Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,159,499	A→G	Y685H (TAC→CAC)	fhuE ←	ferric coprogen/ferric rhodotorulic acid outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,159,499	0	A	G	100.0%	214.6 / NA	59	Y685H (TAC→CAC)	fhuE	ferric coprogen/ferric rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/27);  total (32/27)

TCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGC  >  NC_000913/1159362‑1159632                                                                                                                                          |                                                                                                                                      tCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGc                                                                                                                                     <  1:709174/140‑1 (MQ=255)     aaTTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGt                                                                                                                                 >  1:98465/1‑140 (MQ=255)          aTACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCgg                                                                                                                                   <  2:931663/133‑1 (MQ=255)          aTACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCgg                                                                                                                                   >  1:931663/1‑133 (MQ=255)            aCGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAgaga                                                                                                                          >  2:15126/1‑140 (MQ=255)               tGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATcc                                                                                                                       >  2:897341/1‑140 (MQ=255)                 ccGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATccac                                                                                                                     <  1:1020456/140‑1 (MQ=255)                    gTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAg                                                                                                                  >  2:206154/1‑140 (MQ=255)                     tAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAgc                                                                                                                 <  1:1074795/140‑1 (MQ=255)                      aaTGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAgcg                                                                                                                <  1:206154/140‑1 (MQ=255)                           tGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAg                                                                                                           <  2:98465/140‑1 (MQ=255)                               aTTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTAc                                                                                                       >  2:958852/1‑140 (MQ=255)                                   cgcgGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAgcg                                                                                                                >  2:49053/1‑127 (MQ=255)                                   cgcgGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAgcg                                                                                                                <  1:49053/127‑1 (MQ=255)                                    gcgGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGc                                                                                                  >  2:17929/1‑140 (MQ=255)                                          tgCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGgcg                                                                                            <  2:19200/140‑1 (MQ=255)                                                aGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAg                                                                                      <  2:4613/140‑1 (MQ=255)                                                 gACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGa                                                                                        >  2:1037442/1‑137 (MQ=255)                                                 gACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGa                                                                                        <  1:1037442/137‑1 (MQ=255)                                                  aCGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGt                                                                                    >  2:335527/1‑140 (MQ=255)                                                  aCGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGt                                                                                    >  2:582426/1‑140 (MQ=255)                                                  aCGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGt                                                                                    >  1:64347/1‑140 (MQ=255)                                                  aCGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGt                                                                                    <  2:64347/140‑1 (MQ=255)                                                    gATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAg                                                                                      <  1:362855/136‑1 (MQ=255)                                                    gATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAg                                                                                      >  2:362855/1‑136 (MQ=255)                                                    gATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGc                                                                                  >  2:7626/1‑140 (MQ=255)                                                      tAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCa                                                                                >  1:77244/1‑140 (MQ=255)                                                              tCCGCGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCGCCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGt                                                                        <  2:721785/140‑1 (MQ=255)                                                                cACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCa                                                                      <  1:7626/140‑1 (MQ=255)                                                                cACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCa                                                                      <  1:897341/140‑1 (MQ=255)                                                                   gTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACgg                                                                   <  1:582426/140‑1 (MQ=255)                                                                    ttGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGgt                                                                  <  2:267325/140‑1 (MQ=255)                                                                      ggTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACgg                                                                   <  1:469666/137‑1 (MQ=255)                                                                      ggTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACgg                                                                   >  2:469666/1‑137 (MQ=255)                                                                          tCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGt                                                            >  2:25026/1‑140 (MQ=255)                                                                            gTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGtat                                                          >  1:676328/1‑140 (MQ=255)                                                                             tAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGtata                                                         >  1:422974/1‑140 (MQ=255)                                                                               ggTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATaca                                                       >  1:987189/1‑140 (MQ=255)                                                                               ggTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATaca                                                       >  2:281033/1‑140 (MQ=255)                                                                                 ttttGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACAcg                                                     >  1:726260/1‑140 (MQ=255)                                                                                  tttGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACAcgc                                                    >  1:86450/1‑140 (MQ=255)                                                                                    tGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGa                                                  >  2:852491/1‑140 (MQ=255)                                                                                         aaCAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTg                                             <  1:852491/140‑1 (MQ=255)                                                                                         aaCAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTg                                             <  1:335527/140‑1 (MQ=255)                                                                                         aaCAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTg                                             <  2:86450/140‑1 (MQ=255)                                                                                         aaCAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTg                                             <  2:77244/140‑1 (MQ=255)                                                                                          aCAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGc                                            <  1:958852/140‑1 (MQ=255)                                                                                          aCAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGc                                            <  1:17929/140‑1 (MQ=255)                                                                                            aGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCa                                          <  2:1057202/140‑1 (MQ=255)                                                                                            aGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCa                                          <  2:405177/140‑1 (MQ=255)                                                                                                        ttCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACaccacc                              >  1:578270/1‑140 (MQ=255)                                                                                                        ttCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACaccacc                              >  1:1028256/1‑140 (MQ=255)                                                                                                        ttCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACaccacc                              >  1:171245/1‑140 (MQ=255)                                                                                                        ttCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACaccacc                              >  1:346496/1‑140 (MQ=255)                                                                                                        ttCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACaccacc                              >  2:441497/1‑140 (MQ=255)                                                                                                        ttCCCCTGTAACGAGAAG‑TTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCAccg                             >  1:83539/1‑140 (MQ=255)                                                                                                                     agaAGTTTTTCGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAAc                 >  2:314387/1‑140 (MQ=255)                                                                                                                               cGTCACCTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGa       <  2:83539/140‑1 (MQ=255)                                                                                                                                    ccTGGTGGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGc  >  1:441052/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      TCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGC  >  NC_000913/1159362‑1159632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑