Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,912,216	T→C	N479S (AAC→AGC)	relA ←	GDP/GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,912,216	0	T	C	100.0%	166.1 / NA	47	N479S (AAC→AGC)	relA	GDP/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/25);  total (22/25)

TTTCAGGCTGATCCCCAGATGTTCCAGCTCGTCGTCAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGTTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGA  >  NC_000913/2912080‑2912348                                                                                                                                         |                                                                                                                                     tttCAGGCTGATCCCCAGATGTTCCAGCTCGTCGTCAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTg                                                                                                                                   >  2:9152/1‑140 (MQ=255)        cTGATCCCCAGATGTTCCAGCTCGTCGTCAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTaa                                                                                                                            <  2:1008929/140‑1 (MQ=255)           aTCCCCAGATGTTCCAGCTCGTCGTCAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCa                                                                                                                         >  1:909679/1‑140 (MQ=255)           aTCCCCAGATGTTCCAGCTCGTCGTCAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCa                                                                                                                         >  1:685679/1‑140 (MQ=255)            tCCCCAGATGTTCCAGCTCGTCGTCAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAg                                                                                                                        >  2:221939/1‑140 (MQ=255)               ccAGATGTTCCAGCTCGTCGTCAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCa                                                                                                                     <  2:828802/140‑1 (MQ=255)                   aTGTTCCAGCTCGTCGTCAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGc                                                                                                                 <  1:155306/140‑1 (MQ=255)                     gTTCCAGCTCGTCGTCAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGc                                                                                                                 >  1:139407/1‑138 (MQ=255)                     gTTCCAGCTCGTCGTCAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGc                                                                                                                 <  2:139407/138‑1 (MQ=255)                     gTTCCAGCTCGTCGTCAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTg                                                                                                               >  2:606804/1‑140 (MQ=255)                      ttCCAGCTCGTCGTCAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTgg                                                                                                              >  1:733865/1‑140 (MQ=255)                       tCCAGCTCGTCGTCAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTggg                                                                                                             >  1:153834/1‑140 (MQ=255)                        ccAGCTCGTCGTCAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAAc                                                                                                                           <  2:970511/125‑1 (MQ=255)                        ccAGCTCGTCGTCAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAAc                                                                                                                           >  1:970511/1‑125 (MQ=255)                        ccAGCTCGTCGTCAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTgggg                                                                                                            >  1:591977/1‑140 (MQ=255)                                tcgtcAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGtt                                                                                                          <  1:172725/134‑1 (MQ=255)                                tcgtcAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGtt                                                                                                          >  2:172725/1‑134 (MQ=255)                                 cgtcAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGAGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGt                                                                                                   >  2:1027334/1‑140 (MQ=255)                                    cAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTc                                                                                                <  1:525042/140‑1 (MQ=255)                                         aTTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGt                                                                                           <  2:153834/140‑1 (MQ=255)                                             gccgccCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATa                                                                                       >  2:698098/1‑140 (MQ=255)                                                 cccAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAAttt                                                                                   <  2:909679/140‑1 (MQ=255)                                                          aaTGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGt                                                                          <  2:179614/140‑1 (MQ=255)                                                             gTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGc                                                                       <  1:82394/140‑1 (MQ=255)                                                             gTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGc                                                                       <  2:733865/140‑1 (MQ=255)                                                             gTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGc                                                                       <  2:1023916/140‑1 (MQ=255)                                                                       cGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAg                                                             <  1:1070309/140‑1 (MQ=255)                                                                             tGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCa                                                              >  2:289051/1‑133 (MQ=255)                                                                             tGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCa                                                              <  1:289051/133‑1 (MQ=255)                                                                              gTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAg                                                      <  1:698098/140‑1 (MQ=255)                                                                              gTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAg                                                      <  1:606804/140‑1 (MQ=255)                                                                                   cGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGaa                                                 <  1:221939/140‑1 (MQ=255)                                                                                    ggAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAAc                                                >  2:695049/1‑140 (MQ=255)                                                                                    ggAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAAc                                                >  1:788266/1‑140 (MQ=255)                                                                                     gAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACg                                               <  1:561067/140‑1 (MQ=255)                                                                                      aaCCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACgg                                              >  2:725951/1‑140 (MQ=255)                                                                                           ggCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACaa                                         >  2:379802/1‑140 (MQ=255)                                                                                               tGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATgcg                                     <  2:788266/140‑1 (MQ=255)                                                                                                gAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATgcgc                                    >  2:826537/1‑140 (MQ=255)                                                                                                                acagCTGGTTGTGATGTAACCGAGGCTTGGATTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGccc                    <  2:662484/137‑1 (MQ=255)                                                                                                                        ttGTGACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAg            <  1:9152/140‑1 (MQ=255)                                                                                                                           tgACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCgg         <  2:591977/140‑1 (MQ=255)                                                                                                                            gACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCgg         <  2:355941/139‑1 (MQ=255)                                                                                                                            gACGTAACCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCgg         >  1:355941/1‑139 (MQ=255)                                                                                                                                 aaCCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCg   >  1:855471/1‑140 (MQ=255)                                                                                                                                  aCCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCTCAATGCGCCCGCCAATTTTTGCCCCGATGCAGCg          <  2:446047/132‑1 (MQ=255)                                                                                                                                  aCCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCTCAATGCGCCCGCCAATTTTTGCCCCGATGCAGCg          >  1:446047/1‑132 (MQ=255)                                                                                                                                  aCCGAGGCTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGa  <  1:695049/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TTTCAGGCTGATCCCCAGATGTTCCAGCTCGTCGTCAAGGATTTGCCGCCCAGCCAGAATGTTTTTGTCACGGTCCTGTTTACGGAACCAGGCGTGAATTTTCGAACGCCCACGGCTGGTTGTGACGTAACCGAGGTTTGGGTTTAACCAGTCACGGCTGGGGTTCGGCTGTTTCTGGGTGATAATTTCAATCTGGTCGCCCATCTGCAGCTGGTAGGTGAACGGCACAATGCGCCCGCCAATTTTTGCCCCGATGCAGCGGTGTCCGA  >  NC_000913/2912080‑2912348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑