Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,690,803	A→G	T318A (ACT→GCT)	ydgA →	conserved protein YdgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,690,803	0	A	G	100.0%	162.1 / NA	45	T318A (ACT→GCT)	ydgA	conserved protein YdgA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/17);  total (28/17)

TAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGTACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGCT  >  NC_000913/1690670‑1690938                                                                                                                                      |                                                                                                                                        tAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGTACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAgg                                                                                                                                   <  1:793698/140‑1 (MQ=255)    ccAACTGGATTACTCGCTAAACAGCCTGAAGGTACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGgcgc                                                                                                                                <  2:561318/140‑1 (MQ=255)       aCTGGATTACTCGCTAAACAGCCTGAAGGTACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGgc                                                                                                                                  >  1:230987/1‑135 (MQ=255)       aCTGGATTACTCGCTAAACAGCCTGAAGGTACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGgc                                                                                                                                  <  2:230987/135‑1 (MQ=255)       aCTGGATTACTCGCTAAACAGCCTGAAGGTACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCgctgc                                                                                                                             >  2:1061874/1‑140 (MQ=255)         tGGATTACTCGCTAAACAGCCTGAAGGTACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGctgctg                                                                                                                           >  2:464932/1‑140 (MQ=255)            aTTACTCGCTAAACAGCCTGAAGGTACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGca                                                                                                                        <  1:1061874/140‑1 (MQ=255)                     tAAACAGCCTGAAGGTACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGaa                                                                                                               >  2:748180/1‑140 (MQ=255)                            ccTGAAGGTACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCca                                                                                                        >  1:256871/1‑140 (MQ=255)                            ccTGAAGGTACAGAATCAGGATCTGGGCAGAGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCca                                                                                                        >  2:925879/1‑140 (MQ=255)                                 aGGTACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCaacaac                                                                                                   >  1:697491/1‑140 (MQ=255)                                   gTACAGAATCAGGATCTGGGCAGAGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAAccc                                                                                                 <  1:925879/140‑1 (MQ=255)                                      cAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCcaac                                                                                                      >  1:52802/1‑132 (MQ=255)                                      cAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCcaac                                                                                                      <  2:52802/132‑1 (MQ=255)                                      cAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGa                                                                                              >  2:95172/1‑140 (MQ=255)                                        gAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCAACAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAAc                                                                                            <  2:667238/140‑1 (MQ=255)                                             aGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTAt                                                                                       >  1:880838/1‑140 (MQ=255)                                              ggttCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATc                                                                                      >  2:285414/4‑140 (MQ=255)                                              ggATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATc                                                                                      >  2:841057/1‑140 (MQ=255)                                                   tGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGgag                                                                                 >  2:67428/1‑140 (MQ=255)                                                   tGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGgag                                                                                 >  2:206261/1‑140 (MQ=255)                                                          cGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGa                                                                          <  2:232038/140‑1 (MQ=255)                                                             cAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACgg                                                                       <  2:880838/140‑1 (MQ=255)                                                                                     gATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGc                                               <  1:484397/140‑1 (MQ=255)                                                                                               gAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGaa                                     <  1:67428/140‑1 (MQ=255)                                                                                                aaGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGaaa                                    >  2:826811/1‑140 (MQ=255)                                                                                                 aGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAg                                   >  1:439372/1‑140 (MQ=255)                                                                                                     tGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGa                               >  1:295046/1‑140 (MQ=255)                                                                                                     tGGCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGa                               >  1:570558/1‑140 (MQ=255)                                                                                                      ggCATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGAt                              <  1:206261/140‑1 (MQ=255)                                                                                                        cATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATcc                            >  2:852968/1‑140 (MQ=255)                                                                                                        cATCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATcc                            >  2:422117/1‑140 (MQ=255)                                                                                                         aTCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCg                           <  1:826811/140‑1 (MQ=255)                                                                                                         aTCAGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCg                           <  1:748180/140‑1 (MQ=255)                                                                                                            aGTTTAGCCAGCAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTg                        >  2:1047973/1‑140 (MQ=255)                                                                                                                       cAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATcg              >  1:305349/1‑139 (MQ=255)                                                                                                                       cAATATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATcg              <  2:305349/139‑1 (MQ=255)                                                                                                                        aaTATAACGCGCAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATcgcg            >  1:241602/1‑140 (MQ=255)                                                                                                                                 cgcAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGc   >  2:629866/1‑140 (MQ=255)                                                                                                                                 cgcAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGc   >  1:377281/1‑140 (MQ=255)                                                                                                                                 cgcAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGc   >  1:1045282/1‑140 (MQ=255)                                                                                                                                 cgcAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGc   >  1:439020/1‑140 (MQ=255)                                                                                                                                  gcAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGcgccg         >  2:471905/1‑133 (MQ=255)                                                                                                                                  gcAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGcgccg         <  1:471905/133‑1 (MQ=255)                                                                                                                                  gcAAGCTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGCt  <  1:422117/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        TAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGTACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACAACCCCGAACTTTATCAGGAGAAAGTGACGGAAGCCTTCTTTAGCGCCCTGCCGCTGATGCTGAAAGGCGATCCGGTGATTACTATCGCGCCGCTAAGCT  >  NC_000913/1690670‑1690938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑