Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,300,287	C→T	V823I (GTC→ATC)	napA ←	periplasmic nitrate reductase subunit NapA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,300,287	0	C	T	100.0%	188.7 / NA	53	V823I (GTC→ATC)	napA	periplasmic nitrate reductase subunit NapA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/27);  total (26/27)

CTGTTGTAACCCCAGCGCCACACCCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACCGCGCGTTTC  >  NC_000913/2300153‑2300422                                                                                                                                       |                                                                                                                                        cTGTTGTAACCCCAGCGCCACACCCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCgcgc                                                                                                                                    <  1:3393/140‑1 (MQ=255)       tAACCCCAGCGCCACACCCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATcgcg                                                                                                                                     <  2:328655/133‑1 (MQ=255)       tAACCCCAGCGCCACACCCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATcgcg                                                                                                                                     >  1:328655/1‑133 (MQ=255)       tAACCCCAGCGCCACACCCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCActtct                                                                                                                              >  2:771485/1‑140 (MQ=255)         aCCCCAGCGCCACACCCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTg                                                                                                                            >  2:921127/1‑140 (MQ=255)            ccAGCGCCACACCCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAg                                                                                                                         >  2:991262/1‑140 (MQ=255)              aGCGCCACACCCTCGGCTGCCAGCCCGTCTGCTGTGCGAACGACATCGCGCAGTAAGCGGCGGCGACCATTTGGAGGTTTCGCTGAGCGGGACATAATGGCTTACACCTTCTCCAGTTTGTTCGCGCACTTCTTTAAGTc                                                                                                                       >  2:320717/1‑140 (MQ=255)              aGCGCCACACCCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACGTTCTCCAGTTTGATCGCGCACTTCTTGAAGTc                                                                                                                       >  2:953107/1‑140 (MQ=255)              aGCGCCACACCCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTc                                                                                                                       >  1:1066983/1‑140 (MQ=255)              aGCGCCACACCCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTc                                                                                                                       <  2:22104/140‑1 (MQ=255)              aGCGCCACACCCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTc                                                                                                                       >  1:918858/1‑140 (MQ=255)              aGCGCCACACCCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTc                                                                                                                       >  1:826326/1‑140 (MQ=255)              aGCGCCACACCCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTc                                                                                                                       >  1:22104/1‑140 (MQ=255)              aGCGCCACACCCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTc                                                                                                                       >  1:40953/1‑140 (MQ=255)                     cacCCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTctct                                                                                                                >  1:259622/1‑140 (MQ=255)                               agccagccCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCg                                                                                                      <  1:991262/140‑1 (MQ=255)                                gccagccCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCgg                                                                                                     <  2:251387/140‑1 (MQ=255)                                  cagccCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGAt                                                                                                   <  2:622547/140‑1 (MQ=255)                                  cagccCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGAt                                                                                                   >  1:685501/1‑140 (MQ=255)                                                  cGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGt                                                                                   <  2:685501/140‑1 (MQ=255)                                                       gACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGtt                                                                              <  2:62581/140‑1 (MQ=255)                                                       gACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGtt                                                                              <  2:259622/140‑1 (MQ=255)                                                       gACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGtt                                                                              <  2:147475/140‑1 (MQ=255)                                                          aTCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCTGTCGCATCCAGCGTCAGTTTGt                                                                           >  2:712300/1‑140 (MQ=255)                                                            cgcgCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTa                                                                         <  1:741196/140‑1 (MQ=255)                                                              cgcAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAAc                                                                       >  1:565975/1‑140 (MQ=255)                                                                 aGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACCTAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCaa                                                                    <  2:1008068/140‑1 (MQ=255)                                                                                  aTTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCCCTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCgaaga                                                   <  1:320717/140‑1 (MQ=255)                                                                                     ttGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACg                                                <  2:918858/140‑1 (MQ=255)                                                                                     ttGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACg                                                <  1:921127/140‑1 (MQ=255)                                                                                     ttGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACg                                                <  2:1066983/140‑1 (MQ=255)                                                                                       gAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGc                                              <  1:953107/140‑1 (MQ=255)                                                                                          gTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATg                                           <  1:771485/140‑1 (MQ=255)                                                                                          gTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATg                                           <  2:599811/140‑1 (MQ=255)                                                                                              cGCTGACCGGGACATAATGGCTTACCCCTTCTCCAGTTTAATCGCGAACTTCTTGAAGTCCGTCTCTTTCGAAAGAGGATCGGTCGCATCCAGCGTCAGTTTGTTATCCAACTGTGCGGCGTCGAAGAACGGCATGTacc                                       >  2:858834/1‑139 (MQ=255)                                                                                              cGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTaca                                       >  2:1050026/1‑140 (MQ=255)                                                                                              cGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTaca                                       <  1:49962/140‑1 (MQ=255)                                                                                                        gACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATg                                           <  1:663633/126‑1 (MQ=255)                                                                                                        gACATAATGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATg                                           >  2:663633/1‑126 (MQ=255)                                                                                                              aTGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCTGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCgg                       <  1:712300/140‑1 (MQ=255)                                                                                                              aTGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCgg                       <  2:793431/140‑1 (MQ=255)                                                                                                              aTGGCTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCgg                       <  1:1050026/140‑1 (MQ=255)                                                                                                                  cTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACgg                   >  2:285798/1‑140 (MQ=255)                                                                                                                  cTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACgg                   >  1:65080/1‑140 (MQ=255)                                                                                                                  cTTACACCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACgg                   >  2:956422/1‑140 (MQ=255)                                                                                                                       acCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCgg                       >  2:129744/1‑131 (MQ=255)                                                                                                                       acCTTCTCCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCgg                       <  1:129744/131‑1 (MQ=255)                                                                                                                           tctcCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCgg                       <  2:822087/127‑1 (MQ=255)                                                                                                                           tctcCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCgg                       >  1:822087/1‑127 (MQ=255)                                                                                                                           tctcCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACcg          >  1:777072/1‑140 (MQ=255)                                                                                                                            ctcCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACcgc         >  1:504683/1‑140 (MQ=255)                                                                                                                             tcCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACcgcg        >  1:323777/1‑140 (MQ=255)                                                                                                                             tcCAGTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACcgcg        <  1:956422/140‑1 (MQ=255)                                                                                                                                 gTTTGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACCGCGCGtt    >  2:353496/1‑140 (MQ=255)                                                                                                                                   ttGATCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACCGCGCGATTc  >  1:388363/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        CTGTTGTAACCCCAGCGCCACACCCACGGCAGCCAGCCCGCCTGCTGTGCGAACGACATCGCGCAGAAAGCGGCGGCGACCATTTTGAGGTTTCGCTGACCGGGACATAATGGCTTACACCTTCTCCAGTTTGACCGCGCACTTCTTGAAGTCCGTCTCTTTCGAGAGCGGATCGGTCGCATCCAGCGTCAGTTTGTTAACCAACTGTGCGGCGTCGAAGAACGGCATGTACACCAGGCCCTGTGGCGGACGGTTACGACCGCGCGTTTC  >  NC_000913/2300153‑2300422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑