Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,342,036	A→G	F712L (TTC→CTC)	yfaL ←	putative autotransporter adhesin YfaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,342,036	0	A	G	100.0%	259.0 / NA	70	F712L (TTC→CTC)	yfaL	putative autotransporter adhesin YfaL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/39);  total (31/39)

CGTTAAATAGTGAGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAACGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAGCTCCAGTGTACCAGCCC  >  NC_000913/2341901‑2342170                                                                                                                                        |                                                                                                                                       cGTTAAATAGTGAGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGtt                                                                                                                                    <  1:129150/140‑1 (MQ=255) cGTTAAATAGTGAGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGtt                                                                                                                                    <  2:532471/140‑1 (MQ=255)   ttAAATAGTGAGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGc                                                                                                                                  >  1:1054007/1‑140 (MQ=255)      aaTAGTGAGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGcccc                                                                                                                               <  1:257469/140‑1 (MQ=255)      aaTAGTGAGGCATTAAGGGCGACGGAAGTATCCTACCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCG‑‑AGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCAt                                                                                                                             <  2:823325/140‑1 (MQ=255)        tAGTGAGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCAt                                                                                                                             <  2:955202/140‑1 (MQ=255)        tAGTGAGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCAt                                                                                                                             <  2:1054007/140‑1 (MQ=255)        tAGTGAGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCAt                                                                                                                             <  2:219380/140‑1 (MQ=255)        tAGTGAGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCACTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCAt                                                                                                                             <  1:592350/140‑1 (MQ=255)           tGAGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCAc                                                                                                                          <  1:591457/140‑1 (MQ=255)           tGAGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCAc                                                                                                                          >  2:268562/1‑140 (MQ=255)           tGAGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCAc                                                                                                                          >  2:39648/1‑140 (MQ=255)           tGAGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCAc                                                                                                                          >  2:66260/1‑140 (MQ=255)           tGAGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCAc                                                                                                                          >  1:321745/1‑140 (MQ=255)             aGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCa                                                                                                                        <  1:66260/140‑1 (MQ=255)             aGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCa                                                                                                                        >  1:598603/1‑140 (MQ=255)             aGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCa                                                                                                                        >  1:266490/1‑140 (MQ=255)               gCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAAc                                                                                                                      <  1:775388/140‑1 (MQ=255)                cATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAAcc                                                                                                                     >  1:685049/1‑140 (MQ=255)                    aaGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCcaca                                                                                                                 <  1:680358/140‑1 (MQ=255)                     aGGGCGACGTAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAg                                                                                                                >  1:1032358/1‑140 (MQ=255)                     aGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAg                                                                                                                >  1:1047719/1‑140 (MQ=255)                        gCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGa                                                                                                             <  1:303781/140‑1 (MQ=255)                        gCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGa                                                                                                             <  2:281430/140‑1 (MQ=255)                          gACGTAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACg                                                                                                           <  2:1032358/140‑1 (MQ=255)                          gACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACg                                                                                                           <  2:321745/140‑1 (MQ=255)                          gACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACg                                                                                                           <  1:594871/140‑1 (MQ=255)                                aGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCAt                                                                                                     <  1:268562/140‑1 (MQ=255)                                     ccTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATaagga                                                                                                <  2:957008/140‑1 (MQ=255)                                        gCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATaaggaagg                                                                                             <  2:598603/140‑1 (MQ=255)                                          ccGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGat                                                                                           <  1:39648/140‑1 (MQ=255)                                          ccGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGat                                                                                           <  2:723405/140‑1 (MQ=255)                                           cGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGata                                                                                          <  2:266490/140‑1 (MQ=255)                                            ggTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGatat                                                                                         >  2:254434/1‑140 (MQ=255)                                            ggTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGatat                                                                                         >  2:938518/1‑140 (MQ=255)                                              tCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCa                                                                                       >  1:102201/1‑140 (MQ=255)                                                  gcgcAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATaaggaagg                                                                                             <  1:834104/130‑1 (MQ=255)                                                  gcgcAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATaaggaagg                                                                                             >  2:834104/1‑130 (MQ=255)                                                      aaaaCCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATc                                                                               <  1:938518/140‑1 (MQ=255)                                                        aaCCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCAc                                                                             >  2:1010338/1‑140 (MQ=255)                                                            gTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCttt                                                                         <  1:458753/140‑1 (MQ=255)                                                                     cTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGcc                                                                <  2:506650/140‑1 (MQ=255)                                                                         tGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCcttct                                                            <  1:1010338/140‑1 (MQ=255)                                                                            cGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCg                                                         >  1:364642/1‑140 (MQ=255)                                                                             gATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGa                                                        >  2:968846/1‑140 (MQ=255)                                                                              aTAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGGCGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGac                                                       >  1:295752/1‑140 (MQ=255)                                                                                          gAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGa                                           <  1:968846/140‑1 (MQ=255)                                                                                          gAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGa                                           <  1:57249/140‑1 (MQ=255)                                                                                          gAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGa                                           <  1:386219/140‑1 (MQ=255)                                                                                              tAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGc                                       >  2:437164/1‑140 (MQ=255)                                                                                                gCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGt                                     <  1:514712/140‑1 (MQ=255)                                                                                                             gAATATCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCg                        <  2:458077/140‑1 (MQ=255)                                                                                                               atatCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGt                      <  2:685049/140‑1 (MQ=255)                                                                                                                 atCCTGATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCa                    >  1:555672/1‑140 (MQ=255)                                                                                                                      gATCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAGCTcc               <  1:904728/140‑1 (MQ=255)                                                                                                                       aTCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAGCTCCa              <  1:391307/140‑1 (MQ=255)                                                                                                                        tCCGCGCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAGCTCCAg             >  1:166128/1‑140 (MQ=255)                                                                                                                          cgcgCCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAGCTCCAgtg           >  1:657166/1‑140 (MQ=255)                                                                                                                            cgcCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAg                   <  2:249034/130‑1 (MQ=255)                                                                                                                            cgcCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAg                   >  1:249034/1‑130 (MQ=255)                                                                                                                            cgcCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAGCTc                <  2:721277/133‑1 (MQ=255)                                                                                                                            cgcCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAGCTc                >  1:721277/1‑133 (MQ=255)                                                                                                                            cgcCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAGCTCCa              >  2:159327/1‑135 (MQ=255)                                                                                                                            cgcCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAGCTCCa              <  1:159327/135‑1 (MQ=255)                                                                                                                            cgcCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAGCTCCAGTGTa         >  1:495646/1‑140 (MQ=255)                                                                                                                            cgcCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAGCTCCAGTGTa         >  2:527585/1‑140 (MQ=255)                                                                                                                            cgcCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAGCTCCAGTGTa         >  2:706451/1‑140 (MQ=255)                                                                                                                            cgcCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAGCTCCAGTGTa         >  2:508349/1‑140 (MQ=255)                                                                                                                            cgcCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAGCTCCAGTGTa         >  1:504630/1‑140 (MQ=255)                                                                                                                            cgcCAGTAACGAGCGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAGCTCCAGTGTa         >  2:242754/1‑140 (MQ=255)                                                                                                                                   aacgagcgTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAGCTCCAGTGTACCAGccc  <  2:102201/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       CGTTAAATAGTGAGGCATTAAGGGCGACGGAAGTATCCTGCCCGGTCAGGCGCAAAACCGTACCATCGCTGATGTCGATAGTGCCGCTGGAAATAGCATCAATTGACTGAATATCCTGATCCGCGCCAGTAACGAACGTTGCCCCATCACCAACCCACAGTGACGAAGCATAAGGAAGGATATCAGCAACATCACCTTTCAGGGTGCCTTCTTCGACACGTACCGCCGACTGCGTTGTACCGCTGGCGGTCAGCTCCAGTGTACCAGCCC  >  NC_000913/2341901‑2342170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑