Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	381,052	A→G	pseudogene (209/417 nt)	yaiX ←	putative acyltransferase, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	381,052	0	A	G	100.0%	183.2 / NA	50	pseudogene (209/417 nt)	yaiX	putative acyltransferase, N‑terminal fragment
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/26);  total (24/26)

CGCGCGGGCCAAGTTGCGTGTTCGGGGAAATAATTCGCCCAGGCAAAATAATAACTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTT  >  NC_000913/380915‑381186                                                                                                                                          |                                                                                                                                       cgcgcgGGCCAAGTTGCGTGTTCGGGGAAATAATTCGCCCAGGCAAAATAATAACTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCa                                                                                                                                      >  2:310550/1‑140 (MQ=255)          cAAGTTGCGTGTTCGGGGAAATAATTCGCCCAGGCAAAATAATAACTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGc                                                                                                                             >  1:267250/1‑140 (MQ=255)                      tCGGGGAAATAATTCGCCCAGGCAAAATAATAACTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGa                                                                                                                 <  1:818812/140‑1 (MQ=255)                      tCGGGGAAATAATTCGCCCAGGCAAAATAATAACTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGa                                                                                                                 <  1:625803/140‑1 (MQ=255)                      tCGGGGAAATAATTCGCCCAGGCAAAATAATAACTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGa                                                                                                                 <  1:930446/140‑1 (MQ=255)                       cGGGGAAATAATTCGCCCAGGCAAAATAATAACTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGAc                                                                                                                <  1:789517/140‑1 (MQ=255)                          ggAAATAATTCGCCCAGGCAAAATAATAACTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACgat                                                                                                             <  1:310550/140‑1 (MQ=255)                             aataatTCGCCCAGGCAAAATAATAACTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACgatgat                                                                                                          <  2:20015/140‑1 (MQ=255)                                aatTCGCCCAGGCAAAATAATAACTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTAc                                                                                                       <  2:231757/140‑1 (MQ=255)                                  ttCGCCCAGGCAAAATAATAACTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTg                                                                                                     <  1:113684/140‑1 (MQ=255)                                      cccAGGCAAAATAATAACTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGgtac                                                                                                 >  1:557759/1‑140 (MQ=255)                                         aGGCAAAATAATAACTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGgtacgta                                                                                              >  1:1073975/1‑140 (MQ=255)                                             aaaaTAATAACTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTtg                                                                                          <  2:704956/140‑1 (MQ=255)                                              aaaTAATAACTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTtgt                                                                                         <  1:113965/140‑1 (MQ=255)                                              aaaTAATAACTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTtgt                                                                                         <  2:469294/140‑1 (MQ=255)                                                  aataaCTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTgcgc                                                                                     <  1:819062/140‑1 (MQ=255)                                                     aaCTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTa                                                                                  >  1:762934/1‑140 (MQ=255)                                                           tACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATg                                                                            >  2:978165/1‑140 (MQ=255)                                                           tACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATg                                                                            >  2:95812/1‑140 (MQ=255)                                                            aCGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGc                                                                           <  1:1079372/140‑1 (MQ=255)                                                             cGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGcc                                                                          >  2:842058/1‑140 (MQ=255)                                                                  aGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGtt                                                                     <  1:95812/140‑1 (MQ=255)                                                                  aGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGtt                                                                     >  2:692574/1‑140 (MQ=255)                                                                    gCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTg                                                                   <  2:557759/140‑1 (MQ=255)                                                                                               tAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTc                                                      >  1:357917/1‑126 (MQ=255)                                                                                               tAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTc                                                      <  2:357917/126‑1 (MQ=255)                                                                                               tAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATaaaa                                            >  1:525093/1‑136 (MQ=255)                                                                                               tAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATaaaa                                            <  2:525093/136‑1 (MQ=255)                                                                                               tAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACAtt                                        >  1:997734/1‑140 (MQ=255)                                                                                               tAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACAtt                                        >  2:145209/1‑140 (MQ=255)                                                                                                aaTTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTg                                       <  2:628606/140‑1 (MQ=255)                                                                                                aaTTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTg                                       <  1:145209/140‑1 (MQ=255)                                                                                                 aTTTATCGTATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGc                                      >  2:417674/1‑140 (MQ=255)                                                                                                 aTTTATCGTATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGc                                      >  2:1027716/1‑140 (MQ=255)                                                                                                  tttATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCg                                     >  2:1021794/1‑140 (MQ=255)                                                                                                     aTCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGAc                                  >  2:145544/1‑140 (MQ=255)                                                                                                       cGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCGGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCa                                >  1:1032707/1‑140 (MQ=255)                                                                                                       cGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCa                                >  2:358989/1‑140 (MQ=255)                                                                                                           tCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCg                            <  1:842058/140‑1 (MQ=255)                                                                                                           tCCGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTAATGGTACGTACTTGTGCGCCTAAATATGCCTGGGTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCg                            >  2:1003939/1‑140 (MQ=255)                                                                                                             cGGTAGCGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGtt                          <  1:145544/140‑1 (MQ=255)                                                                                                                 aGCGATAATTCCCTCTGGAGTTCGAGCGGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCtt                      <  2:1032707/140‑1 (MQ=255)                                                                                                                   cGATAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTcc                    <  2:1073975/140‑1 (MQ=255)                                                                                                                     aTAATTCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGc                  >  2:592753/1‑140 (MQ=255)                                                                                                                          tCCCTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCaa             <  1:1003939/140‑1 (MQ=255)                                                                                                                           cccTCTGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAAt            <  2:762934/140‑1 (MQ=255)                                                                                                                             ctctGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATaa          >  2:430181/1‑140 (MQ=255)                                                                                                                                tGGAGTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCg       <  1:592753/140‑1 (MQ=255)                                                                                                                                    gTTCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCAtt   >  2:409659/1‑140 (MQ=255)                                                                                                                                     ttCGAGCAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCAttt  >  2:460036/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       CGCGCGGGCCAAGTTGCGTGTTCGGGGAAATAATTCGCCCAGGCAAAATAATAACTTGTACGCCAAGGCGTGAACGCTGCCCGATATAACAACCTAATTTATCGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTT  >  NC_000913/380915‑381186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑