Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,605,789	A→G	T247A (ACG→GCG)	lsrB →	Autoinducer‑2 ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,605,789	0	A	G	100.0%	66.1 / NA	20	T247A (ACG→GCG)	lsrB	Autoinducer‑2 ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/11);  total (9/11)

ACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCG  >  NC_000913/1605653‑1605924                                                                                                                                         |                                                                                                                                        gacagccGCAGAAGGAATTTTAAAAGGGTATAGCGATCTCGAGCCCATTATCGCCCCCGTTGCAATCGCCATCCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTgcgc                                                                                                                                      <  2:703976/135‑1 (MQ=255)              ggAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATgcg                                                                                                                         <  1:180215/140‑1 (MQ=255)                aaTATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATGCGcc                                                                                                                       <  2:648449/140‑1 (MQ=255)                 atatTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATgctgtc                                                                                                                      >  1:703976/1‑136 (MQ=255)                         aGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATGCGCCCGTATGTag                                                                                                              >  2:267637/1‑140 (MQ=255)                                  cGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCa                                                                                                     <  1:267637/140‑1 (MQ=255)                                                   tCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGc                                                                                    <  2:469926/140‑1 (MQ=255)                                                          cGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTggg                                                                             >  2:582885/1‑140 (MQ=255)                                                           gATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGa                                                                            <  1:555338/140‑1 (MQ=255)                                                                    gCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTgg                                                                                     >  1:531323/1‑122 (MQ=255)                                                                    gCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTgg                                                                                     <  2:531323/122‑1 (MQ=255)                                                                        tGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCaa                                                               >  2:890037/1‑140 (MQ=255)                                                                                           cGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATg                                            >  2:1057624/1‑140 (MQ=255)                                                                                           cGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATg                                            >  2:688898/1‑140 (MQ=255)                                                                                            gCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGt                                           <  1:974544/140‑1 (MQ=255)                                                                                               gAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTcgc                                        <  1:890037/140‑1 (MQ=255)                                                                                                          aaaTGACAAAGTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCattat                             >  1:499417/1‑140 (MQ=255)                                                                                                          aaaTGACAAAGTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCattat                             >  1:450150/1‑140 (MQ=255)                                                                                                                    gTAGCGATTGTCGGATTCAGTGCGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAgg                   <  1:319681/140‑1 (MQ=255)                                                                                                                     tAGCGATTGTCGGATTCAGTGCGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGa                  <  2:191551/140‑1 (MQ=255)                                                                                                                                     cAGTGCGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCg  <  2:561426/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        ACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGCAGAAAACTTGAAAAATGACAAAGTAGCGATTGTCGGATTCAGTACGCCAAATGTGATGCGCCCGTATGTAGAGCGCGGCACGGTGAAAGAATTTGGCCTGTGGGATGTGGTTCAGCAAGGCAAAATTTCAGTGTATGTCGCGGATGCATTATTGAAAAAAGGATCAATGAAAACGGGCG  >  NC_000913/1605653‑1605924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑