Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,925,458	G→A	A7T (GCA→ACA)	yobB →	putative carbon‑nitrogen hydrolase family protein YobB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,925,458	0	G	A	100.0%	240.5 / NA	67	A7T (GCA→ACA)	yobB	putative carbon‑nitrogen hydrolase family protein YobB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/26);  total (41/26)

AGCCCAAACTTAAATAAAACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTG  >  NC_000913/1925322‑1925590                                                                                                                                         |                                                                                                                                     aGCCCAAACTTAAATAAAACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTacag                                                                                                                                   <  2:292511/140‑1 (MQ=255) aGCCCAAACTTAAATAAAACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTA‑TTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTacagc                                                                                                                                  <  2:927876/140‑1 (MQ=255)    ccAAACTTAAATAAAACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTAcagcag                                                                                                                                >  2:387377/1‑140 (MQ=255)     cAAACTTAAATAAAACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACagcagc                                                                                                                               <  2:331188/140‑1 (MQ=255)      aaaCTTAAATAAAACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAgcg                                                                                                                              <  1:284316/140‑1 (MQ=255)                aaaaCTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGc                                                                                                                    <  1:625939/140‑1 (MQ=255)                               ttACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACctcac                                                                                                     >  1:273508/1‑140 (MQ=255)                                tACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACctcact                                                                                                    >  1:861723/1‑140 (MQ=255)                                  cacTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACctcactca                                                                                                  <  1:1010021/140‑1 (MQ=255)                                    cTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCAcc                                                                                                <  1:77310/140‑1 (MQ=255)                                       tCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACctc                                                                                                       <  2:628272/130‑1 (MQ=255)                                       tCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACctc                                                                                                       >  1:628272/1‑130 (MQ=255)                                       tCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGaa                                                                                             >  1:475206/1‑140 (MQ=255)                                       tCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGaa                                                                                             >  1:187122/1‑140 (MQ=255)                                       tCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGaa                                                                                             >  1:1064982/1‑140 (MQ=255)                                       tCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGaa                                                                                             >  1:536968/1‑140 (MQ=255)                                         ttACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACa                                                                                           >  1:894742/1‑140 (MQ=255)                                             aTAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCACAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTa                                                                                       >  1:140159/1‑140 (MQ=255)                                                 cGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGa                                                                                              >  1:222682/1‑129 (MQ=255)                                                 cGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGa                                                                                              <  2:222682/129‑1 (MQ=255)                                                 cGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCtc                                                                                   >  1:863043/1‑140 (MQ=255)                                                 cGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCtc                                                                                   <  1:89705/140‑1 (MQ=255)                                                 cGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCtc                                                                                   >  2:89705/1‑140 (MQ=255)                                                 cGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCtc                                                                                   <  2:426281/140‑1 (MQ=255)                                                 cGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCtc                                                                                   >  2:507495/1‑140 (MQ=255)                                                 cGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCtc                                                                                   >  2:51085/1‑140 (MQ=255)                                                 cGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCtc                                                                                   >  2:760133/1‑140 (MQ=255)                                                 cGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCtc                                                                                   >  2:599533/1‑140 (MQ=255)                                                 cGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCtc                                                                                   >  2:689563/1‑140 (MQ=255)                                                 cGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCtc                                                                                   >  1:428054/1‑140 (MQ=255)                                                 cGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCtc                                                                                   >  1:708231/1‑140 (MQ=255)                                                 cGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCtc                                                                                   >  1:568558/1‑140 (MQ=255)                                                 cGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCtc                                                                                   <  1:599533/140‑1 (MQ=255)                                                 cGCCTGCTAAATTATGAGTATTTTCAAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCtc                                                                                   >  1:840039/1‑140 (MQ=255)                                                   ccTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCtcat                                                                                 >  1:463318/1‑140 (MQ=255)                                                   ccTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCtcat                                                                                 <  2:861723/140‑1 (MQ=255)                                                         aaaTTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTg                                                                           <  1:507495/140‑1 (MQ=255)                                                                 aGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTtgt                                                                   >  1:654172/1‑140 (MQ=255)                                                                            aaCCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGgccgccg                                                        <  2:536968/140‑1 (MQ=255)                                                                              ccGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGgccgccgcc                                                      <  1:387377/140‑1 (MQ=255)                                                                              ccGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGgccgccgcc                                                      <  2:251239/140‑1 (MQ=255)                                                                               cGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGGGTTTGTGAGGGCCGCCGCCa                                                     >  1:509357/1‑140 (MQ=255)                                                                               cGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCa                                                     <  2:182599/140‑1 (MQ=255)                                                                                gCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAg                                                    >  2:752778/1‑140 (MQ=255)                                                                                 cACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAg                                                    >  2:970359/1‑139 (MQ=255)                                                                                 cACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAg                                                    <  1:970359/139‑1 (MQ=255)                                                                                  aCTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGc                                                  <  2:840039/140‑1 (MQ=255)                                                                                   cTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGca                                                 >  2:1033037/1‑140 (MQ=255)                                                                                     cATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGcaac                                               <  2:226792/140‑1 (MQ=255)                                                                                      aTAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGcaaca                                              >  1:1048813/1‑140 (MQ=255)                                                                                      aTAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGcaaca                                              >  2:477731/1‑140 (MQ=255)                                                                                            tGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCa                                        >  2:203515/1‑140 (MQ=255)                                                                                              cAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGc                                      >  1:524230/1‑140 (MQ=255)                                                                                                gTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTg                                    >  1:166462/1‑140 (MQ=255)                                                                                                     tttGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGt                               >  1:143348/1‑140 (MQ=255)                                                                                                                aGTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGc                    <  2:866161/140‑1 (MQ=255)                                                                                                                 gTCATTATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGct                   <  1:752778/140‑1 (MQ=255)                                                                                                                     ttATGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGctttct               <  1:477731/140‑1 (MQ=255)                                                                                                                        tGTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTa            >  1:742221/1‑140 (MQ=255)                                                                                                                         gTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTAc           <  1:1033037/140‑1 (MQ=255)                                                                                                                         gTCGTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTAc           >  2:473115/1‑140 (MQ=255)                                                                                                                            gTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTgg        <  2:654172/140‑1 (MQ=255)                                                                                                                            gTTCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTgg        >  2:988395/1‑140 (MQ=255)                                                                                                                             ttCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTggg       <  2:273508/140‑1 (MQ=255)                                                                                                                             ttCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTggg       <  2:143348/140‑1 (MQ=255)                                                                                                                             ttCTGGAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTggg       >  1:421063/1‑140 (MQ=255)                                                                                                                             ttCTGGAAAGTTACAGCAGCACAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTggg       >  2:480719/1‑140 (MQ=255)                                                                                                                                  gAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGtgtg  >  2:53458/1‑140 (MQ=255)                                                                                                                                  gAAAGTTACAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGtgtg  >  1:614725/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     AGCCCAAACTTAAATAAAACTTATACAGAGTTACACTTTCTTACATAACGCCTGCTAAATTATGAGTATTTTCTAAACCGCACTCATAATTTGCAGTCATTTTGAAAAGGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTG  >  NC_000913/1925322‑1925590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑