Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,141,297	C→T	R1024H (CGT→CAT)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,141,297	0	C	T	100.0%	183.6 / NA	52	R1024H (CGT→CAT)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/24);  total (28/24)

TTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGACGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAGGTTCGACCGCAGCTACCGTTTCAACTTCTGCTGCGACTTCAGCGACTA  >  NC_000913/1141161‑1141426                                                                                                                                         |                                                                                                                                  ttGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGAtgcg                                                                                                                                >  1:1023091/1‑140 (MQ=255) ttGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGAtgcg                                                                                                                                >  1:529247/1‑140 (MQ=255) ttGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGAtgcg                                                                                                                                <  2:605201/140‑1 (MQ=255)  tGATTACTTTGAGCTAATTATTACTCAACAGGTCGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCgg                                                                                                                               >  1:1063215/1‑140 (MQ=255)    aTTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTg                                                                                                                             >  1:161136/1‑140 (MQ=255)     ttACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGc                                                                                                                            <  1:405076/140‑1 (MQ=255)             aGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTcc                                                                                                                        <  1:397107/136‑1 (MQ=255)             aGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTcc                                                                                                                        >  2:397107/1‑136 (MQ=255)               cTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACa                                                                                                                  >  1:1031568/1‑140 (MQ=255)                  attattACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACatat                                                                                                               <  2:161136/140‑1 (MQ=255)                    tattaCTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTc                                                                                                             <  1:1035339/140‑1 (MQ=255)                    tattaCTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTc                                                                                                             <  1:682529/140‑1 (MQ=255)                      ttaCTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCg                                                                                                           <  2:617390/140‑1 (MQ=255)                            aaCAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGaa                                                                                                                    <  1:2043/125‑1 (MQ=255)                            aaCAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGaa                                                                                                                    >  2:2043/1‑125 (MQ=255)                            aaCAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTc                                                                                                             >  2:755249/1‑132 (MQ=255)                            aaCAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTc                                                                                                             <  1:755249/132‑1 (MQ=255)                            aaCAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTg                                                                                                     >  2:635952/1‑140 (MQ=255)                            aaCAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTg                                                                                                     >  2:966644/1‑140 (MQ=255)                            aaCAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTg                                                                                                     >  1:86524/1‑140 (MQ=255)                              cAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCATCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTc                                                                                                             <  2:981463/130‑1 (MQ=255)                              cAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTc                                                                                                             >  1:981463/1‑130 (MQ=255)                              cAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGa                                                                                                   >  2:337252/1‑140 (MQ=255)                               aGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAg                                                                                                  <  2:1031568/140‑1 (MQ=255)                                 gTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAgcg                                                                                                <  2:349617/140‑1 (MQ=255)                                 gTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAgcg                                                                                                >  2:509730/1‑140 (MQ=255)                                    gCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAgcgc                                                                                               <  1:709799/138‑1 (MQ=255)                                    gCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAgcgc                                                                                               >  2:709799/1‑138 (MQ=255)                                         cgcgCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCAt                                                                                        <  2:1023091/140‑1 (MQ=255)                                           cgcAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTg                                                                                      >  2:711817/1‑140 (MQ=255)                                            gcAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTgg                                                                                     <  1:966644/140‑1 (MQ=255)                                             cAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGgc                                                                                    >  2:746117/1‑140 (MQ=255)                                                 aGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCgg                                                                                <  1:588440/140‑1 (MQ=255)                                                 aGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCgg                                                                                <  1:150656/140‑1 (MQ=255)                                                                  gtgtTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCa                                                               <  1:509730/140‑1 (MQ=255)                                                                     ttGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACg                                                            >  1:864003/1‑140 (MQ=255)                                                                        ccGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTg                                                         >  1:195833/1‑140 (MQ=255)                                                                            aTGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCt                                                     >  2:942212/1‑140 (MQ=255)                                                                               accaccTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAg                                                  >  1:797244/1‑140 (MQ=255)                                                                               accaccTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAg                                                  >  2:1009272/1‑140 (MQ=255)                                                                                  accTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAGGtt                                               >  1:144617/1‑140 (MQ=255)                                                                                    cTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAGGTTCg                                             >  2:554049/1‑140 (MQ=255)                                                                                       cGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAGGTTCGAcc                                          >  2:474014/1‑140 (MQ=255)                                                                                          cACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAGGTTCGACCGCa                                       >  1:1011284/1‑140 (MQ=255)                                                                                            ccTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAGGTTCGACCGCAGc                                     <  1:711817/140‑1 (MQ=255)                                                                                             cTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAGGTTCGACCGCAGCt                                    <  1:337252/140‑1 (MQ=255)                                                                                                      cGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAGGTTCGACCGCAGCTACCGTTTCa                           >  2:649358/1‑140 (MQ=255)                                                                                                       gAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAGCATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAGGTTCGACCGCAGCTACCGTTTCaa                          >  2:680523/1‑140 (MQ=255)                                                                                                       gAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAGGTTCGACCGCAGCTACCGTTTCaa                          >  1:164663/1‑140 (MQ=255)                                                                                                          ggCAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAGGTTCGACCGCAGCTACCGTTTCAACtt                       <  1:942212/140‑1 (MQ=255)                                                                                                            cAAAAGTAGGGCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAGGTTCGACCGCAGCTACCGTTTCAACTTct                     <  2:144617/140‑1 (MQ=255)                                                                                                                     ggCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAGGTTCGACCGCAGCTACCGTTTCAACTTCTGCTGCGACt            <  1:474014/140‑1 (MQ=255)                                                                                                                     ggCGCTGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAGGTTCGACCGCAGCTACCGTTTCAACTTCTGCTGCGACt            <  2:164663/140‑1 (MQ=255)                                                                                                                          tGCCAGTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAGGTTCGACCGCAGCTACCGTTTCAACTTCTGCTGCGACTTCAGc       >  1:910047/1‑140 (MQ=255)                                                                                                                               gTCACTGTGATGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAGGTTCGACCGCAGCTACCGTTTCAACTTCTGCTGCGACTTCAGCGacta  <  2:1063215/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  TTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGACGCGGTGCCTCCGGAACATATTCCGGTGCTGGAGCGCGCGTCATTGGCGCGGTAGCGTGGTTATGCTCAACGGTGACCTCAGGTTCGACCGCAGCTACCGTTTCAACTTCTGCTGCGACTTCAGCGACTA  >  NC_000913/1141161‑1141426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑