          cgcAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAg                             <  2:373594/140‑1 (MQ=255)                                                                                                          cgcAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAg                             >  2:202202/1‑140 (MQ=255)                                                                                                           gcAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTg                                <  2:641379/136‑1 (MQ=255)                                                                                                           gcAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTg                                >  1:641379/1‑136 (MQ=255)                                                                                                           gcAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAg                             >  2:657082/1‑139 (MQ=255)                                                                                                           gcAACTTTCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAg                             <  1:657082/139‑1 (MQ=255)                                                                                                                  tCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTTTGTATGCGTGGTGGGGTGCACAGTACGAAGGCGGTTGTCATCAACAACGCGTGGAGGCGATTAc                     <  2:82811/140‑1 (MQ=255)                                                                                                                  tCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTAc                     <  1:454666/140‑1 (MQ=255)                                                                                                                  tCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTAc                     <  1:202202/140‑1 (MQ=255)                                                                                                                  tCGCGGCAGCATAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTAc                     <  2:902177/140‑1 (MQ=255)                                                                                                                       gcagcaTAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCaa                <  2:565866/140‑1 (MQ=255)                                                                                                                         agcaTAACGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATa              >  1:407222/1‑140 (MQ=255)                                                                                                                               aCGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGagcag        <  2:168461/140‑1 (MQ=255)                                                                                                                                cGACACCAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCTTCATCAACAACGCGTGGAGGCGATTACGGCAATAGagcagc       >  1:48959/1‑140 (MQ=255)                                                                                                                                     ccAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGgaga  >  1:447826/1‑140 (MQ=255)                                                                                                                                     ccAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGgaga  >  1:668849/1‑140 (MQ=255)                                                                                                                                     ccAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGgaga  >  2:436336/1‑140 (MQ=255)                                                                                                                                     ccAATGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGgaga  >  1:202949/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        AGGTTGATGGCGGGATTTTGATTTGTGGTACTGGCGTCGGTATTTCGATAGCGGCGAACAAGTTTGCCGGAATTCGCGCGGTCGTCTGTAGCGAACCTTATTCCGCGCAACTTTCGCGGCAGCATAACGACACCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGA  >  NC_000913/4313522‑4313793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑