Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	191,783	T→C	intergenic (+75/‑72)	tsf → / → pyrH	protein chain elongation factor EF‑Ts/UMP kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	191,783	0	T	C	100.0%	154.2 / NA	42	intergenic (+75/‑72)	tsf/pyrH	protein chain elongation factor EF‑Ts/UMP kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/22);  total (20/22)

CATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAACGCATTCTGCTTAAGTTGAGTGGCGAAGCTCT  >  NC_000913/191648‑191916                                                                                                                                        |                                                                                                                                      cATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCgcgc                                                                                                                                   >  1:822476/1‑140 (MQ=255)   tCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTg                                                                                                                                 >  1:53837/1‑140 (MQ=255)    cGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGa                                                                                                                                >  2:191330/1‑140 (MQ=255)       gaAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGaaaa                                                                                                                             <  2:53837/140‑1 (MQ=255)        aaaGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAg                                                                                                                            <  2:481391/140‑1 (MQ=255)        aaaGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAg                                                                                                                            <  2:983607/140‑1 (MQ=255)          aGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCg                                                                                                                          <  1:914691/140‑1 (MQ=255)                   tgactTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAAt                                                                                                                 <  1:476936/140‑1 (MQ=255)                     actTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGt                                                                                                               >  1:358320/1‑140 (MQ=255)                     actTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGt                                                                                                               >  1:453313/1‑140 (MQ=255)                        ttGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGc                                                                                                            <  1:761847/140‑1 (MQ=255)                              cagAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTAt                                                                                                      <  1:113483/140‑1 (MQ=255)                                        tgcGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCAttt                                                                                            >  2:227891/1‑140 (MQ=255)                                               tCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGt                                                                                     >  2:198959/1‑140 (MQ=255)                                               tCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGt                                                                                     >  1:698522/1‑140 (MQ=255)                                                  aaGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGa                                                                                  <  1:165515/140‑1 (MQ=255)                                                       gTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGAc                                                                                 >  2:862301/1‑136 (MQ=255)                                                       gTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGAc                                                                                 <  1:862301/136‑1 (MQ=255)                                                                  cAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGtctc                                                                           <  2:558711/131‑1 (MQ=255)                                                                  cAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGtctc                                                                           >  1:558711/1‑131 (MQ=255)                                                                  cAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGgaaagaa                                                                  >  1:67758/1‑140 (MQ=255)                                                                  cAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGgaaagaa                                                                  >  2:313429/1‑140 (MQ=255)                                                                  cAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGgaaagaa                                                                  >  2:606011/1‑140 (MQ=255)                                                                             cgccTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTAcc                                                       <  1:95025/140‑1 (MQ=255)                                                                             cgccTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTAcc                                                       <  1:32752/140‑1 (MQ=255)                                                                                   aGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCa                                                 >  1:1000912/1‑140 (MQ=255)                                                                                         gcTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAAccc                                           <  2:1000912/140‑1 (MQ=255)                                                                                         gcTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAAccc                                           >  2:32754/1‑140 (MQ=255)                                                                                          cttcttTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCg                                          >  1:111731/1‑140 (MQ=255)                                                                                              tttttGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCta                                      <  2:217938/140‑1 (MQ=255)                                                                                                      cccATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAACGCa                              <  1:401186/140‑1 (MQ=255)                                                                                                       ccATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAACGCAt                             <  2:111731/140‑1 (MQ=255)                                                                                                       ccATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAACGCAt                             <  2:67758/140‑1 (MQ=255)                                                                                                       ccATCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAACGCAt                             <  2:698522/140‑1 (MQ=255)                                                                                                         aTCTTGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAACGCATTc                           <  1:736312/140‑1 (MQ=255)                                                                                                             tGTAAATTCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATATACGCATTCTGCt                       >  2:409085/1‑140 (MQ=255)                                                                                                                    tCAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAACGCATTCTGCTTAAGTTg                <  1:313429/140‑1 (MQ=255)                                                                                                                     cAGCTAACCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAACGCATTCTGCTTAAGTTGa               >  1:94641/1‑140 (MQ=255)                                                                                                                           aCCCTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAACGCATTCTGCTTAAGTTGAGTGGCg         <  2:820484/140‑1 (MQ=255)                                                                                                                              cTTGTGGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAACGCATTCTGCTTAAGTTGAGTGGCGAAg      <  2:822476/140‑1 (MQ=255)                                                                                                                                tgtgGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAACGCATTCTGCTTAAGTTGAGTGGCGAAGct    >  1:315001/1‑140 (MQ=255)                                                                                                                                  tgGGGCCGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAACGCATTCTGCTTAAGTTGAGTGGCGAAActct  >  1:346764/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCAGTCTTAATTATCAAAAAGGAGCCGCCTGAGGGCGGCTTCTTTTTGTGCCCATCTTGTAAATTCAGCTAACCCTTGTGGGGCTGCGCTGAAAAGCGACGTACAATGTCGCTAGTATTAATTCATTTCAATCGTTGACAGTCTCAGGAAAGAAACATGGCTACCAATGCAAAACCCGTCTATAAACGCATTCTGCTTAAGTTGAGTGGCGAAGCTCT  >  NC_000913/191648‑191916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑