Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,217,888	A→G	G187G (GGA→GGG)	pdeG →	putative c‑di‑GMP phosphodiesterase PdeG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,217,888	0	A	G	100.0%	276.3 / NA	78	G187G (GGA→GGG)	pdeG	putative c‑di‑GMP phosphodiesterase PdeG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/40);  total (38/40)

ATACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAATATTTATTCTGTTAAT  >  NC_000913/1217752‑1218019                                                                                                                                         |                                                                                                                                    aTACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTTGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCAt                                                                                                                                  <  1:15749/140‑1 (MQ=255) aTACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCAt                                                                                                                                  <  2:290118/140‑1 (MQ=255) aTACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCAt                                                                                                                                  <  2:247631/140‑1 (MQ=255) aTACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCAt                                                                                                                                  <  1:1061704/140‑1 (MQ=255) aTACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCAt                                                                                                                                  <  2:843377/140‑1 (MQ=255) aTACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCAt                                                                                                                                  <  1:1079167/140‑1 (MQ=255) aTACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCAt                                                                                                                                  <  1:85126/140‑1 (MQ=255) aTACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCAt                                                                                                                                  <  1:650850/140‑1 (MQ=255) aTACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCAt                                                                                                                                  <  1:640398/140‑1 (MQ=255) aTACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCAt                                                                                                                                  <  1:536571/140‑1 (MQ=255)     gcgcATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATc                                                                                                                              <  1:1033356/140‑1 (MQ=255)        cATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCt                                                                                                                           >  1:604232/1‑140 (MQ=255)            ttGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATAc                                                                                                                       <  2:887726/140‑1 (MQ=255)                         gCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCAc                                                                                                          >  2:875504/1‑140 (MQ=255)                              cAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCcac                                                                                                     <  2:293022/140‑1 (MQ=255)                               aGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCcaca                                                                                                    <  2:94484/140‑1 (MQ=255)                                 cATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACaaa                                                                                                  >  1:477101/1‑140 (MQ=255)                                     ttCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAatat                                                                                              <  2:972624/140‑1 (MQ=255)                                       cGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCa                                                                                            >  1:273085/1‑140 (MQ=255)                                         tGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCAtt                                                                                          >  2:924412/1‑140 (MQ=255)                                         tGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCAtt                                                                                          >  1:571314/1‑140 (MQ=255)                                           gggCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTc                                                                                        >  1:616247/1‑140 (MQ=255)                                              cACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACt                                                                                     >  2:861566/1‑140 (MQ=255)                                              cACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACt                                                                                     >  2:930624/1‑140 (MQ=255)                                               aCTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACta                                                                                    <  1:930624/140‑1 (MQ=255)                                                cTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACtat                                                                                   <  1:336374/140‑1 (MQ=255)                                                cTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACtat                                                                                   <  1:968263/140‑1 (MQ=255)                                                cTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACtat                                                                                   <  2:616247/140‑1 (MQ=255)                                                          ccTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAAc                                                                         >  1:103882/1‑140 (MQ=255)                                                           cTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAAcc                                                                        >  1:453025/1‑140 (MQ=255)                                                           cTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAAcc                                                                        <  1:875504/140‑1 (MQ=255)                                                              gAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTACCccgc                                                                     <  2:273085/140‑1 (MQ=255)                                                              gAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACccgc                                                                     >  2:1014437/1‑140 (MQ=255)                                                               aaaGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACccgcc                                                                    >  1:835267/1‑140 (MQ=255)                                                                 aGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCcgccgc                                                                  <  1:861566/140‑1 (MQ=255)                                                                   gggTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCa                                                                <  2:604232/140‑1 (MQ=255)                                                                    ggTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCAc                                                               >  2:507344/1‑140 (MQ=255)                                                                       aaGGTATGTATTACGCGTGGCGGATAACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATCCCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCActct                                                            <  2:648782/140‑1 (MQ=255)                                                                           tatgtttCACGCGTGGCGGATGACATTATGGGCCCAACGGTTGATGTGATGACGCTTAATGGGCATTACCCCTATACCGAGAGGGTTCCCTCCAAAAAATATCTTTTCACTATTATCTTAACCCCGCCGCCACTCTTTAg                                                        <  2:539014/140‑1 (MQ=255)                                                                           tatgtatTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAg                                                        <  2:153666/140‑1 (MQ=255)                                                                           tatgtatTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAg                                                        <  2:103882/140‑1 (MQ=255)                                                                           tatgtatTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAg                                                        <  2:571314/140‑1 (MQ=255)                                                                             tgtatTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAg                                                        >  2:234007/1‑138 (MQ=255)                                                                             tgtatTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAg                                                        >  2:350050/1‑138 (MQ=255)                                                                             tgtatTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAg                                                        <  1:350050/138‑1 (MQ=255)                                                                             tgtatTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAg                                                        <  1:234007/138‑1 (MQ=255)                                                                              gtatTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCTTATACCGAGAAGGTTCACTCCAAAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAg                                                        <  1:215811/137‑1 (MQ=255)                                                                              gtatTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAg                                                        >  2:215811/1‑137 (MQ=255)                                                                              gtatTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCtt                                                     >  1:539014/1‑140 (MQ=255)                                                                                 ttACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTa                                                  <  1:747551/140‑1 (MQ=255)                                                                                    cgcgTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAg                                               >  2:83010/1‑140 (MQ=255)                                                                                    cgcgTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAg                                               >  1:155215/1‑140 (MQ=255)                                                                                    cgcgTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAg                                               >  1:455801/1‑140 (MQ=255)                                                                                    cgcgTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAg                                               >  2:349076/1‑140 (MQ=255)                                                                                    cgcgTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATCATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAg                                               >  2:898958/1‑140 (MQ=255)                                                                                    cgcgTGGCGGATAACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAg                                               >  1:547943/1‑140 (MQ=255)                                                                                     gcgTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGa                                              >  2:660553/1‑140 (MQ=255)                                                                                     gcgTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGa                                              >  2:629833/1‑140 (MQ=255)                                                                                       gTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCActct                                                            >  1:1023038/1‑124 (MQ=255)                                                                                       gTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCActct                                                            <  2:1023038/124‑1 (MQ=255)                                                                                         ggCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTa                                          >  2:408024/1‑140 (MQ=255)                                                                                         ggCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTa                                          >  1:569505/1‑140 (MQ=255)                                                                                         ggCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTa                                          >  2:903927/1‑140 (MQ=255)                                                                                           cGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATc                                        >  1:801843/1‑140 (MQ=255)                                                                                               taaCATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATa                                    <  2:547943/138‑1 (MQ=255)                                                                                               tGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATCCCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTACCCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATa                                    <  1:529324/140‑1 (MQ=255)                                                                                               tGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATa                                    <  1:903927/140‑1 (MQ=255)                                                                                                      attGGACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCtt                             <  2:569505/140‑1 (MQ=255)                                                                                                         ggACCAACGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTACCCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTgg                          <  2:97650/140‑1 (MQ=255)                                                                                                               aCGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGAtttt                    <  1:660553/140‑1 (MQ=255)                                                                                                               aCGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGAtttt                    >  1:619990/1‑140 (MQ=255)                                                                                                               aCGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGAtttt                    >  1:610314/1‑140 (MQ=255)                                                                                                                cGGGTGATGTGATGACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTa                   >  2:1011752/1‑140 (MQ=255)                                                                                                                    tgatgtgatgACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCCCCCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAatat               <  1:423050/140‑1 (MQ=255)                                                                                                                    tgatgtgatgACGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAatat               >  2:912693/1‑140 (MQ=255)                                                                                                                         tgatgaCGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAAtatttatt          <  1:507344/140‑1 (MQ=255)                                                                                                                          gatgaCGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAATATTTATTc         <  1:912693/140‑1 (MQ=255)                                                                                                                           atgaCGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAATATTTATTCt        >  1:863490/1‑140 (MQ=255)                                                                                                                            tgaCGCTTAATGGGCATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAATATTTATTCTg       >  1:1063591/1‑140 (MQ=255)                                                                                                                               cGCTTAATGGGCATCATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAATATTTATTCTGTTa    <  1:898958/140‑1 (MQ=255)                                                                                                                                 cTTAATGGGCATTATCCCTATCCCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAATATTTATTCTGTTAAt  >  2:181818/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    ATACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAATATTTATTCTGTTAAT  >  NC_000913/1217752‑1218019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑