Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,575,592	C→T	pseudogene (1458/2721 nt)	yjiV →	putative uncharacterized protein YjiV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,575,592	0	C	T	100.0%	295.9 / NA	82	pseudogene (1458/2721 nt)	yjiV	putative uncharacterized protein YjiV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (48/34);  total (48/34)

CCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAACCCCTTATCTGCCTACCCATAT  >  NC_000913/4575459‑4575726                                                                                                                                      |                                                                                                                                       cccATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGc                                                                                                                                  <  2:583058/140‑1 (MQ=255)  ccATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCa                                                                                                                                 >  1:819438/1‑140 (MQ=255)    aTCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAAc                                                                                                                               <  1:204172/140‑1 (MQ=255)     tCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAAcc                                                                                                                              >  2:962633/1‑140 (MQ=255)      cGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCg                                                                                                                             >  2:970000/1‑140 (MQ=255)      cGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCg                                                                                                                             >  2:402302/1‑140 (MQ=255)      cGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCg                                                                                                                             >  1:92835/1‑140 (MQ=255)      cGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCg                                                                                                                             >  2:656050/1‑140 (MQ=255)       gTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGt                                                                                                                            >  2:401025/1‑140 (MQ=255)        tttAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGc                                                                                                                                  <  1:100227/133‑1 (MQ=255)        tttAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGc                                                                                                                                  >  2:100227/1‑133 (MQ=255)         ttAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGttt                                                                                                                          >  2:371699/1‑140 (MQ=255)           aGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGttttt                                                                                                                        >  1:646059/1‑140 (MQ=255)                   tGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCg                                                                                                                <  1:371699/140‑1 (MQ=255)                            tGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCat                                                                                                       <  2:36756/140‑1 (MQ=255)                            tGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCat                                                                                                       <  2:92835/140‑1 (MQ=255)                               gggCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATg                                                                                                    >  2:59399/1‑140 (MQ=255)                                   tGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACtt                                                                                                >  2:669793/1‑140 (MQ=255)                                      aTTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACttttt                                                                                             >  1:611300/1‑140 (MQ=255)                                               cGTCAATGCGGCAACTATTGATGTTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCa                                                                                    >  2:922982/1‑140 (MQ=255)                                                gTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCAc                                                                                   >  1:818270/1‑140 (MQ=255)                                                  cAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACgg                                                                                 <  1:59399/140‑1 (MQ=255)                                                          cAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTgg                                                                         >  1:998794/1‑140 (MQ=255)                                                                   atatTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCg                                                                <  2:646059/140‑1 (MQ=255)                                                                      ttatCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTa                                                             <  1:962633/140‑1 (MQ=255)                                                                       tatCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTaa                                                            >  1:523119/1‑140 (MQ=255)                                                                       tatCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTAATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTaa                                                            >  2:457630/1‑140 (MQ=255)                                                                        atCAATACATGGCTGAAAGAAGCATGGGCCTAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAAc                                                           >  1:370860/1‑140 (MQ=255)                                                                        atCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAAc                                                           >  1:718466/1‑140 (MQ=255)                                                                        atCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAAc                                                           >  2:541599/1‑140 (MQ=255)                                                                        atCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAAc                                                           >  1:39107/1‑140 (MQ=255)                                                                        atCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAAc                                                           >  1:35575/1‑140 (MQ=255)                                                                             tACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATa                                                      >  2:48255/1‑140 (MQ=255)                                                                             tACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTAATTTACCGAAAGAGCATATGACTTTATCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATa                                                      <  1:457630/140‑1 (MQ=255)                                                                               cATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATaaa                                                    <  1:392061/140‑1 (MQ=255)                                                                                aTGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAAc                                                           >  1:678433/1‑132 (MQ=255)                                                                                aTGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAAc                                                           <  2:678433/132‑1 (MQ=255)                                                                                  ggCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCaa                                                        <  1:513178/133‑1 (MQ=255)                                                                                  ggCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCaa                                                        >  2:513178/1‑133 (MQ=255)                                                                                  ggCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATc                                                 >  2:172000/1‑140 (MQ=255)                                                                                  ggCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATc                                                 >  2:758541/1‑140 (MQ=255)                                                                                  ggCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATc                                                 >  1:334279/1‑140 (MQ=255)                                                                                  ggCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATc                                                 >  1:210841/1‑140 (MQ=255)                                                                                  ggCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATc                                                 <  2:334279/140‑1 (MQ=255)                                                                                  ggCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATc                                                 >  1:846114/1‑140 (MQ=255)                                                                                  ggCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATc                                                 >  1:821551/1‑140 (MQ=255)                                                                                  ggCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATc                                                 >  2:549479/1‑140 (MQ=255)                                                                                       aaaGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGa                                            >  1:31622/1‑140 (MQ=255)                                                                                       aaaGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGa                                            >  2:565127/1‑140 (MQ=255)                                                                                        aagaagCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGAt                                           <  2:31622/140‑1 (MQ=255)                                                                                         agaagCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATa                                          <  2:998794/140‑1 (MQ=255)                                                                                           aagCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACg                                        <  2:523119/140‑1 (MQ=255)                                                                                            agCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACgg                                       <  2:818270/140‑1 (MQ=255)                                                                                                  ggCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTa                                 <  2:35575/140‑1 (MQ=255)                                                                                                  ggCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTa                                 <  1:401025/140‑1 (MQ=255)                                                                                                   gCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTaa                                >  2:671605/1‑140 (MQ=255)                                                                                                   gCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTaa                                >  2:125455/1‑140 (MQ=255)                                                                                                   gCCCAACTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTaa                                <  2:39107/140‑1 (MQ=255)                                                                                                         cTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCtt                          <  1:541599/140‑1 (MQ=255)                                                                                                         cTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCtt                          <  1:549479/140‑1 (MQ=255)                                                                                                         cTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCtt                          <  1:565127/140‑1 (MQ=255)                                                                                                         cTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCtt                          <  1:671605/140‑1 (MQ=255)                                                                                                         cTTACCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCtt                          <  1:402302/140‑1 (MQ=255)                                                                                                            aCCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCtt                          >  2:627019/1‑137 (MQ=255)                                                                                                            aCCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCtt                          <  1:627019/137‑1 (MQ=255)                                                                                                            aCCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAAc                       >  2:387154/1‑140 (MQ=255)                                                                                                            aCCGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAAGCTTaaa                       >  2:1074814/1‑139 (MQ=255)                                                                                                             ccGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAAcc                      <  1:970000/140‑1 (MQ=255)                                                                                                             ccGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAAcc                      <  2:210841/140‑1 (MQ=255)                                                                                                              cGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCtt                          <  1:656529/135‑1 (MQ=255)                                                                                                              cGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCtt                          >  2:656529/1‑135 (MQ=255)                                                                                                              cGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTa                         >  2:492870/1‑136 (MQ=255)                                                                                                              cGGACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTa                         <  1:492870/136‑1 (MQ=255)                                                                                                                gACCGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAACCCCt                   <  1:758541/140‑1 (MQ=255)                                                                                                                   cGCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAACCCCTTAt                >  1:481592/1‑140 (MQ=255)                                                                                                                    gCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAACCCCTTATc               >  2:987883/1‑140 (MQ=255)                                                                                                                    gCTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAACCCCTTATc               >  1:573615/1‑140 (MQ=255)                                                                                                                     cTTGCAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAACCCCTTATCt              >  1:24034/1‑140 (MQ=255)                                                                                                                         cAGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAACCCCTTATCTGCCt          <  2:24034/140‑1 (MQ=255)                                                                                                                          aGTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAACCCCTTATCTGCCTa         <  1:125455/140‑1 (MQ=255)                                                                                                                           gTACTGAAACCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAACCCCTTATCTGCCTAc        >  2:147071/1‑140 (MQ=255)                                                                                                                                 aaaCCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAACCCCTTATCTGCCTACCCatat  >  1:477680/1‑140 (MQ=255)                                                                                                                                 aaaCCTGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAACAAAATCCTGGATACGGCTTTTAAAGGCTTAACCCCTTATCTGCCTACCCatat  >  2:939688/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       CCCATCGTTTAGCGAAGTTGCTGATGCTGGGGGCTGGATTCAAAACCGTCAATGCGGCAACTATTGATATTATCAATACATGGCTGAAAGAAGCATGGGCCCAACTTACCGGACCGCTTGCAGTACTGAAACCCGATGGCAACCGTTTTTATTTACCGAAAGAGCATATGACTTTTTCTTTAATCACGGATGCCTGGATTTGCCCGGTAACCAATAAAATCCTGGATACGGCTTTTAAAGGCTTAACCCCTTATCTGCCTACCCATAT  >  NC_000913/4575459‑4575726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑