Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,367,339	A→G	H90R (CAC→CGC)	arnC →	undecaprenyl‑phosphate 4‑deoxy‑4‑formamido‑L‑arabinose transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,367,339	0	A	G	100.0%	80.1 / NA	23	H90R (CAC→CGC)	arnC	undecaprenyl‑phosphate 4‑deoxy‑4‑formamido‑L‑arabinose transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/23);  total (0/23)

TGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑AATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGT  >  NC_000913/2367208‑2367450                                                                                                                                    |                                                                                                                 tgatgaCGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTAGTCGCGGTACTg                                                                                                          >  2:872435/1‑140 (MQ=255) tgatgaCGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTg                                                                                                          <  2:332030/140‑1 (MQ=255)     gaCGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGa                                                                                                      <  2:875242/140‑1 (MQ=255)       cGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACt                                                                                                    <  1:37146/140‑1 (MQ=255)         gCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTTAGTCGCGTTTCTGGGGGCttt                                                                                                  >  2:51731/1‑139 (MQ=255)               gCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAattatt                                                                                            <  1:220216/140‑1 (MQ=255)               gCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATCGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAattatt                                                                                            <  1:313712/140‑1 (MQ=255)                           cgcATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTTATCGCGGTACTGGGGGCCTAAATATTTTCCTTTATggc                                                                                >  2:869454/1‑138 (MQ=255)                           cgcATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAattat                                                                                             >  1:394308/1‑127 (MQ=255)                           cgcATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAattat                                                                                             <  2:394308/127‑1 (MQ=255)                             cATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAATTATTACCCTTGATGCCGa                                                                              <  1:22092/140‑1 (MQ=255)                                atGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGGTTAGGTGCGTTTCTTGCGGCTTAATTTTTTTCCTTTATTCCGAtct                                                                           >  2:476435/1‑140 (MQ=255)                                      gTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTAGTCCCGGTTCTGGCGGCCTAAATATTAACCCTTTTGCCGGTCTcccgca                                                                     >  2:22092/1‑136 (MQ=255)                                      gTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑aa                                                                    <  1:869454/140‑1 (MQ=255)                                        cGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTCAGTCGCGCTACTGGCGCCTTAATTATTATCCCTTATGCCCATCTCCCG‑aaac                                                                  >  2:772128/1‑138 (MQ=255)                                        cGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTCAATCGCGGTTCTGGGGGCGTAATTATTTTCCTTTTTGCCGGTCTCCCG‑aaac                                                                  >  1:1026960/1‑138 (MQ=255)                                           aGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑AATCCGc                                                               <  1:476435/140‑1 (MQ=255)                                            gCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑AATCCGcc                                                              <  2:692820/140‑1 (MQ=255)                                              agcacaAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑AATCCGCCag                                                            <  1:1054914/138‑1 (MQ=255)                                                     gCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑AATCCGCCAGAAGAAAt                                                     <  2:434369/140‑1 (MQ=255)                                                      cGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGGTTTAGTCGCGTTACTGGCGGCTTAATTTTTTTTCTTTATTCCGGTCTCCCC‑AATCCCCCCGAAGaaaaa                                                    >  2:473111/1‑138 (MQ=255)                                                            aCAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑AATCCGCCAGAAGAAATCCCCCGc                                              <  1:51731/140‑1 (MQ=255)                                                                            tATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑AATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCg                              <  1:810243/140‑1 (MQ=255)                                                                                  gCTTAACCGCAATTACGGGTAACATTCAGCGATTATGGCGGGGGTTAATCGCGTTTCTGGGGGCCTTATTTTTTTCCTTTATGCCGGTCTCCCG‑AAACCCCCCGAAAAAAAACCCCCCCCGGTGGGGAAAACCCATGAAg                        >  2:168346/1‑140 (MQ=255)                                                                                      aaCCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑AATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTa                    <  1:872435/140‑1 (MQ=255)                                                                                      aaCCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑AATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTa                    <  1:747721/140‑1 (MQ=255)                                                                                        ccGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑AATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacg                  <  2:186110/140‑1 (MQ=255)                                                                                          gCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑AATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                <  1:873270/140‑1 (MQ=255)                                                                                          gCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑AATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                <  1:527954/140‑1 (MQ=255)                                                                                          gCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑AATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                <  1:478146/140‑1 (MQ=255)                                                                                          gCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑AATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                <  1:473111/140‑1 (MQ=255)                                                                                          gCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑AATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTacgac                <  1:443407/140‑1 (MQ=255)                                                                                           cAATTACGGGCAACATTCAGCGATTATGGCGGGGTTCAGTCGCGGTACTGGCGGCTTAATTATTATCCTTTGTGTCGCTCTCCCGAAACCCCCCCAAAAAAAACTCCCCCCCGGGGGAAAAAG‑CGCTGAAGGGTaagacg               >  2:747721/1‑140 (MQ=255)                                                                                            aaTTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCGCGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑AATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGt              <  2:1042522/140‑1 (MQ=255)                                                                                                        cATTCAGCGATTATGGCGGGGTTTAGTCGCGGTTCTGGCGGCCTAATTATTATCCCTTATGCCGGTCTCCCG‑AAATCCCCCCAAGAAAAACCCCCCCCCGGGGCAAAAAACCATGAAAGGTACGACGGGGGAGGGGCTgg  >  2:426981/1‑139 (MQ=255)                                                                                                        cATTCAGCGATTATGGCGGGATGTTGACGCGGTAGTGTCGGCCTTATTATTATCCCTTATGCCGATCTCCTG‑AATCCCCCCGAAGAAAAACCCCCCCCCGTGGGGAAAAACGCTGAAGGTTACGACGTGGGAGGGGCTGt  >  2:984097/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                 TGATGACGGCAGTAGCGATAATTCCGCGCATATGCTGGTCGAAGCCTCACAAGCGGAGAACAGCCATATTGTGTCTATTTTGCTTAACCGCAATTACGGGCAACATTCAGCGATTATGGCGGGATTCAGTCACGTTACTGGCGACTTAATTATTACCCTTGATGCCGATCTCCAG‑AATCCGCCAGAAGAAATCCCCCGCCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGT  >  NC_000913/2367208‑2367450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑