Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,446,579	T→C	T299A (ACC→GCC)	aroC ←	chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,446,579	0	T	C	100.0%	162.3 / NA	45	T299A (ACC→GCC)	aroC	chorismate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/18);  total (27/18)

CAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCG  >  NC_000913/2446442‑2446711                                                                                                                                          |                                                                                                                                     cAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCaa                                                                                                                                    >  1:721911/1‑140 (MQ=255)    gTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGc                                                                                                                                 >  2:273386/1‑140 (MQ=255)    gTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGc                                                                                                                                 >  2:536704/1‑140 (MQ=255)     tGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCt                                                                                                                                >  1:46021/1‑140 (MQ=255)       aTCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTgg                                                                                                                              >  2:155936/1‑140 (MQ=255)            ttAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTc                                                                                                                         >  2:935794/1‑140 (MQ=255)            ttAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTc                                                                                                                         >  2:423911/1‑140 (MQ=255)              aaaaCGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTcag                                                                                                                       >  2:760162/1‑138 (MQ=255)              aaaaCGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCgg                                                                                                                       >  2:375838/1‑140 (MQ=255)                    aTCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAg                                                                                                                 <  1:375838/140‑1 (MQ=255)                    aTCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAg                                                                                                                 >  1:533144/1‑140 (MQ=255)                    aTCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAg                                                                                                                 <  1:536704/140‑1 (MQ=255)                    aTCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAg                                                                                                                 >  1:769391/1‑140 (MQ=255)                    aTCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTCGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAg                                                                                                                 >  1:147878/1‑140 (MQ=255)                     tCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAgc                                                                                                                <  2:533144/140‑1 (MQ=255)                      cGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAg                                                                                                                            >  2:884089/1‑127 (MQ=255)                      cGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAg                                                                                                                            <  1:884089/127‑1 (MQ=255)                      cGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAgcg                                                                                                               <  2:721911/140‑1 (MQ=255)                          aGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCat                                                                                                           <  2:1011070/140‑1 (MQ=255)                           gCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCata                                                                                                          <  2:869116/140‑1 (MQ=255)                                   tCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAAt                                                                                                  <  1:273386/140‑1 (MQ=255)                                              cACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTgccgcc                                                                                       <  2:1004761/140‑1 (MQ=255)                                                cTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCg                                                                                     <  2:46021/140‑1 (MQ=255)                                                   cgcgGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCgctg                                                                                  >  1:800459/1‑140 (MQ=255)                                                      ggATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGctgctg                                                                               <  1:835807/140‑1 (MQ=255)                                                                  ggggATCGTGACGGCTTTGGGTGATCATCTCAACTCCTCCGCCAAAGCGGTTAAGGGTCCGCCCCGGCACGGCAATGCTGGAGGTCGGTTCCAGCGCCATATGGGCAATGATTTGCTGCCCGCTTCTGATACCGCCgaga                                                                   <  2:106384/139‑1 (MQ=255)                                                                  aGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCgaga                                                                   >  1:111135/1‑140 (MQ=255)                                                                   gggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGaa                                                                  <  1:935794/140‑1 (MQ=255)                                                                    ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                 >  1:966749/1‑140 (MQ=255)                                                                    ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                 >  2:562359/1‑140 (MQ=255)                                                                         gTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATgccgc                                                            <  1:155936/140‑1 (MQ=255)                                                                            aCGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGccgcccg                                                         >  2:47970/1‑140 (MQ=255)                                                                            aCGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGccgcccg                                                         >  1:1022979/1‑140 (MQ=255)                                                                            aCGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGccgcccg                                                         >  2:1034563/1‑140 (MQ=255)                                                                              ggCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCa                                                       >  1:738039/1‑140 (MQ=255)                                                                                 cTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATgg                                                    <  1:423911/140‑1 (MQ=255)                                                                                 cTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATgg                                                    <  1:388562/140‑1 (MQ=255)                                                                                 cTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATgg                                                    <  1:562359/140‑1 (MQ=255)                                                                                         atcatcTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTg                                            >  2:541248/1‑140 (MQ=255)                                                                                              ctcAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAAc                                       >  2:341662/1‑140 (MQ=255)                                                                                                                  ggTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTGGGTTTCAGCCCAATACGGGCATTGATTTGCTCCCCGGTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCCTTGGTGATTTCATcg                   <  2:800459/140‑1 (MQ=255)                                                                                                                            aCGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGc         >  2:428869/1‑140 (MQ=255)                                                                                                                             cGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCt        >  2:602228/1‑140 (MQ=255)                                                                                                                                cccGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGcc     >  2:1058478/1‑140 (MQ=255)                                                                                                                                 ccGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcg    <  2:708263/140‑1 (MQ=255)                                                                                                                                  cggcacggcaATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcgc   <  1:541248/140‑1 (MQ=255)                                                                                                                                  cggcacggcaATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcgc   >  2:900814/1‑140 (MQ=255)                                                                                                                                  cggcacggcaATGCTGGAGGTCAGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcgc   <  1:760162/140‑1 (MQ=255)                                                                                                                                   ggCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcgcg  >  1:334205/1‑140 (MQ=255)                                                                                                                                   ggCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcgcg  <  1:341662/140‑1 (MQ=255)                                                                                                                                   ggCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcgcg  <  2:334205/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     CAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCG  >  NC_000913/2446442‑2446711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑