Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	88,245	A→G	N73S (AAC→AGC)	cra →	DNA‑binding transcriptional dual regulator Cra

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	88,245	0	A	G	100.0%	297.0 / NA	81	N73S (AAC→AGC)	cra	DNA‑binding transcriptional dual regulator Cra
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/40);  total (41/40)

ATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTC  >  NC_000913/88108‑88376                                                                                                                                          |                                                                                                                                    aTACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAgca                                                                                                                                   <  1:526162/140‑1 (MQ=255)       tgtgAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCt                                                                                                                             >  1:1020225/1‑140 (MQ=255)       tgtgAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCt                                                                                                                             >  1:273907/1‑140 (MQ=255)       tgtgAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCt                                                                                                                             >  2:849882/1‑140 (MQ=255)              gACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCg                                                                                                                      <  1:395893/140‑1 (MQ=255)                cAAAACCGTTGAAAAAGTCATGGCTGTGGTTCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCa                                                                                                                    <  2:662104/140‑1 (MQ=255)                cAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCa                                                                                                                    <  2:925100/140‑1 (MQ=255)                      cGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTa                                                                                                              <  1:8093/140‑1 (MQ=255)                         tGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACt                                                                                                           <  2:147647/140‑1 (MQ=255)                           aaaaaGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTAt                                                                                                         >  1:708684/1‑140 (MQ=255)                               aGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTg                                                                                                     >  1:690143/1‑140 (MQ=255)                                gTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGa                                                                                                    >  1:904824/1‑140 (MQ=255)                                   aTGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACg                                                                                                 <  2:690143/140‑1 (MQ=255)                                              gCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGc                                                                                      <  2:904824/140‑1 (MQ=255)                                                gTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCaa                                                                                    <  1:1043727/140‑1 (MQ=255)                                                 tGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAAc                                                                                   >  1:343286/1‑140 (MQ=255)                                                 tGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAAc                                                                                   >  2:758864/1‑140 (MQ=255)                                                 tGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAAc                                                                                   >  2:329538/1‑140 (MQ=255)                                                  gAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACg                                                                                  <  2:190752/140‑1 (MQ=255)                                                  gAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACg                                                                                  <  2:494783/140‑1 (MQ=255)                                                      acaATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGt                                                                              >  2:574134/1‑140 (MQ=255)                                                          ttACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATc                                                                          <  1:170892/140‑1 (MQ=255)                                                             ccaccCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAAc                                                                       <  2:343286/140‑1 (MQ=255)                                                                cccGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAActgc                                                                    >  1:200432/1‑140 (MQ=255)                                                                 ccGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAActgct                                                                   >  1:161372/1‑140 (MQ=255)                                                                 ccGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAActgct                                                                   >  1:578231/1‑140 (MQ=255)                                                                  cGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAActgctg                                                                  >  1:210578/1‑140 (MQ=255)                                                                  cGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAActgctg                                                                  <  2:57357/140‑1 (MQ=255)                                                                  cGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAActgctg                                                                  >  1:57357/1‑140 (MQ=255)                                                                    aaCGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGAt                                                                >  1:982103/1‑140 (MQ=255)                                                                        ccGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGcc                                                            >  2:618922/1‑140 (MQ=255)                                                                        ccGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGcc                                                            >  1:257195/1‑140 (MQ=255)                                                                          gTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTg                                                          <  2:982103/140‑1 (MQ=255)                                                                           tGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGc                                                         >  2:56542/1‑140 (MQ=255)                                                                           tGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGc                                                         >  2:597283/1‑140 (MQ=255)                                                                             gCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTc                                                       <  1:280858/140‑1 (MQ=255)                                                                                 cTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCagaa                                                   >  2:255262/1‑140 (MQ=255)                                                                                  tGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCagaag                                                  >  2:998678/1‑140 (MQ=255)                                                                                       ttCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAg                                             <  1:396405/140‑1 (MQ=255)                                                                                       ttCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAg                                             <  2:468905/140‑1 (MQ=255)                                                                                        tCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGc                                            <  1:758864/140‑1 (MQ=255)                                                                                        tCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGc                                            <  2:200432/140‑1 (MQ=255)                                                                                           tGCTGGGCGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAg                                         >  2:211690/1‑140 (MQ=255)                                                                                           tGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGAACTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAg                                         >  1:356296/1‑140 (MQ=255)                                                                                              tGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACa                                      >  2:775814/1‑140 (MQ=255)                                                                                              tGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACa                                      <  2:578231/140‑1 (MQ=255)                                                                                              tGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACa                                      <  2:161372/140‑1 (MQ=255)                                                                                                   gCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGaa                                 <  1:849882/140‑1 (MQ=255)                                                                                                   gCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGaa                                 <  1:998678/140‑1 (MQ=255)                                                                                                     acacGTTCTATTGGTCTTGTGATCCCCGAACTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAAt                               <  2:356296/140‑1 (MQ=255)                                                                                                        cGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCg                            <  1:815354/140‑1 (MQ=255)                                                                                                        cGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCg                            <  2:1056057/140‑1 (MQ=255)                                                                                                             tATTGGTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATACGGTGCa                       <  1:329538/140‑1 (MQ=255)                                                                                                                 ggTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                   <  2:1020225/140‑1 (MQ=255)                                                                                                                 ggTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                   <  2:617214/140‑1 (MQ=255)                                                                                                                 ggTCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                   >  2:454610/1‑140 (MQ=255)                                                                                                                   tCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                   <  2:481530/138‑1 (MQ=255)                                                                                                                   tCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGa                   >  1:481530/1‑138 (MQ=255)                                                                                                                   tCTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGc                 <  1:364144/140‑1 (MQ=255)                                                                                                                    cTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCa                >  1:487791/1‑140 (MQ=255)                                                                                                                    cTTGTGATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCa                >  1:340077/1‑140 (MQ=255)                                                                                                                       gtgATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGc                 >  1:303805/1‑136 (MQ=255)                                                                                                                       gtgATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGc                 <  2:303805/136‑1 (MQ=255)                                                                                                                       gtgATCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCt             >  2:204530/1‑140 (MQ=255)                                                                                                                         gATCCCCGATCTGGAGAGCACCAGCTATACCCGCATAGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCttt           >  2:57678/1‑140 (MQ=255)                                                                                                                          aTCCCCGATCTGGAGAGCACCAGCTATACCCGCATAGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCtttt          <  1:57678/140‑1 (MQ=255)                                                                                                                           tCCCCGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTa         <  1:211690/140‑1 (MQ=255)                                                                                                                            ccccGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTa         >  1:944472/1‑139 (MQ=255)                                                                                                                            ccccGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTa         <  2:944472/139‑1 (MQ=255)                                                                                                                             cccGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACa       >  2:786219/1‑140 (MQ=255)                                                                                                                             cccGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACa       >  1:263675/1‑140 (MQ=255)                                                                                                                             cccGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACa       >  1:136736/1‑140 (MQ=255)                                                                                                                             cccGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACa       <  1:56542/140‑1 (MQ=255)                                                                                                                             cccGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACa       >  1:590719/1‑140 (MQ=255)                                                                                                                             cccGATCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACa       <  2:487791/140‑1 (MQ=255)                                                                                                                                 aTCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACa       >  2:637410/1‑136 (MQ=255)                                                                                                                                 aTCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACa       <  1:637410/136‑1 (MQ=255)                                                                                                                                 aTCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGt   >  2:799109/1‑140 (MQ=255)                                                                                                                                 aTCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGt   >  2:924366/1‑140 (MQ=255)                                                                                                                                  tCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGc                 <  2:949132/125‑1 (MQ=255)                                                                                                                                  tCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGc                 >  1:949132/1‑125 (MQ=255)                                                                                                                                  tCTGGAGAGCACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTc  <  2:210578/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    ATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCTAACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTC  >  NC_000913/88108‑88376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑