Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,634,014	A→G	D73G (GAC→GGC)	ytjC →	putative phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,634,014	0	A	G	100.0%	141.7 / NA	39	D73G (GAC→GGC)	ytjC	putative phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/22);  total (17/22)

CTGACCGCCAAAGGTGAGCAACAGGCGATGCAGGTGGCAACCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGACATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCACCGTTGACGGGCGTATTC  >  NC_000913/4633878‑4634145                                                                                                                                         |                                                                                                                                    cTGACCGCCAAAGGTGAGCAACAGGCGATGCAGGTGGCAACCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGcat                                                                                                                                  <  2:181222/140‑1 (MQ=255)  tGACCGCCAAAGGTGAGCAACAGGCGATGCAGGTGGCAACCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGcatc                                                                                                                                 <  2:372621/140‑1 (MQ=255)      cGCCAAAGGTGAGCAACAGGCGATGCAGGTGGCAACCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCt                                                                                                                             >  1:187183/1‑140 (MQ=255)       gCCAAAGGTGAGCAACAGGCGATGCAGGTGGCAACCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGca                                                                                                                                   >  2:687967/1‑133 (MQ=255)                gAGCAACAGGCGATGCAGGTGGCAACCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCg                                                                                                                   >  2:446005/1‑140 (MQ=255)                   cAACAGGCGATGCAGGTGGCAACCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCt                                                                                                                >  1:685015/1‑140 (MQ=255)                     aCAGGCGATGCAGGTGGCAACCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGc                                                                                                              <  1:792957/140‑1 (MQ=255)                      cAGGCGATGCAGGTGGCAACCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCg                                                                                                             <  2:286988/140‑1 (MQ=255)                      cAGGCGATGCAGGTGGCAACCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCg                                                                                                             <  2:5882/140‑1 (MQ=255)                          cGATGCAGGTGGCAACCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGaa                                                                                                         <  1:528800/140‑1 (MQ=255)                          cGATGCAGGTGGCAACCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGaa                                                                                                         <  1:519587/140‑1 (MQ=255)                          cGATGCAGGTGGCAACCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGaa                                                                                                         <  1:506746/140‑1 (MQ=255)                          cGATGCAGGTGGCAACCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGaa                                                                                                         <  1:478568/140‑1 (MQ=255)                            aTGCAGGTGGCAACCCATGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAAtt                                                                                                       <  2:966946/140‑1 (MQ=255)                                   tGGCAACCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATg                                                                                                <  2:685015/140‑1 (MQ=255)                                   tGGCAACCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATg                                                                                                >  1:351487/1‑140 (MQ=255)                                       aaCCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGgtg                                                                                            >  1:133541/1‑140 (MQ=255)                                           cGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCt                                                                                        <  2:537033/140‑1 (MQ=255)                                           cGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCt                                                                                        <  2:351487/140‑1 (MQ=255)                                                     agCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGa                                                                              <  1:405932/140‑1 (MQ=255)                                                     agCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGa                                                                              <  1:807870/140‑1 (MQ=255)                                                       cTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACa                                                                            >  1:1042727/1‑140 (MQ=255)                                                               tACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGAtt                                                                    >  1:161556/1‑140 (MQ=255)                                                                 cGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATtct                                                                  >  1:841284/1‑140 (MQ=255)                                                                 cGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATtct                                                                  <  2:1042727/140‑1 (MQ=255)                                                                 cGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATtct                                                                  >  1:985479/1‑140 (MQ=255)                                                                        catcaGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCg                                                           <  2:187183/140‑1 (MQ=255)                                                                        catcaGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCg                                                           <  2:144632/140‑1 (MQ=255)                                                                                gCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATtctc                                                                 >  2:541396/1‑126 (MQ=255)                                                                                gCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATtctc                                                                 <  1:541396/126‑1 (MQ=255)                                                                                    tttAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCgaag                                                        >  2:415893/1‑131 (MQ=255)                                                                                    tttAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCgaag                                                        <  1:415893/131‑1 (MQ=255)                                                                                                ccGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGc                                   <  2:841284/140‑1 (MQ=255)                                                                                                ccGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGc                                   <  2:921941/140‑1 (MQ=255)                                                                                                             gAAATCATCGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCAGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATgg                      >  1:1051186/1‑140 (MQ=255)                                                                                                                atcatcGCCCAGGCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCAc                   >  1:223091/1‑140 (MQ=255)                                                                                                                            gCCTGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCACCGTTGACGGGCg       >  2:828287/1‑140 (MQ=255)                                                                                                                               tGCGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCACCGTTGACGGGCGTAt    >  1:6079/1‑140 (MQ=255)                                                                                                                                 cGGCTGTGGCATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCACCGTTGACGGGCGTATTc  >  1:706331/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    CTGACCGCCAAAGGTGAGCAACAGGCGATGCAGGTGGCAACCCGTGCCAAAGAGCTTGGCATTACGCATATCATCAGTAGCGATTTAGGACGCACCCGGCGTACGGCGGAAATCATCGCCCAGGCCTGCGGCTGTGACATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCACCGTTGACGGGCGTATTC  >  NC_000913/4633878‑4634145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑