Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	902,160	A→G	F31L (TTC→CTC)	artQ ←	L‑arginine ABC transporter membrane subunit ArtQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	902,160	0	A	G	100.0%	132.7 / NA	37	F31L (TTC→CTC)	artQ	L‑arginine ABC transporter membrane subunit ArtQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/15);  total (22/15)

TCAGCAGCAGCTGCGAGGAGCCAAAATAGATAAACAGCACCACCAGAATTTCTGGCAGGCCACGCAGAATGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAACATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAA  >  NC_000913/902025‑902293                                                                                                                                        |                                                                                                                                      tCAGCAGCAGCTGCGAGGAGCCAAAATAGATAAACAGCACCACCAGAATTTCTGGCAGGCCACGCAGAATGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATc                                                                                                                                   >  2:375718/1‑140 (MQ=255)          gcTGCGAGGAGCCAAAATAGATAAACAGCACCACCAGAATTTCTGGCAGGCCACGCAGAATGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATcgccagcgcc                                                                                                                          >  1:529481/1‑140 (MQ=255)              cGAGGAGCCAAAATAGATAAACAGCACCACCAGAATTTCTGGCAGGCCACGCAGAATGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGcc                                                                                                                      <  2:670089/140‑1 (MQ=255)                     ccAAAATAGATAAACAGCACCACCAGAATTTCTGGCAGGCCACGCAGAATGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGAcaat                                                                                                               <  2:758822/140‑1 (MQ=255)                     ccAAAATAGATAAACAGCACCACCAGAATTTCTGGCAGGCCACGCAGAATGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGAcaat                                                                                                               >  2:182282/1‑140 (MQ=255)                         aaTAGATAAACAGCACCACCAGAATTTCTGGCAGGCCACGCAGAATGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGAcaat                                                                                                               <  2:666526/136‑1 (MQ=255)                         aaTAGATAAACAGCACCACCAGAATTTCTGGCAGGCCACGCAGAATGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGAcaat                                                                                                               >  1:666526/1‑136 (MQ=255)                          aTAGATAAACAGCACCACCAGAATTTCTGGCAGGCCACGCAGAATGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATg                                                                                                          <  2:641929/140‑1 (MQ=255)                                      caccacCAGAATTTCTGGCAGGCCACGCAGAATGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATg                                                                                                          <  1:151864/128‑1 (MQ=255)                                      caccacCAGAATTTCTGGCAGGCCACGCAGAATGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATg                                                                                                          >  2:151864/1‑128 (MQ=255)                                      caccacCAGAATTTCTGGCAGGCCACGCAGAATGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCaa                                                                                              >  2:103381/1‑140 (MQ=255)                                        ccaccaGAATTTCTGGCAGGCCACGCAGAATGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAgg                                                                                            >  2:872481/1‑140 (MQ=255)                                        ccaccaGAATTTCTGGCAGGCCACGCAGAATGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAgg                                                                                            >  1:5614/1‑140 (MQ=255)                                                             cACGCAGAATGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGgcggcg                                                                       <  2:184739/140‑1 (MQ=255)                                                                     aTGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTaa                                                               >  1:965429/1‑140 (MQ=255)                                                                     aTGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTaa                                                               >  1:409997/1‑140 (MQ=255)                                                                              aGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGaaaaa                                                       >  1:639785/1‑139 (MQ=255)                                                                              aGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGaaaaa                                                       <  2:639785/139‑1 (MQ=255)                                                                                cgcTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAatt                                                    >  1:574417/1‑140 (MQ=255)                                                                                cgcTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAatt                                                    >  2:696473/1‑140 (MQ=255)                                                                                  cTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAattca                                                  <  2:409997/140‑1 (MQ=255)                                                                                     aaCCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAattcattc                                               >  1:1025922/1‑140 (MQ=255)                                                                                              aCGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAAt                                      <  1:300162/140‑1 (MQ=255)                                                                                                       gACGCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTgg                             >  1:201055/1‑140 (MQ=255)                                                                                                          gCCATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAAc                          >  1:560184/1‑140 (MQ=255)                                                                                                            cATTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCa                        >  2:617831/1‑140 (MQ=255)                                                                                                             aTTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGTCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCAt                       <  1:862538/140‑1 (MQ=255)                                                                                                             aTTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCAt                       <  2:785403/140‑1 (MQ=255)                                                                                                             aTTTTGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCAt                       <  2:499279/140‑1 (MQ=255)                                                                                                                 tGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAAGGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCAt                       >  2:745489/1‑136 (MQ=255)                                                                                                                 tGCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCAt                       <  1:745489/136‑1 (MQ=255)                                                                                                                  gCCGACTCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATttgtt                  <  2:1025922/140‑1 (MQ=255)                                                                                                                        tCCCATACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGAt            >  2:918611/1‑140 (MQ=255)                                                                                                                            aTACCGCAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGtt        >  2:761562/1‑140 (MQ=255)                                                                                                                                  cAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTaa  >  2:950157/1‑140 (MQ=255)                                                                                                                                  cAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTaa  <  2:560184/140‑1 (MQ=255)                                                                                                                                  cAAAGAGCATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTaa  >  1:114799/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      TCAGCAGCAGCTGCGAGGAGCCAAAATAGATAAACAGCACCACCAGAATTTCTGGCAGGCCACGCAGAATGGTTACCAGCGCTGAACCTGCCCACGCGACAGGACGCCATTTTGCCGACTCCCATACCGCAAAGAACATCGCCAGCGCCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAA  >  NC_000913/902025‑902293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑