Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,301,322 T→C T478A (ACC→GCC)  napA ← periplasmic nitrate reductase subunit NapA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,301,3220TC100.0% 151.2 / NA 42T478A (ACC→GCC) napAperiplasmic nitrate reductase subunit NapA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/20);  total (22/20)

CAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGCAGATATCACGGTGTTTCTCGTTGGTCACC  >  NC_000913/2301185‑2301458
                                                                                                                                         |                                                                                                                                        
cAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGccc                                                                                                                                        <  2:85164/140‑1 (MQ=255)
   gATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGt                                                                                                                                     <  1:260672/140‑1 (MQ=255)
   gATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGt                                                                                                                                     <  1:509920/140‑1 (MQ=255)
          tCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACAtt                                                                                                                              <  2:618562/140‑1 (MQ=255)
             gcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAg                                                                                                                           >  1:746013/1‑140 (MQ=255)
                               gTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAgcgc                                                                                                         >  1:1003529/1‑140 (MQ=255)
                                 cGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCAc                                                                                                       <  1:548014/140‑1 (MQ=255)
                                     tACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTc                                                                                                   <  1:659025/140‑1 (MQ=255)
                                      aCGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCt                                                                                                  >  2:244033/1‑140 (MQ=255)
                                      aCGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCt                                                                                                  >  2:138987/1‑140 (MQ=255)
                                          aTCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTtgtg                                                                                              >  2:77517/1‑140 (MQ=255)
                                              gagaCGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCAGTCTTTCAGCGCACGGTCTTGTGCCAc                                                                                          >  1:956432/1‑140 (MQ=255)
                                                    atgatgAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTg                                                                                    <  2:65334/140‑1 (MQ=255)
                                                             ttGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGAt                                                                           <  1:138987/140‑1 (MQ=255)
                                                                     aTCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCg                                                                   >  1:634305/1‑140 (MQ=255)
                                                                       cgcgCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGa                                                                 <  2:128272/140‑1 (MQ=255)
                                                                               cccGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGcc                                                         >  1:355850/1‑140 (MQ=255)
                                                                               cccGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGcc                                                         >  1:256426/1‑140 (MQ=255)
                                                                                      tACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCggg                                                  <  2:256426/140‑1 (MQ=255)
                                                                                       aCGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGa                                                 <  2:1003529/140‑1 (MQ=255)
                                                                                            ctTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTc                                            >  2:447832/1‑140 (MQ=255)
                                                                                                aTTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCAct                                        <  1:495482/140‑1 (MQ=255)
                                                                                                aTTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCAct                                        <  2:660441/140‑1 (MQ=255)
                                                                                                    aTGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCt                                                      >  1:126043/1‑122 (MQ=255)
                                                                                                    aTGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCt                                                      <  2:126043/122‑1 (MQ=255)
                                                                                                       ttCGGCCCGGCCTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTtctc                                 >  2:848893/1‑140 (MQ=255)
                                                                                                         cggcccggccTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGc                               >  1:57844/1‑140 (MQ=255)
                                                                                                         cggcccggccTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGc                               <  1:447832/140‑1 (MQ=255)
                                                                                                               ggccTGCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGCAGatat                         <  1:77517/140‑1 (MQ=255)
                                                                                                                    gCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGc                               >  1:73966/1‑129 (MQ=255)
                                                                                                                    gCATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGc                               <  2:73966/129‑1 (MQ=255)
                                                                                                                     cATGTTGTTGGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGCAGATATCACGgt                   >  2:817183/1‑140 (MQ=255)
                                                                                                                          tgttgGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGCAGATATCACGGTGTTtc              >  2:499137/1‑140 (MQ=255)
                                                                                                                           gttgGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGTAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGCAGATATCACGGTGTTtct             >  2:335261/1‑140 (MQ=255)
                                                                                                                             tgGTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGCAGATATCACGGTGTTTCTCg           <  2:746013/140‑1 (MQ=255)
                                                                                                                               gTACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGCAGATATCACGGTGTTTCTCGtt         >  1:100752/1‑140 (MQ=255)
                                                                                                                                tACACATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGCAGATATCACGGTGTTTCTCGTTg        >  2:443068/1‑140 (MQ=255)
                                                                                                                                 acacATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGCAGATATCACGGTGTTTCTCGTTgg       <  2:292932/140‑1 (MQ=255)
                                                                                                                                 acacATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGCAGATATCACGGTGTTTCTCGTTgg       >  2:318745/1‑140 (MQ=255)
                                                                                                                                 acacATGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGCAGATATCACGGTGTTTCTCGTTgg       >  1:412594/1‑140 (MQ=255)
                                                                                                                                  cacaTGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGCAGATATCACGGTGTTTCTCGTTGGt      >  1:625891/1‑140 (MQ=255)
                                                                                                                                      tGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGCAGATATCACGGTGTTTCTCg           <  1:766705/131‑1 (MQ=255)
                                                                                                                                      tGGCCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGCAGATATCACGGTGTTTCTCGTTGGTcacc  <  1:132557/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCAGTTTGCCGTCTTTCAGCGCACGGTCTTGTGCCACCGCGTGCAGACCGATTTTCGCCGGAATGGTGCCGCTCGGGATATTCCACTTCTTCTCGCAGATATCACGGTGTTTCTCGTTGGTCACC  >  NC_000913/2301185‑2301458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑