Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,838,610	T→C	N219D (AAC→GAC)	ascG ←	DNA‑binding transcriptional repressor AscG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,838,610	0	T	C	100.0%	156.0 / NA	44	N219D (AAC→GAC)	ascG	DNA‑binding transcriptional repressor AscG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/22);  total (22/22)

GCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATC  >  NC_000913/2838479‑2838743                                                                                                                                    |                                                                                                                                      gcgcTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATaa                                                                                                                               <  2:501484/140‑1 (MQ=255)         gtAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCa                                                                                                                       <  1:607585/140‑1 (MQ=255)                tttCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAgcg                                                                                                                <  2:503976/140‑1 (MQ=255)                 ttCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAgcgc                                                                                                               >  2:234400/1‑140 (MQ=255)                 ttCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAgcgc                                                                                                               >  1:995168/1‑140 (MQ=255)                  tCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCa                                                                                                              >  2:673055/1‑140 (MQ=255)                     tCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATa                                                                                                           <  1:27576/140‑1 (MQ=255)                      cGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATAc                                                                                                          <  1:872537/140‑1 (MQ=255)                       gCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATAcc                                                                                                         >  1:929328/1‑140 (MQ=255)                        cACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCa                                                                                                        >  2:704770/1‑140 (MQ=255)                            tATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCAtgct                                                                                                    >  2:200020/1‑140 (MQ=255)                              tCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAgcg                                                                                                                >  1:116914/1‑126 (MQ=255)                              tCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAgcg                                                                                                                <  2:116914/126‑1 (MQ=255)                                     atCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAgcg                                                                                           <  2:1062514/140‑1 (MQ=255)                                     atCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAgcg                                                                                           >  1:564451/1‑140 (MQ=255)                                     atCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAgcg                                                                                           >  2:615697/1‑140 (MQ=255)                                        gtcgtTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGt                                                                                        >  1:615214/1‑140 (MQ=255)                                         tcgtTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGcgcg                                                                                         >  2:126515/1‑138 (MQ=255)                                         tcgtTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGcgcg                                                                                         <  1:126515/138‑1 (MQ=255)                                         tcgtTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTc                                                                                       >  2:900004/1‑140 (MQ=255)                                            ttACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCttt                                                                                    >  2:849536/1‑140 (MQ=255)                                            ttACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCttt                                                                                    >  2:694587/1‑140 (MQ=255)                                               cTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTtata                                                                                 <  2:491224/140‑1 (MQ=255)                                                       tAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTACTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCaa                                                                         >  2:242854/1‑140 (MQ=255)                                                            cgcTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGt                                                                    >  1:98161/1‑140 (MQ=255)                                                            cgcTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGt                                                                    >  1:363168/1‑140 (MQ=255)                                                                aaaTTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCaa                                                                <  2:860170/140‑1 (MQ=255)                                                                  aTTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTNTATACCCGGCAAGACGTTCAATg                                                              <  2:995168/140‑1 (MQ=255)                                                                      aGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCtgg                                                          <  1:976647/140‑1 (MQ=255)                                                                       gCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCtggt                                                         >  2:847844/1‑140 (MQ=255)                                                                        ccccACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCtggtg                                                        <  1:626414/140‑1 (MQ=255)                                                                             cGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGa                                                   <  1:704770/140‑1 (MQ=255)                                                                                                    ttCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAg                            <  1:847844/140‑1 (MQ=255)                                                                                                     tCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGcc                                       >  1:697344/1‑128 (MQ=255)                                                                                                     tCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGcc                                       <  2:697344/128‑1 (MQ=255)                                                                                                     tCGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGc                           >  1:347129/1‑140 (MQ=255)                                                                                                      cGGCCCCGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCa                          <  1:673055/140‑1 (MQ=255)                                                                                                            cGCTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTGAGGAAAGCAATCTcc                    >  1:409100/1‑140 (MQ=255)                                                                                                              cTGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTg                  >  1:323935/1‑140 (MQ=255)                                                                                                               tGGCAGGCGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGa                 <  1:615697/140‑1 (MQ=255)                                                                                                                      cGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCgg          <  2:615214/140‑1 (MQ=255)                                                                                                                      cGTCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCgg          <  2:436872/140‑1 (MQ=255)                                                                                                                        tCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCg        <  1:242854/140‑1 (MQ=255)                                                                                                                        tCCATTTACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCg        <  2:347129/140‑1 (MQ=255)                                                                                                                              tACCGTCAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATc  <  1:326512/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      GCGCTCGTGTAACGCTTTCATCGCACCTATCGCCATATCGTCGTTACTGGCAACTAACGCGCTAAATTTAGCCCCACGTTCGAGCAACATTTCTACCCCTTCGGCCCCGCTGGCAGGCGTCCATTTACCGTTAGCGATAAGTTTTTCATTGAGCGCAATACCATGCTGCGCCAGCGCGTCTTTATACCCGGCAAGACGTTCAATGCTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAGCAATCTCCTGATGCCCGGCGTTTATC  >  NC_000913/2838479‑2838743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑