Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,089,687	A→G	intergenic (+9/‑68)	galP → / → yggI	galactose:H(+) symporter/conserved protein YggI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,089,687	0	A	G	100.0%	176.9 / NA	51	intergenic (+9/‑68)	galP/yggI	galactose:H(+) symporter/conserved protein YggI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/23);  total (28/23)

GCTCTGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGC  >  NC_000913/3089550‑3089822                                                                                                                                          |                                                                                                                                        gCTCTGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGc                                                                                                                                       >  1:652345/1‑140 (MQ=255)  ctctGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCt                                                                                                                                      >  1:533989/1‑140 (MQ=255)   tctGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCtt                                                                                                                                     >  1:48704/1‑140 (MQ=255)     tGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCTCTCACGATTAATCTCCCCAGGCTtcc                                                                                                                                   >  1:755563/1‑140 (MQ=255)     tGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTtcc                                                                                                                                   >  1:692669/1‑140 (MQ=255)     tGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTtcc                                                                                                                                   >  1:685213/1‑140 (MQ=255)            aCTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACATAATCTGATGAAAGGTCGTAAACCGCGCGAAAAAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATc                                                                                                                            >  2:249934/1‑140 (MQ=255)               gTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATcgcg                                                                                                                         >  1:1072658/1‑140 (MQ=255)                  tATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCggag                                                                                                                      <  2:692669/140‑1 (MQ=255)                      ctgctgACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAg                                                                                                                  <  2:533989/140‑1 (MQ=255)                        gctgACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGcc                                                                                                                >  2:458479/1‑140 (MQ=255)                        gctgACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGcc                                                                                                                >  2:113193/1‑140 (MQ=255)                        gctgACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGcc                                                                                                                >  2:31730/1‑140 (MQ=255)                        gctgACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGcc                                                                                                                >  2:204556/1‑140 (MQ=255)                                tgaggCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTg                                                                                                        <  1:922760/137‑1 (MQ=255)                                   ggCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAg                                                                                                     >  2:969844/1‑140 (MQ=255)                                              gAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTttct                                                                                          >  1:55842/1‑140 (MQ=255)                                              gAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTttct                                                                                          <  2:242740/140‑1 (MQ=255)                                              gAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTttct                                                                                          <  1:113193/140‑1 (MQ=255)                                              gAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTttct                                                                                          >  1:927502/1‑140 (MQ=255)                                                aaCCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTttcttc                                                                                        <  1:671226/140‑1 (MQ=255)                                                 aCCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCg                                                                                       <  1:163096/140‑1 (MQ=255)                                                   cAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGct                                                                                     >  1:1020019/1‑140 (MQ=255)                                                             tCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTg                                                                           <  1:204556/140‑1 (MQ=255)                                                                  ggAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCt                                                                      <  2:1072658/140‑1 (MQ=255)                                                                         atTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGaaaa                                                               >  2:386839/1‑140 (MQ=255)                                                                               cGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATccc                                                         >  2:988639/1‑140 (MQ=255)                                                                                gTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCg                                                        >  1:227603/1‑140 (MQ=255)                                                                                 tAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGt                                                       <  1:386839/140‑1 (MQ=255)                                                                                 tAATCTGATGAAAGGTCGTAAACCGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGt                                                       <  2:106096/140‑1 (MQ=255)                                                                                  aaTCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGtc                                                      <  2:767982/140‑1 (MQ=255)                                                                                  aaTCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGtc                                                      >  2:132452/1‑140 (MQ=255)                                                                                         tgaAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTAt                                               >  1:982373/1‑140 (MQ=255)                                                                                         tgaAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTAt                                               <  2:55842/140‑1 (MQ=255)                                                                                              ggTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCa                                          <  1:988639/140‑1 (MQ=255)                                                                                               gTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCAt                                         >  1:588325/1‑140 (MQ=255)                                                                                                   tAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCaa                                     >  2:395731/1‑140 (MQ=255)                                                                                                   tAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCaa                                     <  1:31730/140‑1 (MQ=255)                                                                                                    aaaCTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAAc                                    <  1:373782/140‑1 (MQ=255)                                                                                                    aaaCTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAAc                                    <  2:927502/140‑1 (MQ=255)                                                                                                     aaCTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACa                                   <  2:48704/140‑1 (MQ=255)                                                                                                       cTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAgg                                 <  1:596133/140‑1 (MQ=255)                                                                                                       cTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAgg                                 <  1:458479/140‑1 (MQ=255)                                                                                                       cTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAgg                                 <  2:685213/140‑1 (MQ=255)                                                                                                          cgcgAAATAGGCGCTCACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCg                              >  2:336662/1‑140 (MQ=255)                                                                                                                         cACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGc               >  1:172293/1‑140 (MQ=255)                                                                                                                         cACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGc               >  1:233153/1‑140 (MQ=255)                                                                                                                         cACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGc               >  2:483573/1‑140 (MQ=255)                                                                                                                         cACGATTAATCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGc               >  2:47283/1‑140 (MQ=255)                                                                                                                                 aTCTCCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGaaaaa       <  1:969844/140‑1 (MQ=255)                                                                                                                                   ctcCCCAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACt     <  2:869011/140‑1 (MQ=255)                                                                                                                                      cccAGGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGTCTGCGGGAAAAACTCGc  >  2:959917/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GCTCTGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATCGCGGAGGAAGCCACCTCTTGCAGTCATCTTTTCTTCGCTCTATCCTCTGCCGCTATGAAAACATCCCGTCTCCCTATCGCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGC  >  NC_000913/3089550‑3089822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑