Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	794,261	A→G	R128R (CGT→CGC)	modE ←	DNA‑binding transcriptional dual regulator ModE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	794,261	0	A	G	100.0%	197.4 / NA	54	R128R (CGT→CGC)	modE	DNA‑binding transcriptional dual regulator ModE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/24);  total (30/24)

TCATCCAGCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCG  >  NC_000913/794124‑794395                                                                                                                                          |                                                                                                                                       tCATCCAGCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCg                                                                                                                                      >  2:157843/1‑140 (MQ=255)   aTCCAGCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTTGTTGCggg                                                                                                                                    <  2:860064/140‑1 (MQ=255)   aTCCAGCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCggg                                                                                                                                    <  1:883290/140‑1 (MQ=255)             aGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTg                                                                                                                          <  1:316665/140‑1 (MQ=255)              gACGCGCGCCGCTTTGTGCGGTAATTCCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGTTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCCCTGGTTGCGGGCGTTGGTTTGc                                                                                                                         >  2:789205/1‑140 (MQ=255)                    cgccgcTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCa                                                                                                                        <  1:974606/135‑1 (MQ=255)                    cgccgcTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCa                                                                                                                        >  2:974606/1‑135 (MQ=255)                    cgccgcTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGaa                                                                                                                   <  1:764840/140‑1 (MQ=255)                    cgccgcTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGATGGTTTGCAGTGaa                                                                                                                   >  2:385209/1‑140 (MQ=255)                                cGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATc                                                                                                       <  2:648307/140‑1 (MQ=255)                                    aaTTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCgg                                                                                                   <  1:157843/140‑1 (MQ=255)                                           aCTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCcagcag                                                                                            >  2:50815/1‑140 (MQ=255)                                                      tgtTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGc                                                                                          <  2:782399/131‑1 (MQ=255)                                                      tgtTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGc                                                                                          >  1:782399/1‑131 (MQ=255)                                                         ttttCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCa                                                                              <  2:779619/140‑1 (MQ=255)                                                                  aGCCTGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCAt                                                                     <  2:992659/140‑1 (MQ=255)                                                                  aGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCAt                                                                     >  2:278031/1‑140 (MQ=255)                                                                  aGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCAt                                                                     <  1:278031/140‑1 (MQ=255)                                                                    ccAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCg                                                                   <  1:433288/140‑1 (MQ=255)                                                                            aCATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTaa                                                           <  2:136923/140‑1 (MQ=255)                                                                             cATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAac                                                          >  2:459919/1‑140 (MQ=255)                                                                              aTCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAaca                                                         >  1:545076/1‑140 (MQ=255)                                                                                  aCATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATc                                                     >  1:735516/1‑140 (MQ=255)                                                                                  aCATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGACGTCATCGTCACTTAACACATc                                                     >  1:762611/1‑140 (MQ=255)                                                                                    aTGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCaa                                                   <  1:59724/140‑1 (MQ=255)                                                                                      gCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAg                                                 >  2:671881/1‑140 (MQ=255)                                                                                          ttGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCtt                                             >  1:976778/1‑140 (MQ=255)                                                                                          ttGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCtt                                             >  2:122602/1‑140 (MQ=255)                                                                                           tGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCttt                                            >  1:881745/1‑140 (MQ=255)                                                                                             aaCGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCttttt                                          >  1:508563/1‑140 (MQ=255)                                                                                                gTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAaca                                                         >  2:1064702/1‑122 (MQ=255)                                                                                                gTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAaca                                                         <  1:1064702/122‑1 (MQ=255)                                                                                                  catcatGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTgg                                     >  1:918347/1‑140 (MQ=255)                                                                                                  catcatGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTgg                                     >  2:833493/1‑140 (MQ=255)                                                                                                    tcatGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGAt                                   >  1:1023979/1‑140 (MQ=255)                                                                                                    tcatGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGAt                                   >  2:742698/1‑140 (MQ=255)                                                                                                    tcatGATCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGAt                                   >  1:741858/1‑140 (MQ=255)                                                                                                         aTCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTgcg                              >  2:632551/1‑140 (MQ=255)                                                                                                          tCGCGGGCGGTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTgcgc                             <  1:661294/140‑1 (MQ=255)                                                                                                            gcgggcggTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCa                           <  1:671881/140‑1 (MQ=255)                                                                                                            gcgggcggTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCa                           >  2:725776/1‑140 (MQ=255)                                                                                                             cgggcggTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAg                          >  2:266039/1‑140 (MQ=255)                                                                                                              gggcggTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGt                         <  2:152879/140‑1 (MQ=255)                                                                                                               ggcggTGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTa                        <  2:762611/140‑1 (MQ=255)                                                                                                                    tGATGGTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCa                   >  2:244694/1‑140 (MQ=255)                                                                                                                        ggTACCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATaga               <  1:742698/140‑1 (MQ=255)                                                                                                                           aCCGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCt            <  2:976778/140‑1 (MQ=255)                                                                                                                            ccGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTg           >  1:210812/1‑140 (MQ=255)                                                                                                                             cGAACCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGa          >  2:105062/1‑140 (MQ=255)                                                                                                                               aaCCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAAt        <  1:789205/140‑1 (MQ=255)                                                                                                                                aCCACTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATc       <  1:632551/140‑1 (MQ=255)                                                                                                                                    cTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTc   >  1:6159/1‑140 (MQ=255)                                                                                                                                    cTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTc   >  1:106656/1‑140 (MQ=255)                                                                                                                                    cTGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTc   >  2:481885/1‑140 (MQ=255)                                                                                                                                     tGGTTGCGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCg  <  1:50815/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TCATCCAGCCCCAGACGCGCGCCGCTTTGTGCGGTAATTGCGACTTTCAGGCGTGTTTTTCCGTCAGCCAGTAAGACATCAACATGCTGTTGAACGTCATCATGATCGCGGGCGGTGATGGTACCGAACCACTGGTTACGGGCGCTGGTTTGCAGTGAAAAACGTGAGATCGCGGCCAGCAGGCTGTTCAGCGGCAGGGCGTCATCGTCACTTAACACATCAAAGGCTTTTTGCTGGATTTGCGCCAGTAAGTCATAGAGCTGAATCAGTCG  >  NC_000913/794124‑794395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑