Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,371,613	G→A	R466R (AGG→AGA)	ycjM →	glucosylglycerate phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,371,613	0	G	A	100.0%	223.5 / NA	63	R466R (AGG→AGA)	ycjM	glucosylglycerate phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/30);  total (33/30)

ATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGT  >  NC_000913/1371476‑1371748                                                                                                                                          |                                                                                                                                        atatatatTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACa                                                                                                                                       >  2:1019130/1‑140 (MQ=255) atatatatTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACa                                                                                                                                       <  1:766817/140‑1 (MQ=255)   atatatTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAGCTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATg                                                                                                                                     <  2:684651/140‑1 (MQ=255)   atatatTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATg                                                                                                                                     >  2:139167/1‑140 (MQ=255)   atatatTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATg                                                                                                                                     <  2:381737/140‑1 (MQ=255)   atatatTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATg                                                                                                                                     >  1:740572/1‑140 (MQ=255)    tatatTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGc                                                                                                                                    <  2:562579/140‑1 (MQ=255)    tatatTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGc                                                                                                                                    <  1:139167/140‑1 (MQ=255)     atatTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCg                                                                                                                                   <  1:693477/140‑1 (MQ=255)      tatTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCgg                                                                                                                                  <  1:753298/140‑1 (MQ=255)      tatTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCgg                                                                                                                                  <  2:189017/140‑1 (MQ=255)      tatTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCgg                                                                                                                                  <  2:529869/140‑1 (MQ=255)         tCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGtat                                                                                                                               >  2:1065963/1‑140 (MQ=255)             aGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCa                                                                                                                           >  2:33605/1‑140 (MQ=255)                 ttCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAg                                                                                                                       >  1:631093/1‑140 (MQ=255)                 ttCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAg                                                                                                                       >  1:674174/1‑140 (MQ=255)                  tCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGt                                                                                                                      >  2:434608/1‑140 (MQ=255)                  tCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGt                                                                                                                      >  1:724290/1‑140 (MQ=255)                  tCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGt                                                                                                                      >  2:967105/1‑140 (MQ=255)                       gCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTcg                                                                                                                 >  2:941713/1‑140 (MQ=255)                        cTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTcgc                                                                                                                >  1:910883/1‑140 (MQ=255)                         tCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTcgcg                                                                                                               >  1:774623/1‑140 (MQ=255)                         tCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTcgcg                                                                                                               <  2:613272/140‑1 (MQ=255)                         tCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTcgcg                                                                                                               <  2:774623/140‑1 (MQ=255)                         tCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTcgcg                                                                                                               >  1:201200/1‑140 (MQ=255)                         tCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTcgcg                                                                                                               <  1:1019130/140‑1 (MQ=255)                            cgTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGttt                                                                                                            >  2:3772/1‑140 (MQ=255)                            cgTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGttt                                                                                                            >  1:960002/1‑140 (MQ=255)                                           ggTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTtcgtcg                                                                                             <  1:119440/140‑1 (MQ=255)                                           ggTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTtcgtcg                                                                                             <  1:434608/140‑1 (MQ=255)                                                    aaaCTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATaa                                                                                    <  2:669265/140‑1 (MQ=255)                                                              ataACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCAt                                                                          <  2:971053/140‑1 (MQ=255)                                                                 aCCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCg                                                                       >  2:787754/1‑140 (MQ=255)                                                                     tGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGata                                                                   <  2:960002/140‑1 (MQ=255)                                                                      gCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGataa                                                                  >  1:44475/1‑140 (MQ=255)                                                                      gCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCAT‑AGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATc                                                                         >  1:844902/1‑132 (MQ=255)                                                                      gCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCAT‑AGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATc                                                                         <  2:844902/132‑1 (MQ=255)                                                                         aTTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGAtaataa                                                               >  2:111655/1‑140 (MQ=255)                                                                            aaCCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAAttt                                                            >  1:812578/1‑140 (MQ=255)                                                                                  aaaaaaTATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCAt                                                      >  1:266984/1‑140 (MQ=255)                                                                                     aaaTATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGa                                                   <  1:33605/140‑1 (MQ=255)                                                                                                tAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAAtt                                        <  1:870679/140‑1 (MQ=255)                                                                                                       aTAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt                                 <  2:910883/140‑1 (MQ=255)                                                                                                        tAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTa                                >  2:337010/1‑140 (MQ=255)                                                                                                        tAACCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTa                                >  2:214674/1‑140 (MQ=255)                                                                                                          aCCCGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAAt                              >  1:900444/1‑140 (MQ=255)                                                                                                           cccGAGAACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATg                             <  2:631093/140‑1 (MQ=255)                                                                                                               agaACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTa                         >  2:471817/1‑140 (MQ=255)                                                                                                               agaACTGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTa                         <  2:812578/140‑1 (MQ=255)                                                                                                                    tGAACGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCa                    <  1:787754/140‑1 (MQ=255)                                                                                                                      aaCGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCaag                  >  1:219966/1‑140 (MQ=255)                                                                                                                      aaCGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCaag                  >  2:115771/1‑140 (MQ=255)                                                                                                                      aaCGATGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCaag                  >  1:474444/1‑140 (MQ=255)                                                                                                                           tGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTa             >  2:606381/1‑140 (MQ=255)                                                                                                                           tGAAGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTa             >  2:385594/1‑140 (MQ=255)                                                                                                                              aGCTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACg          <  1:385594/140‑1 (MQ=255)                                                                                                                                cTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCt        >  1:856789/1‑140 (MQ=255)                                                                                                                                cTACATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCt        >  1:749314/1‑140 (MQ=255)                                                                                                                                  aCATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGCGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGa      <  2:856789/140‑1 (MQ=255)                                                                                                                                  aCATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGa      <  2:749314/140‑1 (MQ=255)                                                                                                                                  aCATTAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGa      <  1:3772/140‑1 (MQ=255)                                                                                                                                      tAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGt  <  1:471817/140‑1 (MQ=255)                                                                                                                                      tAAGACATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGt  <  2:432587/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        ATATATATTCAAAGTATTCTTGGCTCGCGTAATGATTACGCAGGTGTCGAAAAACTCGGATATAACCGTGCGATTAACCGTAAAAAATATCACAGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGT  >  NC_000913/1371476‑1371748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑