Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	629,623	T→C	G108G (GGT→GGC)	entH →	proofreading thioesterase in enterobactin biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	629,623	0	T	C	100.0%	203.0 / NA	57	G108G (GGT→GGC)	entH	proofreading thioesterase in enterobactin biosynthesis
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/23);  total (34/23)

AACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAA  >  NC_000913/629488‑629754                                                                                                                                        |                                                                                                                                    aaCGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGc                                                                                                                                 <  1:877362/140‑1 (MQ=255)        ggATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCa                                                                                                                          >  2:775130/1‑140 (MQ=255)              aTGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTg                                                                                                                    >  2:197839/1‑140 (MQ=255)                              tgaCCCGCGACGGACAGTGTGTGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTc                                                                                                    <  2:815629/140‑1 (MQ=255)                                aCCCGCGACGGACAGTGTGTGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGa                                                                                                  >  1:870287/1‑140 (MQ=255)                                aCCCGCGACGGACAGTGTGTGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGa                                                                                                  >  1:588220/1‑140 (MQ=255)                                     cgACGGACAGTGTGTGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAAc                                                                                             >  1:904036/1‑140 (MQ=255)                                          gACAGTGTGTGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAgggg                                                                                        <  2:90681/140‑1 (MQ=255)                                             aGTGTGTGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGggcgg                                                                                     <  2:466379/140‑1 (MQ=255)                                               tgtgtgGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGgcggcg                                                                                   >  2:127248/1‑140 (MQ=255)                                               tgtgtgGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGgcggcg                                                                                   >  1:764267/1‑140 (MQ=255)                                                       aGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCa                                                                           <  2:870287/140‑1 (MQ=255)                                                         gCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGCAAGGCACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGACATCGTCGTTTTCGATGAACAGGCGCGGCGTTGCTGCACt                                                                         >  2:755822/1‑140 (MQ=255)                                                         gCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGt                                                                                  <  2:318007/131‑1 (MQ=255)                                                         gCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGt                                                                                  >  1:318007/1‑131 (MQ=255)                                                         gCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACt                                                                         >  1:446677/1‑140 (MQ=255)                                                         gCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACt                                                                         >  1:125437/1‑140 (MQ=255)                                                         gCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACt                                                                         >  2:411885/1‑140 (MQ=255)                                                         gCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACt                                                                         >  1:204074/1‑140 (MQ=255)                                                         gCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACt                                                                         >  1:923487/1‑140 (MQ=255)                                                         gCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACt                                                                         >  1:950973/1‑140 (MQ=255)                                                          cacaGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACtt                                                                        <  2:764267/140‑1 (MQ=255)                                                           acaGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTg                                                                       >  1:77826/1‑140 (MQ=255)                                                            caGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGt                                                                      <  2:446677/140‑1 (MQ=255)                                                                 cTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCt                                                                 >  2:913865/1‑140 (MQ=255)                                                                    aaTGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTggg                                                              <  2:588220/140‑1 (MQ=255)                                                                      tGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTa                                                            >  2:482027/1‑140 (MQ=255)                                                                         aaCACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACgg                                                         <  1:913865/140‑1 (MQ=255)                                                                         aaCACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACgg                                                         <  2:204074/140‑1 (MQ=255)                                                                         aaCACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACgg                                                         <  2:923487/140‑1 (MQ=255)                                                                               ccATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGttt                                                   <  2:904036/140‑1 (MQ=255)                                                                                cATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACgg                                                         >  1:21908/1‑133 (MQ=255)                                                                                cATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACgg                                                         <  2:21908/133‑1 (MQ=255)                                                                                cATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGtttt                                                  >  1:617819/1‑140 (MQ=255)                                                                                  tCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTgg                                                >  1:656477/1‑140 (MQ=255)                                                                                   cGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTggg                                               <  2:1033128/140‑1 (MQ=255)                                                                                             cTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGa                                     <  1:197839/140‑1 (MQ=255)                                                                                              tGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGAc                                    <  1:127248/140‑1 (MQ=255)                                                                                                aGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAg                                  >  1:730468/1‑140 (MQ=255)                                                                                                   gAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAAt                               <  2:721768/140‑1 (MQ=255)                                                                                                    aaaGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAAtt                              >  2:130641/1‑140 (MQ=255)                                                                                                    aaaGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAAtt                              >  1:619849/1‑140 (MQ=255)                                                                                                    aaaGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAAtt                              >  2:678760/1‑140 (MQ=255)                                                                                                      aGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAg                            >  1:2930/1‑140 (MQ=255)                                                                                                      aGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAg                            >  1:888699/1‑140 (MQ=255)                                                                                                        gTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGa                                     <  1:160078/129‑1 (MQ=255)                                                                                                        gTACGCGGCGTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGa                                     >  2:160078/1‑129 (MQ=255)                                                                                                            gcggcgTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATgaga                      >  2:261836/1‑140 (MQ=255)                                                                                                             cggcgTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGAt                     <  1:848742/140‑1 (MQ=255)                                                                                                             cggcgTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGAt                     >  2:226760/1‑140 (MQ=255)                                                                                                             cggcgTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGAt                     >  2:257867/1‑140 (MQ=255)                                                                                                                cgTCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGc                  >  1:127252/1‑140 (MQ=255)                                                                                                                  tCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAg                >  1:305951/1‑140 (MQ=255)                                                                                                                  tCTGCCAGCCGCTGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAg                >  1:274649/1‑140 (MQ=255)                                                                                                                              tGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGtt    <  2:656477/140‑1 (MQ=255)                                                                                                                              tGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGtt    <  1:695847/140‑1 (MQ=255)                                                                                                                              tGCATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGtt    <  1:257867/140‑1 (MQ=255)                                                                                                                                cATCTTGGCCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTaa  >  2:100852/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    AACGCTGGGATCGATGGCCGGATTTATGATGACCCGCGACGGACAGTGTGTGGTAGGCACAGAACTTAATGCAACACACCATCGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAA  >  NC_000913/629488‑629754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑