Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,222,943	T→C	V104A (GTC→GCC)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,222,943	0	T	C	100.0%	255.9 / NA	69	V104A (GTC→GCC)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/31);  total (38/31)

TACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGT  >  NC_000913/3222808‑3223075                                                                                                                                        |                                                                                                                                     tACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCcccc                                                                                                                                  <  1:417450/140‑1 (MQ=255) tACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCcccc                                                                                                                                  >  1:927620/1‑140 (MQ=255)  aCCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCa                                                                                                                                 >  2:387643/1‑140 (MQ=255)  aCCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGAGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTATCCGTTCCCCATCGATGTGCCGTTTGCCCCCa                                                                                                                                 >  2:953289/1‑140 (MQ=255)    cAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGc                                                                                                                               <  1:953289/140‑1 (MQ=255)          ccTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAAc                                                                                                                         >  2:251177/1‑140 (MQ=255)            ttCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAAccc                                                                                                                       >  1:328411/1‑140 (MQ=255)            ttCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAAccc                                                                                                                       >  2:1002321/1‑140 (MQ=255)            ttCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAAccc                                                                                                                       >  1:977294/1‑140 (MQ=255)            ttCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAAccc                                                                                                                       >  1:714703/1‑140 (MQ=255)               aCCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAAc                                                                                                                    <  1:135857/140‑1 (MQ=255)               aCCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAAc                                                                                                                    >  1:272672/1‑140 (MQ=255)                 ctctGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCg                                                                                                                  <  2:341017/140‑1 (MQ=255)                 ctctGAGTTAATGGCTGACGGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCg                                                                                                                  <  2:308178/140‑1 (MQ=255)                   ctGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCg                                                                                                                  >  1:2046/1‑138 (MQ=255)                   ctGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCg                                                                                                                  <  2:2046/138‑1 (MQ=255)                   ctGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGt                                                                                                                <  2:506647/140‑1 (MQ=255)                    tGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTg                                                                                                               >  2:575499/1‑140 (MQ=255)                      aGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGAt                                                                                                                            <  2:1009565/125‑1 (MQ=255)                      aGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGAt                                                                                                                            >  1:1009565/1‑125 (MQ=255)                          aaTGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATc                                                                                                         <  2:927620/140‑1 (MQ=255)                           aTGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAAc                                                                                                                    <  1:269054/128‑1 (MQ=255)                           aTGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAAc                                                                                                                    >  2:269054/1‑128 (MQ=255)                              gcgggcggggggCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACg                                                                                                     <  2:714703/133‑1 (MQ=255)                              gCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACg                                                                                                     >  2:22600/1‑140 (MQ=255)                              gCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACg                                                                                                     <  1:387643/140‑1 (MQ=255)                                tGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAAc                                                                                                                    <  1:285341/123‑1 (MQ=255)                                tGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAAc                                                                                                                    >  2:285341/1‑123 (MQ=255)                                tGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTa                                                                                                   >  1:26766/1‑140 (MQ=255)                                        ggCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCG‑TTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACg                                                                                                     <  2:193950/129‑1 (MQ=255)                                        ggCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCG‑TTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACg                                                                                                     >  1:193950/1‑129 (MQ=255)                                              ttACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGc                                                                                     >  2:95437/1‑140 (MQ=255)                                                aCCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGa                                                                                   >  1:703418/1‑140 (MQ=255)                                                    tCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGc                                                                               >  1:362373/1‑140 (MQ=255)                                                     ccccGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCAcc                                                                                           >  2:653604/1‑127 (MQ=255)                                                     ccccGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCAcc                                                                                           <  1:653604/127‑1 (MQ=255)                                                      cccGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTg                                                                             <  2:362373/140‑1 (MQ=255)                                                      cccGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTg                                                                             <  1:809975/140‑1 (MQ=255)                                                       ccGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTgg                                                                            >  1:1073521/1‑140 (MQ=255)                                                       ccGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTgg                                                                            <  2:915933/140‑1 (MQ=255)                                                       ccGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTgg                                                                            >  1:368418/1‑140 (MQ=255)                                                        cGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGc                                                                           <  1:768039/140‑1 (MQ=255)                                                        cGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGc                                                                           >  1:372208/1‑140 (MQ=255)                                                        cGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCGCAGGGACGGCTGGc                                                                           >  2:934421/1‑140 (MQ=255)                                                         gCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCa                                                                          >  1:227339/1‑140 (MQ=255)                                                         gCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCa                                                                          <  2:372208/140‑1 (MQ=255)                                                           cATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAg                                                                                      >  1:735374/1‑126 (MQ=255)                                                           cATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAg                                                                                      <  2:735374/126‑1 (MQ=255)                                                           cATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAgg                                                                        <  2:118626/140‑1 (MQ=255)                                                            aTGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAggg                                                                       <  2:272672/140‑1 (MQ=255)                                                              gtgGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTa                                                                     >  2:410010/1‑140 (MQ=255)                                                                    aaTGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGa                                                               <  2:26766/140‑1 (MQ=255)                                                                          aGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGa                                                         <  1:22600/140‑1 (MQ=255)                                                                                 ggCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAAtt                                                  >  2:125178/1‑140 (MQ=255)                                                                                      aCTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACg                                             >  1:426582/1‑140 (MQ=255)                                                                                                 ccGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAAcc                                  >  1:999540/1‑140 (MQ=255)                                                                                                  cgacgaAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCt                                 <  2:426582/140‑1 (MQ=255)                                                                                                  cgacgaAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCt                                 <  1:688308/140‑1 (MQ=255)                                                                                                  cgacgaAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCt                                 >  2:688308/1‑140 (MQ=255)                                                                                                         ggTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGa                          >  2:83238/1‑140 (MQ=255)                                                                                                          gTTTTCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGaa                         <  2:999540/140‑1 (MQ=255)                                                                                                             ttCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTc                      >  2:298278/1‑140 (MQ=255)                                                                                                              tCCGTTCCCCATCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCt                     <  1:575499/140‑1 (MQ=255)                                                                                                                         tCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGt          <  1:1002321/140‑1 (MQ=255)                                                                                                                         tCGATGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGt          >  1:733647/1‑140 (MQ=255)                                                                                                                            aTGTGCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAg       >  1:847479/1‑140 (MQ=255)                                                                                                                             tgtgCCGTTTGCCCCCAGCGATAATCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGt      >  1:378606/1‑140 (MQ=255)                                                                                                                             tgtgCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTa               >  1:19864/1‑131 (MQ=255)                                                                                                                             tgtgCCGTTTGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTa               <  2:19864/131‑1 (MQ=255)                                                                                                                                     ttGCCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTAtgt  <  2:7673/136‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     TACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGT  >  NC_000913/3222808‑3223075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑