Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,054,695	T→C	D49G (GAC→GGC)	pepP ←	proline aminopeptidase P II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,054,695	0	T	C	100.0%	187.4 / NA	52	D49G (GAC→GGC)	pepP	proline aminopeptidase P II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/26);  total (26/26)

AGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATAT  >  NC_000913/3054561‑3054831                                                                                                                                       |                                                                                                                                         aGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGCAGTACCAGAAGCCACTg                                                                                                                                     >  2:960393/1‑140 (MQ=255) aGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCa                                                                                                                                        >  1:296225/1‑137 (MQ=255) aGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTg                                                                                                                                     >  2:597106/1‑140 (MQ=255)      cGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTg                                                                                                                                >  2:843028/1‑140 (MQ=255)       ggCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGa                                                                                                                               <  1:838627/140‑1 (MQ=255)       ggCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGa                                                                                                                               <  1:597106/140‑1 (MQ=255)            aaCCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATa                                                                                                                          >  1:272293/1‑140 (MQ=255)            aaCCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATa                                                                                                                          <  2:397417/140‑1 (MQ=255)            aaCCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATa                                                                                                                          <  2:326876/140‑1 (MQ=255)                       cgccgTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGc                                                                                                               >  1:1034391/1‑140 (MQ=255)                         ccgTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTg                                                                                                             >  2:1067990/1‑140 (MQ=255)                           gTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTc                                                                                                           <  2:272293/140‑1 (MQ=255)                            tCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCg                                                                                                          >  2:352087/1‑140 (MQ=255)                             cAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCgg                                                                                                         <  1:1062193/140‑1 (MQ=255)                             cAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCgg                                                                                                         <  2:927122/140‑1 (MQ=255)                              aGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGgc                                                                                                        >  2:499663/1‑140 (MQ=255)                                 tCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGgcg                                                                                                       >  2:320181/1‑138 (MQ=255)                                 tCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGgcg                                                                                                       <  1:320181/138‑1 (MQ=255)                                 tCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCt                                                                                                     <  2:1040982/140‑1 (MQ=255)                                 tCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCt                                                                                                     <  2:135369/140‑1 (MQ=255)                                  cgcgAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTa                                                                                                    <  1:33601/140‑1 (MQ=255)                                  cgcgAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTa                                                                                                    <  2:465718/140‑1 (MQ=255)                                       aCGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACgtgt                                                                                               <  1:1067990/140‑1 (MQ=255)                                                     aaCGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTgcag                                                                                 >  1:357570/1‑140 (MQ=255)                                                       cGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTgcagca                                                                               >  2:458060/1‑140 (MQ=255)                                                                      tgtCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCgcggcg                                                                >  1:962800/1‑140 (MQ=255)                                                                      tgtCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCgcggcg                                                                >  1:467370/1‑140 (MQ=255)                                                                      ggtCATCGCCTTGAATCAGGACCACCACCGATAAGGGTTCGGTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCgcggcg                                                                >  2:784066/2‑140 (MQ=255)                                                                        tCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCt                                                              >  1:907861/1‑140 (MQ=255)                                                                        tCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCt                                                              >  2:291109/1‑140 (MQ=255)                                                                         cATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTg                                                             >  1:775192/1‑140 (MQ=255)                                                                         cATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTg                                                             >  2:244172/1‑140 (MQ=255)                                                                          ctCGCTTTTAATCAGCCCCAGCACCGGTTCCGGGTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGGCGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGc                                                            <  2:804147/139‑1 (MQ=255)                                                                             gCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCgg                                                         <  1:244172/140‑1 (MQ=255)                                                                               ttttAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGt                                                       <  2:357570/140‑1 (MQ=255)                                                                                tttAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGtt                                                      >  1:144217/1‑140 (MQ=255)                                                                                     tCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCAtt                                                 <  2:907861/140‑1 (MQ=255)                                                                                              gcaccGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTccacc                                        <  1:803440/140‑1 (MQ=255)                                                                                               caccGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTccacca                                       <  1:352087/140‑1 (MQ=255)                                                                                               caccGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTccacca                                       >  2:315453/1‑140 (MQ=255)                                                                                                   gCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGc                                   >  1:273207/1‑140 (MQ=255)                                                                                                   gCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGc                                   >  2:844714/1‑140 (MQ=255)                                                                                                    cTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCAtt                                                 <  2:72335/125‑1 (MQ=255)                                                                                                    cTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCAtt                                                 >  1:72335/1‑125 (MQ=255)                                                                                                        cGGTTCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGAc                              >  2:608165/1‑140 (MQ=255)                                                                                                            tCGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCg                          <  2:144217/140‑1 (MQ=255)                                                                                                             cGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGa                         <  2:467370/140‑1 (MQ=255)                                                                                                             cGTTAAAGCCGGTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGa                         <  2:25417/140‑1 (MQ=255)                                                                                                                        gTGAAGTACCAGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAActc              >  1:318850/1‑140 (MQ=255)                                                                                                                                  aGAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGat    <  2:962800/140‑1 (MQ=255)                                                                                                                                   gAAGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGata   >  1:573780/1‑140 (MQ=255)                                                                                                                                    aaGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGatat  <  1:280130/140‑1 (MQ=255)                                                                                                                                    aaGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGatat  <  1:451120/140‑1 (MQ=255)                                                                                                                                    aaGCCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGatat  <  1:458060/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         AGCGACGGCCAAACCAGATCTCCGCCGTCAGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATAT  >  NC_000913/3054561‑3054831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑