Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,496,794	C→T	intergenic (+163/‑62)	ydcJ → / → opgD	DUF1338 domain‑containing protein YdcJ/glucan biosynthesis protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,496,794	0	C	T	100.0%	127.4 / NA	36	intergenic (+163/‑62)	ydcJ/opgD	DUF1338 domain‑containing protein YdcJ/glucan biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/17);  total (19/17)

TGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCATTGCTTCTCT  >  NC_000913/1496660‑1496929                                                                                                                                       |                                                                                                                                        tGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGcc                                                                                                                                    >  1:1018627/1‑140 (MQ=255)       aTTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTAAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGaa                                                                                                                              <  2:544248/140‑1 (MQ=255)             cAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGAc                                                                                                                        <  1:56392/140‑1 (MQ=255)                 cGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAgc                                                                                                                    >  2:124183/1‑140 (MQ=255)                    aTCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGcgcg                                                                                                                 <  1:374616/140‑1 (MQ=255)                    aTCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGcgcg                                                                                                                 <  1:550037/140‑1 (MQ=255)                        ggCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCACCGCGGGAc                                                                                                             >  1:508426/1‑140 (MQ=255)                              tCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTca                                                                                                       <  2:437880/140‑1 (MQ=255)                                  cacGACATTAAAAAAAAGCCGAATTATGGCTATTCTTTCAGTTCAACCATTGGATTTATAATAAATATTGTCTAGAGTGGGCGGTCATAAATAAGCCCTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCacaac                                                                                                   <  2:508426/138‑1 (MQ=255)                                             aaaaaTGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGAc                                                                                        <  2:565203/140‑1 (MQ=255)                                                    ccGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACttt                                                                                 <  2:557978/140‑1 (MQ=255)                                                       aTTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAg                                                                              >  2:893156/1‑140 (MQ=255)                                                       aTTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAg                                                                              <  2:1018627/140‑1 (MQ=255)                                                                     tctcAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGa                                                                <  1:124183/140‑1 (MQ=255)                                                                           ttCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTa                                                                           >  2:1032525/1‑123 (MQ=255)                                                                           ttCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTa                                                                           <  1:1032525/123‑1 (MQ=255)                                                                              aacaaTTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATttatt                                                       >  1:479726/1‑140 (MQ=255)                                                                              aacaaTTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATttatt                                                       <  2:479726/140‑1 (MQ=255)                                                                                     ggATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGtt                                                >  2:853625/1‑140 (MQ=255)                                                                                     ggATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGtt                                                >  2:53234/1‑140 (MQ=255)                                                                                                aaataTTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATg                                     >  1:752018/1‑140 (MQ=255)                                                                                                    atTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGgccg                                 <  1:53234/140‑1 (MQ=255)                                                                                                        tCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCgt                             >  1:800970/1‑140 (MQ=255)                                                                                                        tCTAGAGTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCgt                             >  2:383393/1‑140 (MQ=255)                                                                                                             agTGCGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGGTAACGATTTATTAAAGTTTCAATGGCTATGGCCGCCGtgtggg                        >  2:530094/1‑138 (MQ=255)                                                                                                             agTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCg                        >  2:442102/1‑140 (MQ=255)                                                                                                             agTGAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCg                        >  1:755487/1‑140 (MQ=255)                                                                                                                gAGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTa                     >  2:191853/1‑140 (MQ=255)                                                                                                                 aGCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTAc                    >  2:765368/1‑140 (MQ=255)                                                                                                                  gCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTAcc                   >  1:487138/1‑140 (MQ=255)                                                                                                                  gCGGTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTAcc                   >  2:483821/1‑140 (MQ=255)                                                                                                                    ggTCATAAATAAGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAg                 <  1:442102/140‑1 (MQ=255)                                                                                                                              aaGCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCATTGCt       <  1:483821/140‑1 (MQ=255)                                                                                                                                gCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCATTGCTtc     <  2:133873/140‑1 (MQ=255)                                                                                                                                gCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCATTGCTtc     >  1:133873/1‑140 (MQ=255)                                                                                                                                gCACTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCATTGCTtc     >  2:85231/1‑140 (MQ=255)                                                                                                                                   cTTTTTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCATTGCTTctct  <  1:765368/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TGCCTGATTTTACAAACGAATCAGGCTCATCCCATCGACATAAAAAAAATGCCGATTTATGCATATTCTCTCAGTTCAACAATTGGATTATTAATAAATATTGTCTAGAGTGAGCGGTCATAAATAAGCACTTTCTTGCCGCTGAAAACGACCAGCGCGGGACCATTCACAACACCAGAAGGACTCACTTTCAGGTATGGATCGTAGACGATTTATTAAAGGTTCAATGGCTATGGCCGCCGTGTGCGGTACCAGCGGCATTGCTTCTCT  >  NC_000913/1496660‑1496929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑