Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,098,169	C→T	A45T (GCT→ACT)	wzzB ←	regulator of length of O‑antigen component of lipopolysaccharide chains

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,098,169	0	C	T	100.0%	145.2 / NA	44	A45T (GCT→ACT)	wzzB	regulator of length of O‑antigen component of lipopolysaccharide chains
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/12);  total (32/12)

TCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCAT  >  NC_000913/2098033‑2098304                                                                                                                                         |                                                                                                                                        tCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAg                                                                                                                                      >  2:12151/1‑140 (MQ=255)     aTACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCa                                                                                                                                  <  2:134328/140‑1 (MQ=255)      tACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCaa                                                                                                                                 <  1:385534/140‑1 (MQ=255)          ttCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGc                                                                                                                             <  2:630638/140‑1 (MQ=255)           tCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGcc                                                                                                                            >  2:438316/1‑140 (MQ=255)           tCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGcc                                                                                                                            >  2:491842/1‑140 (MQ=255)                    ccTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACg                                                                                                                   >  1:21179/1‑140 (MQ=255)                           aTAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAatga                                                                                                            <  1:491842/140‑1 (MQ=255)                              gATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAAtgatga                                                                                                         >  1:258260/1‑140 (MQ=255)                              gATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAAtgatga                                                                                                         >  2:629285/1‑140 (MQ=255)                                       cATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCt                                                                                                >  1:399627/1‑140 (MQ=255)                                        aTGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCtt                                                                                               >  2:652266/1‑140 (MQ=255)                                          ggCATTGTTATAGCCAGCATTTTCCCCCACGTGGGGCTGAGTGATAATTGTTGTTGACGTCCTTTTCTCCTTCGCTACTGCAAAATATCAAATAGTCAGGGAAATAGCCAAAATGACGAAAATGATGATTGTCATCTTgc                                                                                             <  2:244195/140‑1 (MQ=255)                                          ggCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgc                                                                                             >  2:921636/1‑140 (MQ=255)                                          agacaggTTATAGCAAGCATTTTCCCCCCCGTGGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgc                                                                                             <  2:116379/134‑1 (MQ=255)                                           gCATTGTTATAGCCAGCAATTTCCCCCACGTCGGGCTGAGTGAAATTTGCTGTGGACGTCCTTTTCCCCTTCGCTACTGCAAAATACCAAAAAGTCAGGGCAATAGCCACAATGACGGAAATGATGTTTGTCATCTTgcc                                                                                            <  2:258260/140‑1 (MQ=255)                                            cATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgcca                                                                                           <  1:921636/140‑1 (MQ=255)                                              ttGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgccacg                                                                                         >  2:560263/1‑140 (MQ=255)                                               tGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCTTTTCTCCTCCGCTACTGCAAAATATCCAATAGTCAGGGAAATAGCCACAATGACGAAAATGATGATTGTCATCTTgccacgc                                                                                        <  2:1008428/140‑1 (MQ=255)                                               tGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCAAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgccacgc                                                                                        <  1:477737/140‑1 (MQ=255)                                                  tataGCCAGCATTTTGCCCCACGTCGGGCTGAGTAATATTTGCTGTTGCCGTCCTTTCCTCCTTCGCTACGGCAAAATATCAAATAGTCAGGGAAATAGCCAAAATGACGGAAATGATGATTGTCATCTTgccacgccac                                                                                     <  2:250278/140‑1 (MQ=255)                                                    taGCCAGAAATTTGCCCCACGTGGGGCTGAGTGATAATTGCTGTTGACGTCCTTTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGAAATAGCCACAATGACGGAAATGATGTTTGTCATCTTGCCACGCCACaa                                                                                   <  2:495141/140‑1 (MQ=255)                                                        cAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGc                                                                               >  1:512174/1‑140 (MQ=255)                                                         aGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCa                                                                              >  1:751061/1‑140 (MQ=255)                                                         aGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCa                                                                              >  1:250278/1‑140 (MQ=255)                                                           cAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACaa                                                                                   <  2:262845/133‑1 (MQ=255)                                                           cAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACaa                                                                                   >  1:262845/1‑133 (MQ=255)                                                           cAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAAcctgtct                                                                            >  1:116379/1‑134 (MQ=255)                                                           cAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACt                                                                            >  1:92086/1‑140 (MQ=255)                                                           cAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACt                                                                            >  1:480274/1‑140 (MQ=255)                                                           cAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACt                                                                            >  1:852849/1‑140 (MQ=255)                                                           cAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACt                                                                            >  1:802888/1‑140 (MQ=255)                                                           cAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACt                                                                            >  1:364677/1‑140 (MQ=255)                                                                 gCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTaaa                                                                      >  1:646300/1‑140 (MQ=255)                                                                               tGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCAAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTaaatcaatcaaatcaat                                                        <  1:192707/140‑1 (MQ=255)                                                                                 aGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCt                                                      >  2:615233/1‑140 (MQ=255)                                                                                 aGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCt                                                      >  1:285803/1‑140 (MQ=255)                                                                                  gTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTg                                                     >  1:997627/1‑140 (MQ=255)                                                                                  gTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTg                                                     >  1:840569/1‑140 (MQ=255)                                                                                     aTAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTc                                                  <  2:852849/140‑1 (MQ=255)                                                                                     aTAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTc                                                  <  1:615233/140‑1 (MQ=255)                                                                                           gCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTc                                            >  2:863981/1‑140 (MQ=255)                                                                                           gCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTc                                            >  2:133203/1‑140 (MQ=255)                                                                                               ttGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATgg                                        >  2:50943/1‑140 (MQ=255)                                                                                               ttGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATCACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATgg                                        >  1:108989/1‑140 (MQ=255)                                                                                                          ttCTCCTTCGCTACTGCCAAATATCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGa                             <  1:50943/140‑1 (MQ=255)                                                                                                              ccTTCGCTACTGCAAAATATCCAATAGCCGGGGAAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACa                         <  2:155691/140‑1 (MQ=255)                                                                                                                           aaaaTCTCAAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATTTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTa            <  2:219409/139‑1 (MQ=255)                                                                                                                             aaTACCCAATAGGCAGGGCAATAGCCAAAATGAGGGAAATGGTGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTAct          <  1:811185/140‑1 (MQ=255)                                                                                                                               tatCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTActct        <  1:652266/140‑1 (MQ=255)                                                                                                                               tatCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTActct        >  1:257606/1‑140 (MQ=255)                                                                                                                               tatCCAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTActct        <  2:997627/140‑1 (MQ=255)                                                                                                                                  ccAATAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCtcat     >  2:439081/1‑140 (MQ=255)                                                                                                                                    aaTAGTCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCtcatca   >  2:1072003/1‑140 (MQ=255)                                                                                                                                     aTAGTCAGGGAAATACCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTcatcat  <  2:229991/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TCCGATACTTTCGGTGCAGCCTGACCATAGATAACATTCATGGCATTGTTATAGCCAGCAATTTGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTAAATCAATCAAATCAATCTGTTCCGGGTCATGGTTTTGCCCAGAAACATTATTATTTTCTACTCTCATCAT  >  NC_000913/2098033‑2098304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑