Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	45,901	A→G	Y32C (TAT→TGT)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	45,901	0	A	G	100.0%	144.2 / NA	41	Y32C (TAT→TGT)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/13);  total (28/13)

CGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGT  >  NC_000913/45764‑46023                                                                                                                                          |                                                                                                                           cGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTtgtg                                                                                                                          <  1:642998/140‑1 (MQ=255)  gTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTgtgt                                                                                                                         <  2:629310/140‑1 (MQ=255)    aaCGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTgtgt                                                                                                                         <  2:786797/138‑1 (MQ=255)    aaCGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTgtgt                                                                                                                         >  1:786797/1‑138 (MQ=255)        ccgcACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGt                                                                                                                   >  2:240771/1‑140 (MQ=255)                aCTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATtg                                                                                                           >  1:721378/1‑140 (MQ=255)                         gCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTGGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGgcgc                                                                                                  <  2:709852/140‑1 (MQ=255)                          cAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCt                                                                                                 >  1:1026744/1‑140 (MQ=255)                                aCAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCa                                                                                           >  1:955931/1‑140 (MQ=255)                                         aCCATGCAAGCGTCCGGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGcc                                                                                  >  1:814820/1‑140 (MQ=255)                                              gCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGgcgc                                                                             >  1:514719/1‑140 (MQ=255)                                              gCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGgcgc                                                                             >  2:907882/1‑140 (MQ=255)                                                aaCCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTg                                                                           <  1:907882/140‑1 (MQ=255)                                                  ccGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGaa                                                                         >  1:854118/1‑140 (MQ=255)                                                     tCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACt                                                                      >  2:497586/1‑140 (MQ=255)                                                     tCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACt                                                                      <  2:1026744/140‑1 (MQ=255)                                                                        tcAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGc                                                   >  2:813779/1‑140 (MQ=255)                                                                                 cctcTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGgc                                          >  1:173372/1‑140 (MQ=255)                                                                                 cctcTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGgc                                          >  2:884275/1‑140 (MQ=255)                                                                                  ctctATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGgcg                                         >  2:900394/1‑140 (MQ=255)                                                                                  ctctATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGgcg                                         >  2:802101/1‑140 (MQ=255)                                                                                       ttCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGa                                    >  1:795907/1‑140 (MQ=255)                                                                                            cgccgcATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGACACTGGACGCTGACTGGATTGGCTTGATGGGCGCGGGAACGCt                               >  2:106466/1‑140 (MQ=255)                                                                                            cgccgcATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCt                               >  1:869610/1‑140 (MQ=255)                                                                                            cgccgcATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCt                               >  1:358614/1‑140 (MQ=255)                                                                                            cgccgcATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCt                               >  1:927140/1‑140 (MQ=255)                                                                                            cgccgcATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCt                               >  1:324628/1‑140 (MQ=255)                                                                                            cgccgcATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCt                               >  2:133013/1‑140 (MQ=255)                                                                                            cgccgcATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCt                               >  2:6642/1‑140 (MQ=255)                                                                                              ccgcATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCg                             >  2:729198/1‑140 (MQ=255)                                                                                              ccgcATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCg                             <  1:133013/140‑1 (MQ=255)                                                                                               cgcATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGc                            <  1:813779/140‑1 (MQ=255)                                                                                                    tttGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGc                       <  1:497586/140‑1 (MQ=255)                                                                                                      tGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTg                     <  2:173372/140‑1 (MQ=255)                                                                                                      tGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTg                     <  1:729198/140‑1 (MQ=255)                                                                                                             gggAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTg              >  1:312070/1‑140 (MQ=255)                                                                                                              ggAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTgg             <  2:358614/140‑1 (MQ=255)                                                                                                               gAAGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGc            <  1:884275/140‑1 (MQ=255)                                                                                                                aaGCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGca           >  1:849501/1‑140 (MQ=255)                                                                                                                  gCGGTGGTCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGcaca         >  2:660402/1‑140 (MQ=255)                                                                                                                     gtggtCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCg      <  2:579146/140‑1 (MQ=255)                                                                                                                     gtggtCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCg      >  2:918704/1‑140 (MQ=255)                                                                                                                       ggtCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCg      >  1:816223/1‑138 (MQ=255)                                                                                                                       ggtCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCg      <  2:816223/138‑1 (MQ=255)                                                                                                                         tCCGTTTCTGGATGGTTGTGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGt  <  1:802101/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                           CGTAACGCCGCACTGACTCTCATTGCAAAAAACAGGAATAACCATGCAACCGTCCAGAAACTTTGACGATCTCAAATTCTCCTCTATTCACCGCCGCATTTTGCTGTGGGGAAGCGGTGGTCCGTTTCTGGATGGTTATGTACTGGTAATGATTGGCGTGGCGCTGGAGCAACTGACGCCGGCGCTGAAACTGGACGCTGACTGGATTGGCTTGCTGGGCGCGGGAACGCTCGCCGGGCTGTTCGTTGGCACATCGCTGT  >  NC_000913/45764‑46023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑