Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	580,775	A→G	intergenic (‑330/‑59)	ylcI ← / → nohD	DLP12 prophage; DUF3950 domain‑containing protein YlcI/DLP12 prophage; putative DNA‑packaging protein NohD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	580,775	0	A	G	100.0%	192.2 / NA	53	intergenic (‑330/‑59)	ylcI/nohD	DLP12 prophage; DUF3950 domain‑containing protein YlcI/DLP12 prophage; putative DNA‑packaging protein NohD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/27);  total (26/27)

GGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTACCATTCAGAACTGGCAGGAA  >  NC_000913/580649‑580905                                                                                                                               |                                                                                                                                   ggcgACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCttttt                                                                                                                       >  1:1017259/1‑140 (MQ=255) ggcgACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCttttt                                                                                                                       >  1:394746/1‑140 (MQ=255) ggcgACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCttttt                                                                                                                       >  1:940517/1‑140 (MQ=255)  gcgACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGc                                                                                                                            >  1:610456/1‑134 (MQ=255)  gcgACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGc                                                                                                                            <  2:610456/134‑1 (MQ=255)  gcgACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTg                                                                                                                      >  2:527230/1‑140 (MQ=255)        tCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCctct                                                                                                                <  2:432610/140‑1 (MQ=255)        tCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCctct                                                                                                                >  2:96519/1‑140 (MQ=255)         cgcgGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTg                                                                                                               >  2:15181/1‑140 (MQ=255)                    gCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCttt                                                                                                    >  1:317044/1‑140 (MQ=255)                          tATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGt                                                                                              >  1:937314/1‑140 (MQ=255)                          tATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGt                                                                                              >  1:441066/1‑140 (MQ=255)                                aaTTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGt                                                                                        >  2:185588/1‑140 (MQ=255)                                aaTTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGt                                                                                        <  1:647789/140‑1 (MQ=255)                                  ttttCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGATTTTGTcc                                                                                      >  1:40476/1‑140 (MQ=255)                                  tttaCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTcc                                                                                      >  2:1046330/1‑140 (MQ=255)                                    ttCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCttt                                                                                                    <  2:791910/124‑1 (MQ=255)                                    ttCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCttt                                                                                                    >  1:791910/1‑124 (MQ=255)                                    ttCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGt                                                                                    <  1:96519/140‑1 (MQ=255)                                     tCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTg                                                                                   >  1:1043820/1‑140 (MQ=255)                                                   tttCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGa                                                                     >  1:132659/1‑140 (MQ=255)                                                    ttCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGaa                                                                    <  1:120910/140‑1 (MQ=255)                                                              cttcGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACaaaa                                                          >  2:640987/1‑140 (MQ=255)                                                                  gtcgtAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAagca                                                      >  1:646499/1‑140 (MQ=255)                                                                      tAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAgctg                                                  >  2:99783/1‑140 (MQ=255)                                                                       aaCTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAgctgg                                                 <  2:308196/140‑1 (MQ=255)                                                                              tGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACAt                                          >  2:730580/1‑140 (MQ=255)                                                                              tGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACAt                                          >  2:278911/1‑140 (MQ=255)                                                                                   ttatttaAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCg                                     >  2:504419/1‑140 (MQ=255)                                                                                    tatttaAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCgg                                    >  2:1014053/1‑140 (MQ=255)                                                                                      tttaAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTg                                  <  1:185588/140‑1 (MQ=255)                                                                                        taAAATCCCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCg                                <  2:1017259/140‑1 (MQ=255)                                                                                        taAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCg                                <  2:925823/140‑1 (MQ=255)                                                                                        taAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCg                                <  2:1050348/140‑1 (MQ=255)                                                                                          aaaTACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAg                              <  1:1046330/140‑1 (MQ=255)                                                                                             tACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTAt                           <  1:1022416/140‑1 (MQ=255)                                                                                                 cccTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGt                       >  1:890985/1‑140 (MQ=255)                                                                                                  ccTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTa                      <  1:278911/140‑1 (MQ=255)                                                                                                  acTGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTa                      <  2:327114/139‑1 (MQ=255)                                                                                                    tGAAAAGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTAcc                    >  2:394764/1‑140 (MQ=255)                                                                                                      aaaaGAAAGGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTACCAt                  <  1:285120/140‑1 (MQ=255)                                                                                                         agaaagGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTACCATTCa               <  1:620040/140‑1 (MQ=255)                                                                                                          gaaagGAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTACCATTCAg              >  2:983357/1‑140 (MQ=255)                                                                                                              ggAAACGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTACCATTCAGAACt          >  1:472655/1‑140 (MQ=255)                                                                                                                aaaCGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTACCATTCAGAACTgg        <  1:730580/140‑1 (MQ=255)                                                                                                                 aaCGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTACCATTCAGAACTGGc       <  1:1070813/140‑1 (MQ=255)                                                                                                                 aaCGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTACCATTCAGAACTGGc       <  2:60607/140‑1 (MQ=255)                                                                                                                 aaCGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTACCATTCAGAACTGGc       <  1:527230/140‑1 (MQ=255)                                                                                                                 aaCGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTACCATTCAGAACTGGc       <  1:983357/140‑1 (MQ=255)                                                                                                                  aCGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTACCATTCAGAACTGGCa      >  1:136782/1‑140 (MQ=255)                                                                                                                  aCGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTACCATTCAGAACTGGCa      <  2:1017145/140‑1 (MQ=255)                                                                                                                  aCGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTACCATTCAGAACTGGCa      <  2:766385/140‑1 (MQ=255)                                                                                                                  aCGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTACCATTCAGAACTGGCa      <  1:1014053/140‑1 (MQ=255)                                                                                                                   cGACAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTACCATTCAGAACTGGCAg     <  2:890985/140‑1 (MQ=255)                                                                                                                      cAGGTGCTGGAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTACCATTCAGAACTGGCAGGaa  <  2:136782/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                   GGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCGTACCATTCAGAACTGGCAGGAA  >  NC_000913/580649‑580905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑