| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 668,204 | T→C | N5S (AAC→AGC) | mrdA ← | peptidoglycan DD‑transpeptidase MrdA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 668,204 | 0 | T | C | 98.3% | 209.7 / ‑4.6 | 60 | N5S (AAC→AGC) | mrdA | peptidoglycan DD‑transpeptidase MrdA |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (23/36); total (23/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
CGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGTTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATGGG > NC_000913/668073‑668333 | cGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGt > 1:7649/1‑140 (MQ=255) aaGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCAt > 2:750160/1‑140 (MQ=255) aaCAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCt < 2:537654/140‑1 (MQ=255) cAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGc < 2:465554/140‑1 (MQ=255) tGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGtttt > 1:378632/1‑140 (MQ=255) gATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTAc < 2:319855/140‑1 (MQ=255) ttATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTc > 1:248870/1‑140 (MQ=255) tataCAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCa < 1:939469/140‑1 (MQ=255) tataCAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCa < 1:574047/140‑1 (MQ=255) cAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGc < 1:691555/140‑1 (MQ=255) aGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCt < 2:200560/140‑1 (MQ=255) gTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTcc < 1:186832/140‑1 (MQ=255) gTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTcc < 2:378632/140‑1 (MQ=255) ttGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTccc > 2:547232/1‑140 (MQ=255) tGGCGATAAGCACGCCGGTCAGCAGCAAAATTCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGc > 2:147006/1‑135 (MQ=255) tGGCGATAAGCACGCCGGTCAGCAGCAAAATTCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGc < 1:147006/135‑1 (MQ=255) ggCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCtt > 2:502782/1‑140 (MQ=255) ggCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCtt > 2:467410/1‑140 (MQ=255) gCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTa > 2:930621/1‑140 (MQ=255) cGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCa < 2:36499/128‑1 (MQ=255) cGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCa > 1:36499/1‑128 (MQ=255) cGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTAt > 1:96542/1‑140 (MQ=255) cGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTAt > 1:1031287/1‑140 (MQ=255) cGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTAt > 2:461585/1‑140 (MQ=255) cGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTAt > 1:47181/1‑140 (MQ=255) aaGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTAtcact < 1:502782/140‑1 (MQ=255) gCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTAtcactca < 1:50212/140‑1 (MQ=255) cACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTAtcactcac < 2:995052/140‑1 (MQ=255) cACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTAtcactcac < 2:196767/140‑1 (MQ=255) aCGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACg < 1:785694/140‑1 (MQ=255) aCGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACg < 1:964411/140‑1 (MQ=255) gTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATa > 1:1068525/1‑140 (MQ=255) gTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATa < 2:1068525/140‑1 (MQ=255) tCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTAtcact > 1:376536/1‑129 (MQ=255) tCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTAtcact < 2:376536/129‑1 (MQ=255) agcaAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGAtg < 1:364432/140‑1 (MQ=255) gcaAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGAtgg < 1:750160/140‑1 (MQ=255) caAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGAtggt > 1:115588/1‑140 (MQ=255) caAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGAtggt < 2:1010103/140‑1 (MQ=255) aaTCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGAtggttgg < 2:734768/140‑1 (MQ=255) cAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGa < 2:47181/140‑1 (MQ=255) ggCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTcc < 2:115588/140‑1 (MQ=255) ggCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTcc < 2:112270/140‑1 (MQ=255) ccAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGcc < 2:764008/140‑1 (MQ=255) ccAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGcc < 2:137830/140‑1 (MQ=255) ccAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGcc < 2:1031287/140‑1 (MQ=255) cAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGTTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTAATAAGGATGGTTGGTGGTGATGCTCCACGccc < 2:289130/140‑1 (MQ=255) cAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGccc < 2:96542/140‑1 (MQ=255) cAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGccc < 2:453747/140‑1 (MQ=255) gcCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGCCCGGTa < 1:467410/140‑1 (MQ=255) gcgcACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGCCCGGTACAGAc < 1:912877/140‑1 (MQ=255) cgcACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGCCCGGTACAGCct > 2:179551/1‑140 (MQ=255) acaAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGcc < 1:402912/126‑1 (MQ=255) acaAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGcc > 2:402912/1‑126 (MQ=255) acaAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGCCCGGTACAGActctc > 1:273061/1‑140 (MQ=255) aaCAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGc > 1:593215/1‑140 (MQ=255) aGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGAc < 2:229782/140‑1 (MQ=255) cgcgGACTCTGCCGTATAGTCGCGAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCa < 1:619569/140‑1 (MQ=255) gcgAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATggg > 1:310414/1‑140 (MQ=255) gcgAAAAGAGCTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATggg > 1:941110/1‑140 (MQ=255) | CGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGTTCTGTAGTTTCATCCGCTGCGTTTTCTACTCAAAGCTCCCTTATCACTCACGGTGATAAGGATGGTTGGTGGTGATGCTCCACGCCCGGTACAGACTCTCTGCGACCAGCACGCGAACCAGCGGATGGG > NC_000913/668073‑668333 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |