Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,016,853	T→C	V265A (GTT→GCT)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,016,853	0	T	C	100.0%	262.8 / NA	72	V265A (GTT→GCT)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/30);  total (42/30)

GTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCGG  >  NC_000913/3016717‑3016988                                                                                                                                         |                                                                                                                                        gTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCg                                                                                                                                      >  1:869993/1‑140 (MQ=255) gTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGAATGAAATCGAAGGCATTTGCCGCTACATGCTTGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCg                                                                                                                                      >  2:174522/1‑140 (MQ=255)  tGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGt                                                                                                                                     <  1:382272/140‑1 (MQ=255)    cATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTga                                                                                                                                   >  2:655485/1‑140 (MQ=255)      tGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTgaga                                                                                                                                 >  2:369110/1‑140 (MQ=255)       ggCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGAt                                                                                                                                >  1:214383/1‑140 (MQ=255)       ggCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGAt                                                                                                                                >  2:242065/1‑140 (MQ=255)        gCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATc                                                                                                                               <  2:869993/140‑1 (MQ=255)           tCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGt                                                                                                                                     >  1:412439/1‑131 (MQ=255)           tCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGt                                                                                                                                     <  2:412439/131‑1 (MQ=255)            cACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCg                                                                                                                           >  1:449591/1‑140 (MQ=255)                       ccaTGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGt                                                                                                                >  1:217766/1‑140 (MQ=255)                       ccaTGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGt                                                                                                                >  2:104812/1‑140 (MQ=255)                       ccaTGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGt                                                                                                                <  1:688164/140‑1 (MQ=255)                          tGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTc                                                                                                             <  2:934782/140‑1 (MQ=255)                           gCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCg                                                                                                            >  2:434801/1‑140 (MQ=255)                            cACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAGCACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGt                                                                                                                >  2:738060/1‑135 (MQ=255)                            cACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGt                                                                                                                <  1:738060/135‑1 (MQ=255)                            cACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCgg                                                                                                           >  1:47772/1‑140 (MQ=255)                            cACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCgg                                                                                                           >  1:430304/1‑140 (MQ=255)                             aCGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGt                                                                                                          >  2:346059/1‑140 (MQ=255)                               ggCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTa                                                                                                        >  1:457783/1‑140 (MQ=255)                                 cTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACa                                                                                                      >  2:618844/1‑140 (MQ=255)                                 cTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACa                                                                                                      <  1:434801/140‑1 (MQ=255)                                 cTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTACTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACa                                                                                                      <  2:686670/140‑1 (MQ=255)                                  tGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACAt                                                                                                     <  2:169538/140‑1 (MQ=255)                                   gTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATa                                                                                                    <  1:346059/140‑1 (MQ=255)                                    tcctccGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACa                                                                                                      >  2:316861/1‑137 (MQ=255)                                    tcctccGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACa                                                                                                      <  1:316861/137‑1 (MQ=255)                                    tcctccGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAg                                                                                                   >  1:648116/1‑140 (MQ=255)                                     cctccGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAgg                                                                                                  <  1:368167/140‑1 (MQ=255)                                     cctccGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAgg                                                                                                  <  2:648116/140‑1 (MQ=255)                                         cGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTa                                                                                              >  2:598266/1‑140 (MQ=255)                                           cATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTaaa                                                                                            >  1:108790/1‑140 (MQ=255)                                             tGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAaag                                                                                          <  2:217766/140‑1 (MQ=255)                                               aaaTCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAaagaa                                                                                        <  1:190145/140‑1 (MQ=255)                                               aaaTCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAaagaa                                                                                        <  1:722665/140‑1 (MQ=255)                                                 aTCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAagaaga                                                                                      >  1:601316/1‑140 (MQ=255)                                                           tttGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGAt                                                                            >  1:339973/1‑140 (MQ=255)                                                              gCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCAc                                                                         <  1:104812/140‑1 (MQ=255)                                                                       tGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAg                                                                >  2:704730/1‑140 (MQ=255)                                                                         cTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCt                                                              <  2:457783/140‑1 (MQ=255)                                                                               gaaAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCa                                                        >  1:930367/1‑140 (MQ=255)                                                                               gaaAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCa                                                        <  2:601316/140‑1 (MQ=255)                                                                               gaaAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCa                                                        <  1:655485/140‑1 (MQ=255)                                                                                 aaaaGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAg                                                      <  1:618844/140‑1 (MQ=255)                                                                                      ggCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTg                                                 >  2:483299/1‑140 (MQ=255)                                                                                       gCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTgg                                                <  2:430304/140‑1 (MQ=255)                                                                                       gCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTgg                                                >  2:60734/1‑140 (MQ=255)                                                                                          cAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGaaa                                             >  1:529416/1‑140 (MQ=255)                                                                                           aaCACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGc                                                     >  2:856052/1‑131 (MQ=255)                                                                                           aaCACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGc                                                     <  1:856052/131‑1 (MQ=255)                                                                                           aaCACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAAt                                            >  1:385230/1‑140 (MQ=255)                                                                                              acCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCt                                         <  2:385230/140‑1 (MQ=255)                                                                                                cTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTgg                                       <  2:131505/140‑1 (MQ=255)                                                                                                  ttGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGaa                                     >  1:593402/1‑140 (MQ=255)                                                                                                  ttGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGaa                                     >  1:865615/1‑140 (MQ=255)                                                                                                  ttGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGaa                                     <  1:711267/140‑1 (MQ=255)                                                                                                  ttGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGaa                                     <  2:214383/140‑1 (MQ=255)                                                                                                   tgtgAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAAc                                    >  1:930334/1‑140 (MQ=255)                                                                                                    gtgAAACTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACg                                   <  2:865615/140‑1 (MQ=255)                                                                                                       aaaCTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCt                                >  2:304057/1‑140 (MQ=255)                                                                                                         aCTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGa                              <  2:593402/140‑1 (MQ=255)                                                                                                         aCTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGa                              <  2:339973/140‑1 (MQ=255)                                                                                                          cTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGAt                             >  1:586097/1‑140 (MQ=255)                                                                                                          cTTAACCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGAt                             >  1:611258/1‑140 (MQ=255)                                                                                                             aaCCCGACCTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGc                          >  2:393943/1‑140 (MQ=255)                                                                                                                     cTTACTGGGGTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGTTGGCACTGGCaa                  >  1:336846/1‑140 (MQ=255)                                                                                                                              gTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAAt         >  1:500748/1‑140 (MQ=255)                                                                                                                              gTACGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAAt         >  1:963045/1‑140 (MQ=255)                                                                                                                                aCGCGCGTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCa       >  2:755422/1‑140 (MQ=255)                                                                                                                                   cgcgTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTc    >  1:521130/1‑140 (MQ=255)                                                                                                                                     cgTGCTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCATGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCgg  >  2:711557/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCGG  >  NC_000913/3016717‑3016988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑