Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	423,296	A→G	D260G (GAC→GGC)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	423,296	0	A	G	100.0%	292.9 / NA	79	D260G (GAC→GGC)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/40);  total (39/40)

GAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTC  >  NC_000913/423157‑423431                                                                                                                                            |                                                                                                                                        gAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGa                                                                                                                                         <  1:591582/140‑1 (MQ=255)       tATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTggcaggca                                                                                                                                   <  1:1026956/140‑1 (MQ=255)       tATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTggcaggca                                                                                                                                   >  1:84615/1‑140 (MQ=255)       tATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGTAGGCa                                                                                                                                   >  2:954968/1‑140 (MQ=255)         tCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATa                                                                                                                                 >  1:429947/1‑140 (MQ=255)           gCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAAt                                                                                                                               <  2:886120/140‑1 (MQ=255)              aTGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGt                                                                                                                            <  1:64380/140‑1 (MQ=255)              aTGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGt                                                                                                                            >  2:32219/1‑140 (MQ=255)                gTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGG‑TTGGCAGGCATAATCGTGGc                                                                                                                         >  2:716667/1‑140 (MQ=255)                 tCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTCGCGGTTCCCATGCCTGGTTTGGCAGGCATAATCGTGGc                                                                                                                         >  2:379666/1‑140 (MQ=255)                  cGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCa                                                                                                                        <  2:94315/140‑1 (MQ=255)                  cGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCa                                                                                                                        <  1:478164/140‑1 (MQ=255)                    aTCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACg                                                                                                                      >  2:455879/1‑140 (MQ=255)                    aTCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACg                                                                                                                      <  2:242856/140‑1 (MQ=255)                      ccGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACggg                                                                                                                    <  2:897697/140‑1 (MQ=255)                      ccGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACggg                                                                                                                    <  1:431920/140‑1 (MQ=255)                        gCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGggtg                                                                                                                  <  1:455879/140‑1 (MQ=255)                             ttGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCt                                                                                                             <  1:475568/140‑1 (MQ=255)                                   gTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTcac                                                                                                       <  2:84615/140‑1 (MQ=255)                                      aTGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAAc                                                                                                    >  2:636876/1‑140 (MQ=255)                                              ttGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATc                                                                                            >  1:339686/1‑140 (MQ=255)                                               tgctgcGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCg                                                                                           >  2:719775/1‑140 (MQ=255)                                                gctgcGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGc                                                                                          >  2:1037250/1‑140 (MQ=255)                                                       ttGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGgcg                                                                                   <  1:719775/140‑1 (MQ=255)                                                        tGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGgcgc                                                                                  <  2:429947/140‑1 (MQ=255)                                                        tGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGgcgc                                                                                  >  2:101540/1‑140 (MQ=255)                                                          cGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGa                                                                                <  2:339686/140‑1 (MQ=255)                                                            acacAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACt                                                                              <  1:544900/140‑1 (MQ=255)                                                              acaATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTgg                                                                            <  2:418200/140‑1 (MQ=255)                                                                aaTACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTa                                                                          >  2:548545/1‑140 (MQ=255)                                                                aaTACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTa                                                                          <  1:954968/140‑1 (MQ=255)                                                                aaTACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTa                                                                          <  2:897380/140‑1 (MQ=255)                                                                aaTACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTa                                                                          <  1:253327/140‑1 (MQ=255)                                                                     gcagcaGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGt                                                                     <  1:636876/140‑1 (MQ=255)                                                                       agcagcTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGttt                                                                   >  1:644788/1‑140 (MQ=255)                                                                        gcagcTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTa                                                                  <  1:661277/140‑1 (MQ=255)                                                                        gcagcTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTa                                                                  >  1:982249/1‑140 (MQ=255)                                                                        gcagcTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTa                                                                  >  1:28556/1‑140 (MQ=255)                                                                            cTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGAc                                                                               >  1:1053562/1‑123 (MQ=255)                                                                            cTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtga                                                              >  1:1010923/1‑140 (MQ=255)                                                                            cTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtga                                                              >  1:592772/1‑140 (MQ=255)                                                                            cTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGAATCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGAc                                                                               <  2:1053562/123‑1 (MQ=255)                                                                              gggAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtgatg                                                            >  1:746995/1‑140 (MQ=255)                                                                                   tGCGGCTGGTGCTGGACGGCGTGCTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGGGGTGCTTGTGACAACCCCGAATCGCCCTGGCGCGACTGGAACTCGTTTAGTGATGATGGc                                                       >  2:693810/1‑140 (MQ=255)                                                                                       gCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACgg                                                   <  1:115827/140‑1 (MQ=255)                                                                                       gCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACgg                                                   >  1:638128/1‑140 (MQ=255)                                                                                        cTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGG‑TTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGgcg                                                 <  1:716667/140‑1 (MQ=255)                                                                                               tGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGAc                                           <  1:139518/140‑1 (MQ=255)                                                                                                   cGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACtggc                                       <  2:592772/140‑1 (MQ=255)                                                                                                   cGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACtggc                                       <  2:982249/140‑1 (MQ=255)                                                                                                      cGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACtggcttg                                    >  1:282326/1‑140 (MQ=255)                                                                                                      cGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACtggcttg                                    >  2:707975/1‑140 (MQ=255)                                                                                                      cGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACtggcttg                                    >  2:380341/1‑140 (MQ=255)                                                                                                      cGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACtggcttg                                    >  2:393913/1‑140 (MQ=255)                                                                                                      cGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACtggcttg                                    <  2:644788/140‑1 (MQ=255)                                                                                                      cGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACtggcttg                                    >  1:670702/1‑140 (MQ=255)                                                                                                      cGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACtggc                                       <  2:811223/137‑1 (MQ=255)                                                                                                      cGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACtggc                                       >  1:811223/1‑137 (MQ=255)                                                                                                      cGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACGGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACtggcttg                                    >  1:803322/1‑140 (MQ=255)                                                                                                       gtgtTTAACCACAGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACtggcttgg                                   <  1:393913/140‑1 (MQ=255)                                                                                                                   aGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCtt                       >  1:1007505/1‑140 (MQ=255)                                                                                                                   aGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCtt                       >  2:940486/1‑140 (MQ=255)                                                                                                                   aGTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCtt                       >  2:907103/1‑140 (MQ=255)                                                                                                                    gTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATg                              >  1:526548/1‑132 (MQ=255)                                                                                                                    gTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATg                              <  2:526548/132‑1 (MQ=255)                                                                                                                    gTGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTg                      >  1:90703/1‑140 (MQ=255)                                                                                                                     tGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGc                     >  1:883716/1‑140 (MQ=255)                                                                                                                     tGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGc                     >  1:368471/1‑140 (MQ=255)                                                                                                                     tGGCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGc                     <  1:907103/140‑1 (MQ=255)                                                                                                                       gCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCg                   <  1:940486/140‑1 (MQ=255)                                                                                                                       gCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCg                   <  2:670702/140‑1 (MQ=255)                                                                                                                       gCGATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCg                   >  1:239026/1‑140 (MQ=255)                                                                                                                         gATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGaa                 <  2:1010923/140‑1 (MQ=255)                                                                                                                         gATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGaa                 <  1:1037250/140‑1 (MQ=255)                                                                                                                         gATTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACGGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGaa                 <  2:803322/140‑1 (MQ=255)                                                                                                                          aTTCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAg                <  1:380341/140‑1 (MQ=255)                                                                                                                           ttCCCATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGc               <  1:130008/140‑1 (MQ=255)                                                                                                                             cccATGCCTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTg             >  1:356796/1‑140 (MQ=255)                                                                                                                                   ccTGGTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTAt       <  2:1007505/140‑1 (MQ=255)                                                                                                                                       gTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCt              >  1:476808/1‑129 (MQ=255)                                                                                                                                       gTTTGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCt              <  2:476808/129‑1 (MQ=255)                                                                                                                                        tttGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGGCAGCTTGCCGAAGCTGGATTATCAGTc  >  2:274968/1‑140 (MQ=255)                                                                                                                                        tttGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTc  >  1:227708/1‑140 (MQ=255)                                                                                                                                        tttGGCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTc  >  1:797230/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                        GAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTC  >  NC_000913/423157‑423431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑