Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	590,002	A→G	V318A (GTT→GCT)	nfrA ←	bacteriophage N4 receptor, outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	590,002	0	A	G	100.0%	98.9 / NA	28	V318A (GTT→GCT)	nfrA	bacteriophage N4 receptor, outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/21);  total (7/21)

CAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGATAACAGATAGAGCCAACTTTTCTCTTGTGCGTCCGTGGTAAATAGTGGCTTATTTTCAA  >  NC_000913/589868‑590134                                                                                                                                       |                                                                                                                                      cAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGGGGCGGGCAGGTTTTTCGCTGCGTCGTTCTGGCCCTTTTTTTACAGCACCCGGGGGGCCGCGCCCACAGCATAc                                                                                                                                  >  2:498012/1‑140 (MQ=255)  aGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGGAGGTTTTTTGGTTCGTCGTACTGACCCTCTTTTTACAGCACCCGGAGCGGCGCGCCAAAAACATAca                                                                                                                                 >  2:791577/1‑139 (MQ=255)                     gagacagcAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTg                                                                                                              <  1:342867/133‑1 (MQ=255)                      cGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGt                                                                                                             <  1:791577/140‑1 (MQ=255)                       gCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTa                                                                                                            <  2:898555/140‑1 (MQ=255)                                                         gCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAgc                                                                         <  1:498012/140‑1 (MQ=255)                                                          gggggAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAgcg                                                                        <  1:873662/139‑1 (MQ=255)                                                          cGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAgcg                                                                        <  1:434273/140‑1 (MQ=255)                                                          cGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAgcg                                                                        <  1:1006911/140‑1 (MQ=255)                                                          cGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAgcg                                                                        <  1:210773/140‑1 (MQ=255)                                                           ggggAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAgcgc                                                                       <  1:433790/140‑1 (MQ=255)                                                           ggggAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAgcgc                                                                       <  1:48147/140‑1 (MQ=255)                                                            gggAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAGCGCt                                                                      <  1:30305/140‑1 (MQ=255)                                                                 ggTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTTTCGGCCAAACGTACCACAT‑AAATTGCCAACGCCCCGACGGGGGTAGCGCTGTAtt                                                                 >  2:433790/1‑140 (MQ=255)                                                                  gTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAGCGCTGTAttt                                                                <  1:739152/140‑1 (MQ=255)                                                                   tGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGGAAACTGTACCGTAT‑AATTTGCCAACGCCTGGACGGGGGTAGCGGTGTAtttt                                                               >  2:173885/1‑139 (MQ=255)                                                                      cGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGGAAACTGTACCCGAT‑AATTTGCCAACGCCCGGACGGGGGTAGCGCTGTATTTTGac                                                            >  2:873662/1‑139 (MQ=255)                                                                      cGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGAt                                                            <  1:331614/140‑1 (MQ=255)                                                                                   cgcTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGATAACAGATAGAGcc                                               <  1:102342/140‑1 (MQ=255)                                                                                   agacaggtcgtACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGATAACAGATAGAGcc                                               <  1:265893/134‑1 (MQ=255)                                                                                       gCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGGAAAACGGACCCTAT‑AATACCCCCAAACCTGAAAGGGGGTAGCGCGGGATTTTAATAAAAGAAAGAGACAACt                                           >  2:102342/1‑140 (MQ=255)                                                                                                     gtcttTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGATAACAGATAGAGCCAACTTTTCTCTTGTGCGt                             <  1:622826/139‑1 (MQ=255)                                                                                                      tcttTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGATAACAGATAGAGCCAACTTTTCTCTTGTGCGTc                            <  1:1015778/140‑1 (MQ=255)                                                                                                       cttTTAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGATAACAGATAGAGCCAACTTTTCTCTTGTGCGTcc                           <  1:173885/140‑1 (MQ=255)                                                                                                        ttttAACAGCACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGATAACAGATAGAGCCAACTTTTCTCTTGTGCGTCCg                          <  1:393453/140‑1 (MQ=255)                                                                                                                 cACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTTGGCAAACTGTACCGTAT‑AATTTTCCAACGCCTGAACGGGGGTAGGGCCGTATTTAGATAAAAGATAGAGCCAACTTTTTTCTTGTGCGTCCGGGGTaaaaa                 >  2:589443/1‑138 (MQ=255)                                                                                                                 cACCGGTAGCGTCGCGCCAACAGCATACTGGCGGTTGTCGGGAAAACGTACCGGATAAATTGCCCAACCCCGGAAGGGGGTT‑GCGCCGTATTTTTATAAAAAAAAGAGCCAACATTTTTTTTTTTCGTCCGGGGGAAATa                 >  2:1015778/1‑140 (MQ=255)                                                                                                                           gTCGCGCCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGATAACAGATAGAGCCAACTTTTCTCTTGTGCGTCCGTGGTAAATAGTGGCTTAtt       <  1:50507/140‑1 (MQ=255)                                                                                                                             cgcgCCAACAGCATACTGGCGGTTTTCGGCAAACTTTACCGTAT‑AATTCGCCAACGCCTGAAAGGGGGTGGGGCTGTTTTTAGATAAAAAATAGAGCCAAATTTTTTTTTTTGGGGCCGGGGGAAATAGGGGCTtttttt     >  2:896540/1‑140 (MQ=255)                                                                                                                                gcCAACAGCATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGATAACAGATAGAGCCAACTTTTCTCTTGTGCGTCCGTGGTAAATAGTGGCTTATTTTCaa  <  2:646590/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      CAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAACTGTACCGTAT‑AATTCGCCAACGCCTGAACGGGGTTAGCGCTGTATTTAGATAACAGATAGAGCCAACTTTTCTCTTGTGCGTCCGTGGTAAATAGTGGCTTATTTTCAA  >  NC_000913/589868‑590134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑