Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 986,775 C→T E70K (GAA→AAA)  ompF ← outer membrane porin F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913986,7750CT100.0% 152.2 / NA 43E70K (GAA→AAA) ompFouter membrane porin F
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/23);  total (20/23)

CGTATTTAAGACCCGCGAATGCCAGACGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGA  >  NC_000913/986646‑986909
                                                                                                                                 |                                                                                                                                      
cGTATTTAAGACCCGCGAATGCCAGACGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTaaaa                                                                                                                              <  1:327584/140‑1 (MQ=255)
  tATTTAAGACCCGCGAATGCCAGACGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAAcc                                                                                                                            <  2:859723/140‑1 (MQ=255)
      tAAGACCCGCGAATGCCAGACGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGa                                                                                                                        <  1:926217/140‑1 (MQ=255)
               cgAATGCCAGACGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAg                                                                                                               >  1:705364/1‑140 (MQ=255)
               cgAATGCCAGACGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAg                                                                                                               >  1:406004/1‑140 (MQ=255)
                 aaTGCCAGACGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGt                                                                                                             <  2:375539/140‑1 (MQ=255)
                       aGACGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTc                                                                                                       >  1:785436/1‑140 (MQ=255)
                         aCGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGc                                                                                                     <  2:336721/140‑1 (MQ=255)
                            cgTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCAt                                                                                                  >  1:1004299/1‑140 (MQ=255)
                               tttGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGc                                                                                               <  2:785436/140‑1 (MQ=255)
                                 tGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCa                                                                                             <  1:820505/140‑1 (MQ=255)
                                        aGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAAc                                                                                      <  2:623804/140‑1 (MQ=255)
                                              aGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGtttt                                                                                <  2:705364/140‑1 (MQ=255)
                                                    aGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGt                                                                          >  1:358786/1‑140 (MQ=255)
                                                    aGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGt                                                                          >  2:631126/1‑140 (MQ=255)
                                                             agagTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTgg                                                                 <  2:508289/140‑1 (MQ=255)
                                                               agTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGaa                                                               <  1:948116/140‑1 (MQ=255)
                                                                    ttACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAaata                                                          >  1:1003482/1‑140 (MQ=255)
                                                                    ttACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAaata                                                          >  1:1004762/1‑140 (MQ=255)
                                                                    ttACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAaata                                                          >  1:746265/1‑140 (MQ=255)
                                                                                 tataTTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACa                                             >  1:849318/1‑140 (MQ=255)
                                                                                  atatTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAg                                            <  1:551506/140‑1 (MQ=255)
                                                                                    atTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCt                                          <  2:1004762/140‑1 (MQ=255)
                                                                                    atTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCt                                          <  2:260536/140‑1 (MQ=255)
                                                                                    atTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCt                                          <  1:631126/140‑1 (MQ=255)
                                                                                       cccACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTa                                       <  2:746265/140‑1 (MQ=255)
                                                                                          aCTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGATGGGCGTAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTa                                       >  2:150493/1‑137 (MQ=255)
                                                                                          aCTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGATGGGCGTAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTa                                       <  1:150493/137‑1 (MQ=255)
                                                                                          aCTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCg                                    >  2:207056/1‑140 (MQ=255)
                                                                                          aCTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCg                                    <  1:566198/140‑1 (MQ=255)
                                                                                          aCTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCg                                    >  2:518270/1‑140 (MQ=255)
                                                                                             gACCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCAATGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTAc                                 <  1:943615/140‑1 (MQ=255)
                                                                                              aCCATAACCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACa                                <  1:207056/140‑1 (MQ=255)
                                                                                                   aaCCGGTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCt                           >  1:26663/1‑140 (MQ=255)
                                                                                                        gTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAg                               >  1:367507/1‑131 (MQ=255)
                                                                                                        gTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAg                               <  2:367507/131‑1 (MQ=255)
                                                                                                        gTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACttt                      >  2:44967/1‑140 (MQ=255)
                                                                                                        gTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACttt                      >  2:1030400/1‑140 (MQ=255)
                                                                                                        gTCAGATCGGAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACttt                      >  1:740351/1‑140 (MQ=255)
                                                                                                                ggAATTGATTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCAt              <  2:849318/140‑1 (MQ=255)
                                                                                                                       aTTTGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGtt       >  1:641504/1‑140 (MQ=255)
                                                                                                                          tGAGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTtata    <  1:518270/140‑1 (MQ=255)
                                                                                                                            aGTTTTCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGa  >  1:263433/1‑140 (MQ=255)
                                                                                                                                 |                                                                                                                                      
CGTATTTAAGACCCGCGAATGCCAGACGCGTTTTGTTACCAGTTTGAGCGTCAGCGCCTTCAGAGTTGTTACCCTGGAAGTTATATTCCCACTGACCATAACCGGTCAGATCGGAATTGATTTGAGTTTCCCCTTTAAAACCAAGACGGGCATAGGTCATGTCGCCATTGCCACCGTAACTGTTTTCACCGTTACCCTTGGAAAAATAATGCAGACCAACAGCTTTACCGTACAGATCTACTTTGTTGCCATCTTTGTTATAGA  >  NC_000913/986646‑986909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑